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Article: Overexpressing AtPAP15 enhances phosphorus efficiency in soybean

TitleOverexpressing AtPAP15 enhances phosphorus efficiency in soybean
Authors
Issue Date2009
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 2009, v. 151 n. 1, p. 233-240 How to Cite?
AbstractLow phosphorus (P) availability is a major constraint to crop growth and production, including soybean (Glycine max), on a global scale. However, 50% to 80% of the total P in agricultural soils exists as organic phosphate, which is unavailable to plants unless hydrolyzed to release inorganic phosphate. One strategy for improving crop P nutrition is the enhanced activity of acid phosphatases (APases) to obtain or remobilize inorganic phosphate from organic P sources. In this study, we overexpressed an Arabidopsis (Arabidopsis thaliana) purple APase gene (AtPAP15) containing a carrot (Daucus carota) extracellular targeting peptide in soybean hairy roots and found that the APase activity was increased by 1.5-fold in transgenic hairy roots. We subsequently transformed soybean plants with AtPAP15 and studied three homozygous overexpression lines of AtPAP15. The three transgenic lines exhibited significantly improved P efficiency with 117.8%, 56.5%, and 57.8% increases in plant dry weight, and 90.1%, 18.2%, and 62.6% increases in plant P content, respectively, as compared with wild-type plants grown on sand culture containing phytate as the sole P source. The transgenic soybean lines also exhibited a significant level of APase and phytase activity in leaves and root exudates, respectively. Furthermore, the transgenic lines exhibited improved yields when grown on acid soils, with 35.9%, 41.0%, and 59.0% increases in pod number per plant, and 46.0%, 48.3%, and 66.7% increases in seed number per plant. Taken together, to our knowledge, our study is the first report on the improvement of P efficiency in soybean through constitutive expression of a plant APase gene. These findings could have significant implications for improving crop yield on soils low in available P, which is a serious agricultural limitation worldwide. © 2009 American Society of Plant Biologists.
Persistent Identifierhttp://hdl.handle.net/10722/179158
ISSN
2015 Impact Factor: 6.28
2015 SCImago Journal Rankings: 3.642
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorTian, Jen_US
dc.contributor.authorLim, BLen_US
dc.contributor.authorYan, Xen_US
dc.contributor.authorLiao, Hen_US
dc.date.accessioned2012-12-19T09:52:26Z-
dc.date.available2012-12-19T09:52:26Z-
dc.date.issued2009en_US
dc.identifier.citationPlant Physiology, 2009, v. 151 n. 1, p. 233-240en_US
dc.identifier.issn0032-0889en_US
dc.identifier.urihttp://hdl.handle.net/10722/179158-
dc.description.abstractLow phosphorus (P) availability is a major constraint to crop growth and production, including soybean (Glycine max), on a global scale. However, 50% to 80% of the total P in agricultural soils exists as organic phosphate, which is unavailable to plants unless hydrolyzed to release inorganic phosphate. One strategy for improving crop P nutrition is the enhanced activity of acid phosphatases (APases) to obtain or remobilize inorganic phosphate from organic P sources. In this study, we overexpressed an Arabidopsis (Arabidopsis thaliana) purple APase gene (AtPAP15) containing a carrot (Daucus carota) extracellular targeting peptide in soybean hairy roots and found that the APase activity was increased by 1.5-fold in transgenic hairy roots. We subsequently transformed soybean plants with AtPAP15 and studied three homozygous overexpression lines of AtPAP15. The three transgenic lines exhibited significantly improved P efficiency with 117.8%, 56.5%, and 57.8% increases in plant dry weight, and 90.1%, 18.2%, and 62.6% increases in plant P content, respectively, as compared with wild-type plants grown on sand culture containing phytate as the sole P source. The transgenic soybean lines also exhibited a significant level of APase and phytase activity in leaves and root exudates, respectively. Furthermore, the transgenic lines exhibited improved yields when grown on acid soils, with 35.9%, 41.0%, and 59.0% increases in pod number per plant, and 46.0%, 48.3%, and 66.7% increases in seed number per plant. Taken together, to our knowledge, our study is the first report on the improvement of P efficiency in soybean through constitutive expression of a plant APase gene. These findings could have significant implications for improving crop yield on soils low in available P, which is a serious agricultural limitation worldwide. © 2009 American Society of Plant Biologists.en_US
dc.languageengen_US
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.orgen_US
dc.relation.ispartofPlant Physiologyen_US
dc.subject.mesh6-Phytase - Metabolismen_US
dc.subject.meshAcid Phosphatase - Genetics - Metabolismen_US
dc.subject.meshArabidopsis Proteins - Genetics - Metabolismen_US
dc.subject.meshMultienzyme Complexes - Genetics - Metabolismen_US
dc.subject.meshPhosphorus - Metabolismen_US
dc.subject.meshPlant Roots - Genetics - Metabolismen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshSoybeans - Enzymology - Genetics - Growth & Development - Metabolismen_US
dc.titleOverexpressing AtPAP15 enhances phosphorus efficiency in soybeanen_US
dc.typeArticleen_US
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_US
dc.identifier.authorityLim, BL=rp00744en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1104/pp.109.138891en_US
dc.identifier.pmid19587103-
dc.identifier.pmcidPMC2736008-
dc.identifier.scopuseid_2-s2.0-70349213934en_US
dc.identifier.hkuros164344-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349213934&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume151en_US
dc.identifier.issue1en_US
dc.identifier.spage233en_US
dc.identifier.epage240en_US
dc.identifier.isiWOS:000269522200019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWang, X=8695873600en_US
dc.identifier.scopusauthoridWang, Y=7601494131en_US
dc.identifier.scopusauthoridTian, J=55111185900en_US
dc.identifier.scopusauthoridLim, BL=7201983917en_US
dc.identifier.scopusauthoridYan, X=7403596595en_US
dc.identifier.scopusauthoridLiao, H=55121516700en_US

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