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Article: Protein profiles in zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonate

TitleProtein profiles in zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonate
Authors
Issue Date2009
PublisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/
Citation
Toxicological Sciences, 2009, v. 110 n. 2, p. 334-340 How to Cite?
AbstractPerfluorooctane sulfonate (PFOS) is widely distributed and persistent in the environment and in wildlife, and it has the potential for developmental toxicity. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the proteins that are differentially expressed in zebrafish embryos exposed to 0.5 mg/l PFOS until 192 h postfertilization. Two-dimensional electrophoresis coupled with mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that 69 proteins showed altered expression in the treatment group compared to the control group with either increase or decrease in expression levels (more than twofold difference). Of the 69 spots corresponding to the proteins with altered expression, 38 were selected and subjected to matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (TOF/TOF) analysis; 18 proteins were identified in this analysis. These proteins can be categorized into diverse functional classes such as detoxification, energy metabolism, lipid transport/steroid metabolic process, cell structure, signal transduction, and apoptosis. Overall, proteomic analysis using zebrafish embryos serves as an in vivo model in environmental risk assessment and provides insight into the molecular events in PFOS-induced developmental toxicity. © The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179144
ISSN
2015 Impact Factor: 3.88
2015 SCImago Journal Rankings: 1.686
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShi, Xen_US
dc.contributor.authorYeung, LWYen_US
dc.contributor.authorLam, PKSen_US
dc.contributor.authorWu, RSSen_US
dc.contributor.authorZhou, Ben_US
dc.date.accessioned2012-12-19T09:52:20Z-
dc.date.available2012-12-19T09:52:20Z-
dc.date.issued2009en_US
dc.identifier.citationToxicological Sciences, 2009, v. 110 n. 2, p. 334-340en_US
dc.identifier.issn1096-6080en_US
dc.identifier.urihttp://hdl.handle.net/10722/179144-
dc.description.abstractPerfluorooctane sulfonate (PFOS) is widely distributed and persistent in the environment and in wildlife, and it has the potential for developmental toxicity. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the proteins that are differentially expressed in zebrafish embryos exposed to 0.5 mg/l PFOS until 192 h postfertilization. Two-dimensional electrophoresis coupled with mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that 69 proteins showed altered expression in the treatment group compared to the control group with either increase or decrease in expression levels (more than twofold difference). Of the 69 spots corresponding to the proteins with altered expression, 38 were selected and subjected to matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (TOF/TOF) analysis; 18 proteins were identified in this analysis. These proteins can be categorized into diverse functional classes such as detoxification, energy metabolism, lipid transport/steroid metabolic process, cell structure, signal transduction, and apoptosis. Overall, proteomic analysis using zebrafish embryos serves as an in vivo model in environmental risk assessment and provides insight into the molecular events in PFOS-induced developmental toxicity. © The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/en_US
dc.relation.ispartofToxicological Sciencesen_US
dc.subject.meshAlkanesulfonic Acids - Toxicityen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Markers - Metabolismen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshEmbryo, Nonmammalian - Drug Effects - Metabolismen_US
dc.subject.meshEnvironmental Monitoring - Methodsen_US
dc.subject.meshFluorocarbons - Toxicityen_US
dc.subject.meshPeptide Mappingen_US
dc.subject.meshProteomics - Methodsen_US
dc.subject.meshRisk Assessmenten_US
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshWater Pollutants, Chemical - Toxicityen_US
dc.subject.meshZebrafish - Embryology - Metabolismen_US
dc.subject.meshZebrafish Proteins - Metabolismen_US
dc.titleProtein profiles in zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonateen_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/toxsci/kfp111en_US
dc.identifier.pmid19474218en_US
dc.identifier.scopuseid_2-s2.0-67650770098en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67650770098&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume110en_US
dc.identifier.issue2en_US
dc.identifier.spage334en_US
dc.identifier.epage340en_US
dc.identifier.isiWOS:000267889700008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridShi, X=16067334700en_US
dc.identifier.scopusauthoridYeung, LWY=9735175200en_US
dc.identifier.scopusauthoridLam, PKS=7202365776en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US
dc.identifier.scopusauthoridZhou, B=7401906781en_US

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