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Article: UDP-glucose pyrophosphorylase2 (OsUgp2), a pollen-preferential gene in rice, plays a critical role in starch accumulation during pollen maturation

TitleUDP-glucose pyrophosphorylase2 (OsUgp2), a pollen-preferential gene in rice, plays a critical role in starch accumulation during pollen maturation
Authors
KeywordsPollen
Rice
Udp-Glucose Pyrophosphorylase
Issue Date2009
PublisherScience China Press. The Journal's web site is located at http://www.springerlink.com/content/1001-6538/
Citation
Chinese Science Bulletin, 2009, v. 54 n. 2, p. 234-243 How to Cite?
AbstractUDP-glucose pyrophosphorylase (UGPase) is predominantly present and plays significant role in carbohydrate metabolism in plants. Two homologous UGPase genes, OsUgp1 and OsUgp2, exist in rice genome. OsUgp1 has recently been reported to be essential for callose deposition during pollen mother cell and meiosis stages as well as for seed carbohydrate metabolism. In this study, a full-length cDNA of OsUgp2 was isolated from rice anther. Northern blot and RNA in situ hybridization indicated that the expression of OsUgp2 was preferentially in pollen and developmentally regulated. No transcripts were found in leaf, stem, lemma/palea, ripening grain and florets before the uninucleate microspore developmental stage, but a large quantity of OsUgp2 mRNA was found in pollen at the binucleate and mature stages. The immunolocalization of OsUgp2 showed a similar expression pattern to that by RNA in situ hybridization. The function of OsUgp2 was investigated by dsRNA-mediated transcriptional gene silencing. The pollen fertility of 16 independent transgenic rice plants was found between 25% and 90%, which was correlated with the amount of OsUgp2 mRNA. The results of morphological changes and starch variation during pollen development in transgenic rice showed that the abnormal feature of pollen development appeared after the uninucleate microspore stage. Starch failed to accumulate in pollen and thus led to sterile pollens. These results demonstrated that OsUgp2 is a pollen-preferential "late gene" and plays a key role during pollen maturation, especially for starch accumulation. OsUgp2 complements OsUgp1 to fulfill the UGPase's functions necessary for the full process of pollen development. © 2009 Science in China Press and Springer-Verlag GmbH.
Persistent Identifierhttp://hdl.handle.net/10722/179116
ISSN
2015 Impact Factor: 1.789
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMu, Hen_US
dc.contributor.authorKe, JHen_US
dc.contributor.authorLiu, Wen_US
dc.contributor.authorZhuang, CXen_US
dc.contributor.authorYip, WKen_US
dc.date.accessioned2012-12-19T09:52:05Z-
dc.date.available2012-12-19T09:52:05Z-
dc.date.issued2009en_US
dc.identifier.citationChinese Science Bulletin, 2009, v. 54 n. 2, p. 234-243en_US
dc.identifier.issn1001-6538en_US
dc.identifier.urihttp://hdl.handle.net/10722/179116-
dc.description.abstractUDP-glucose pyrophosphorylase (UGPase) is predominantly present and plays significant role in carbohydrate metabolism in plants. Two homologous UGPase genes, OsUgp1 and OsUgp2, exist in rice genome. OsUgp1 has recently been reported to be essential for callose deposition during pollen mother cell and meiosis stages as well as for seed carbohydrate metabolism. In this study, a full-length cDNA of OsUgp2 was isolated from rice anther. Northern blot and RNA in situ hybridization indicated that the expression of OsUgp2 was preferentially in pollen and developmentally regulated. No transcripts were found in leaf, stem, lemma/palea, ripening grain and florets before the uninucleate microspore developmental stage, but a large quantity of OsUgp2 mRNA was found in pollen at the binucleate and mature stages. The immunolocalization of OsUgp2 showed a similar expression pattern to that by RNA in situ hybridization. The function of OsUgp2 was investigated by dsRNA-mediated transcriptional gene silencing. The pollen fertility of 16 independent transgenic rice plants was found between 25% and 90%, which was correlated with the amount of OsUgp2 mRNA. The results of morphological changes and starch variation during pollen development in transgenic rice showed that the abnormal feature of pollen development appeared after the uninucleate microspore stage. Starch failed to accumulate in pollen and thus led to sterile pollens. These results demonstrated that OsUgp2 is a pollen-preferential "late gene" and plays a key role during pollen maturation, especially for starch accumulation. OsUgp2 complements OsUgp1 to fulfill the UGPase's functions necessary for the full process of pollen development. © 2009 Science in China Press and Springer-Verlag GmbH.en_US
dc.languageengen_US
dc.publisherScience China Press. The Journal's web site is located at http://www.springerlink.com/content/1001-6538/en_US
dc.relation.ispartofChinese Science Bulletinen_US
dc.subjectPollenen_US
dc.subjectRiceen_US
dc.subjectUdp-Glucose Pyrophosphorylaseen_US
dc.titleUDP-glucose pyrophosphorylase2 (OsUgp2), a pollen-preferential gene in rice, plays a critical role in starch accumulation during pollen maturationen_US
dc.typeArticleen_US
dc.identifier.emailYip, WK: wkyip@hkucc.hku.hken_US
dc.identifier.authorityYip, WK=rp00833en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11434-008-0568-yen_US
dc.identifier.scopuseid_2-s2.0-59849126832en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-59849126832&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume54en_US
dc.identifier.issue2en_US
dc.identifier.spage234en_US
dc.identifier.epage243en_US
dc.identifier.isiWOS:000263128500009-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridMu, H=7005762933en_US
dc.identifier.scopusauthoridKe, JH=26027917200en_US
dc.identifier.scopusauthoridLiu, W=36078712200en_US
dc.identifier.scopusauthoridZhuang, CX=7003322231en_US
dc.identifier.scopusauthoridYip, WK=7102784428en_US
dc.identifier.citeulike4030419-

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