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Article: An anther-specific dihydroflavonol 4-reductase-like gene (DRL1) is essential for male fertility in Arabidopsis

TitleAn anther-specific dihydroflavonol 4-reductase-like gene (DRL1) is essential for male fertility in Arabidopsis
Authors
Issue Date2009
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPH
Citation
New Phytologist, 2009, v. 181 n. 3, p. 576-587 How to Cite?
Abstract• Arabidopsis contains only one functional dihydroflavonol 4-reductase (DFR) gene, but several DFR-like genes encoding proteins with the conserved NAD(P)H binding domain. At4g35420, named DRL1 (Dihydroflavonol 4-reductase-like1), is a closely related homolog of the rice anther-specific gene OsDFR2 reported previously. • Two T-DNA mutants (drl1-1 and drl1-2) were found to have impaired pollen formation and seed production. Histological analysis revealed defective microspore development after tetrad release in both mutants. Microspore walls were found to rupture, releasing the protoplasts which eventually degenerated. The DRL1 promoter is anther-specific in closed flower buds. Promoter-GUS analysis in transgenic Arabidopsis revealed expression in tapetum, tetrads, and developing microspores, but not in mature anthers. Enhanced yellow fluorescent protein (EYFP)-localization analysis demonstrated that DRL1 is a soluble cytosolic protein that may also be localized in the nucleus. • Restoration of male fertility and seed formation was only achieved by a native promoter-DRL1 construct, but not by a 35S-DRL1 construct, demonstrating the importance of spatial and temporal specificities of DRL1 expression. • DRL1 may be involved in a novel metabolic pathway essential for pollen wall development. DRL1 homologs were identified as anther- and floral-specific expressed sequence tags from different species, suggesting that DRL1 may have a conserved functional role in male fertility in flowering plants. © The Authors (2008).
Persistent Identifierhttp://hdl.handle.net/10722/179112
ISSN
2015 Impact Factor: 7.21
2015 SCImago Journal Rankings: 3.603
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, LKen_US
dc.contributor.authorChu, Hen_US
dc.contributor.authorYip, WKen_US
dc.contributor.authorYeung, ECen_US
dc.contributor.authorLo, Cen_US
dc.date.accessioned2012-12-19T09:52:04Z-
dc.date.available2012-12-19T09:52:04Z-
dc.date.issued2009en_US
dc.identifier.citationNew Phytologist, 2009, v. 181 n. 3, p. 576-587en_US
dc.identifier.issn0028-646Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179112-
dc.description.abstract• Arabidopsis contains only one functional dihydroflavonol 4-reductase (DFR) gene, but several DFR-like genes encoding proteins with the conserved NAD(P)H binding domain. At4g35420, named DRL1 (Dihydroflavonol 4-reductase-like1), is a closely related homolog of the rice anther-specific gene OsDFR2 reported previously. • Two T-DNA mutants (drl1-1 and drl1-2) were found to have impaired pollen formation and seed production. Histological analysis revealed defective microspore development after tetrad release in both mutants. Microspore walls were found to rupture, releasing the protoplasts which eventually degenerated. The DRL1 promoter is anther-specific in closed flower buds. Promoter-GUS analysis in transgenic Arabidopsis revealed expression in tapetum, tetrads, and developing microspores, but not in mature anthers. Enhanced yellow fluorescent protein (EYFP)-localization analysis demonstrated that DRL1 is a soluble cytosolic protein that may also be localized in the nucleus. • Restoration of male fertility and seed formation was only achieved by a native promoter-DRL1 construct, but not by a 35S-DRL1 construct, demonstrating the importance of spatial and temporal specificities of DRL1 expression. • DRL1 may be involved in a novel metabolic pathway essential for pollen wall development. DRL1 homologs were identified as anther- and floral-specific expressed sequence tags from different species, suggesting that DRL1 may have a conserved functional role in male fertility in flowering plants. © The Authors (2008).en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/NPHen_US
dc.relation.ispartofNew Phytologisten_US
dc.rightsNew Phytologist. Copyright © Blackwell Publishing Ltd.-
dc.subject.meshAlcohol Oxidoreductases - Chemistry - Genetics - Metabolismen_US
dc.subject.meshArabidopsis - Cytology - Enzymology - Geneticsen_US
dc.subject.meshDna, Bacterial - Geneticsen_US
dc.subject.meshFertilityen_US
dc.subject.meshFlowers - Cytology - Enzymology - Genetics - Growth & Developmenten_US
dc.subject.meshGene Expression Regulation, Planten_US
dc.subject.meshGenetic Complementation Testen_US
dc.subject.meshGlucuronidase - Metabolismen_US
dc.subject.meshMutagenesis, Insertionalen_US
dc.subject.meshMutation - Geneticsen_US
dc.subject.meshOrgan Specificityen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProtein Transporten_US
dc.subject.meshSequence Analysis, Proteinen_US
dc.titleAn anther-specific dihydroflavonol 4-reductase-like gene (DRL1) is essential for male fertility in Arabidopsisen_US
dc.typeArticleen_US
dc.identifier.emailYip, WK: wkyip@hkucc.hku.hken_US
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_US
dc.identifier.authorityYip, WK=rp00833en_US
dc.identifier.authorityLo, C=rp00751en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1469-8137.2008.02692.xen_US
dc.identifier.pmid19054337-
dc.identifier.scopuseid_2-s2.0-58249105102en_US
dc.identifier.hkuros153377-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58249105102&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume181en_US
dc.identifier.issue3en_US
dc.identifier.spage576en_US
dc.identifier.epage587en_US
dc.identifier.eissn1469-8137-
dc.identifier.isiWOS:000262486500008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTang, LK=24605748900en_US
dc.identifier.scopusauthoridChu, H=36870371300en_US
dc.identifier.scopusauthoridYip, WK=7102784428en_US
dc.identifier.scopusauthoridYeung, EC=7101971768en_US
dc.identifier.scopusauthoridLo, C=15737175700en_US
dc.identifier.citeulike3917039-

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