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Article: Characterization of the receptors for chicken GHRH and GHRH-related peptides: Identification of a novel receptor for GHRH and the receptor for GHRH-LP (PRP)

TitleCharacterization of the receptors for chicken GHRH and GHRH-related peptides: Identification of a novel receptor for GHRH and the receptor for GHRH-LP (PRP)
Authors
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/domaniend
Citation
Domestic Animal Endocrinology, 2010, v. 38 n. 1, p. 13-31 How to Cite?
AbstractGrowth hormone-releasing hormone and its structurally related peptides, GHRH-like peptide (GHRH-LP) (also called PRP), peptide histidine-isoleucine (PHI), vasoactive intestinal polypeptide (VIP), and pituitary adenylate cyclase-activating polypeptide (PACAP), have been reported to play important physiological roles in pituitary and extrapituitary tissues of vertebrates; however, little is known about the identity of these GHRH-related peptide receptors in birds. In this study, 6 receptors for GHRH and GHRH-related peptides (cGHRHR1, cGHRHR2, cGHRH-LPR, cPAC1, cVPAC1, and cVPAC2) were cloned from chicken brain or pituitary, and their functionalities were examined in Chinese hamster ovary (CHO) cells using a pGL3-CRE-luciferase reporter system. Results showed that: (1) all receptors are G protein-coupled receptors functionally coupled to the intracellular PKA signaling pathway; (2) 2 GHRH receptors (cGHRHR1 and cGHRHR2) were identified, and both receptors could be potently activated by cGHRH; (3) cGHRH-LP could activate its specific receptor cGHRH-LPR (cPRP-R), and it also activated cGHRHR1 and cGHRHR2; and (4) PACAP could potently activate its receptors cPAC1, cVPAC1 and cVPAC2; however, cVPAC1 and cVPAC2 could also be effectively activated by cVIP and tPHI, indicating that they can serve as VIP receptors and potential PHI receptors. Using a reverse transcription polymerase chain reaction assay, we further examined the mRNA expression of these receptors in adult chicken tissues. The expressions of cGHRHR1, cGHRHR2, and cGHRH-LPR are restricted mainly to the pituitary and/or brain, whereas cPAC1, cVPAC1, and cVPAC2 are expressed in most of the tissues examined. Collectively, our study identified the receptors for chicken GHRH and GHRH-related peptides, including a novel GHRH receptor (cGHRHR2), and established a basis to elucidate the roles of these peptides in target tissues. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179165
ISSN
2015 Impact Factor: 1.613
2015 SCImago Journal Rankings: 0.751
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_US
dc.contributor.authorLi, Jen_US
dc.contributor.authorWang, CYen_US
dc.contributor.authorKwok, AYen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:52:29Z-
dc.date.available2012-12-19T09:52:29Z-
dc.date.issued2010en_US
dc.identifier.citationDomestic Animal Endocrinology, 2010, v. 38 n. 1, p. 13-31en_US
dc.identifier.issn0739-7240en_US
dc.identifier.urihttp://hdl.handle.net/10722/179165-
dc.description.abstractGrowth hormone-releasing hormone and its structurally related peptides, GHRH-like peptide (GHRH-LP) (also called PRP), peptide histidine-isoleucine (PHI), vasoactive intestinal polypeptide (VIP), and pituitary adenylate cyclase-activating polypeptide (PACAP), have been reported to play important physiological roles in pituitary and extrapituitary tissues of vertebrates; however, little is known about the identity of these GHRH-related peptide receptors in birds. In this study, 6 receptors for GHRH and GHRH-related peptides (cGHRHR1, cGHRHR2, cGHRH-LPR, cPAC1, cVPAC1, and cVPAC2) were cloned from chicken brain or pituitary, and their functionalities were examined in Chinese hamster ovary (CHO) cells using a pGL3-CRE-luciferase reporter system. Results showed that: (1) all receptors are G protein-coupled receptors functionally coupled to the intracellular PKA signaling pathway; (2) 2 GHRH receptors (cGHRHR1 and cGHRHR2) were identified, and both receptors could be potently activated by cGHRH; (3) cGHRH-LP could activate its specific receptor cGHRH-LPR (cPRP-R), and it also activated cGHRHR1 and cGHRHR2; and (4) PACAP could potently activate its receptors cPAC1, cVPAC1 and cVPAC2; however, cVPAC1 and cVPAC2 could also be effectively activated by cVIP and tPHI, indicating that they can serve as VIP receptors and potential PHI receptors. Using a reverse transcription polymerase chain reaction assay, we further examined the mRNA expression of these receptors in adult chicken tissues. The expressions of cGHRHR1, cGHRHR2, and cGHRH-LPR are restricted mainly to the pituitary and/or brain, whereas cPAC1, cVPAC1, and cVPAC2 are expressed in most of the tissues examined. Collectively, our study identified the receptors for chicken GHRH and GHRH-related peptides, including a novel GHRH receptor (cGHRHR2), and established a basis to elucidate the roles of these peptides in target tissues. © 2009 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/domanienden_US
dc.relation.ispartofDomestic Animal Endocrinologyen_US
dc.rightsDomestic Animal Endocrinology. Copyright © Elsevier Inc.-
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCho Cellsen_US
dc.subject.meshChickens - Genetics - Metabolismen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshCricetulusen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptide - Genetics - Metabolismen_US
dc.subject.meshRna - Chemistry - Geneticsen_US
dc.subject.meshReceptors, Neuropeptide - Genetics - Metabolismen_US
dc.subject.meshReceptors, Pituitary Hormone-Regulating Hormone - Genetics - Metabolismen_US
dc.subject.meshReceptors, Vasoactive Intestinal Peptide, Type Ii - Genetics - Metabolismen_US
dc.subject.meshReceptors, Vasoactive Intestinal Polypeptide, Type I - Genetics - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Veterinaryen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Analysis, Dnaen_US
dc.subject.meshTransfection - Veterinaryen_US
dc.titleCharacterization of the receptors for chicken GHRH and GHRH-related peptides: Identification of a novel receptor for GHRH and the receptor for GHRH-LP (PRP)en_US
dc.typeArticleen_US
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityWang, Y=rp00801en_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.domaniend.2009.07.008en_US
dc.identifier.pmid19748756en_US
dc.identifier.scopuseid_2-s2.0-70449672388en_US
dc.identifier.hkuros167649-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70449672388&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume38en_US
dc.identifier.issue1en_US
dc.identifier.spage13en_US
dc.identifier.epage31en_US
dc.identifier.isiWOS:000277928300002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWang, Y=36062525200en_US
dc.identifier.scopusauthoridLi, J=27169727600en_US
dc.identifier.scopusauthoridWang, CY=16065173000en_US
dc.identifier.scopusauthoridKwok, AY=35484587700en_US
dc.identifier.scopusauthoridZhang, X=7410277503en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US
dc.identifier.citeulike5707015-

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