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Article: Enhanced production of lutein in heterotrophic Chlorella protothecoides by oxidative stress

TitleEnhanced production of lutein in heterotrophic Chlorella protothecoides by oxidative stress
Authors
Issue Date2008
Publisher中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/
Citation
Science In China, Series C: Life Sciences, 2008, v. 51 n. 12, p. 1088-1093 How to Cite?
AbstractThe fast growing unicellular green microalgae Chlorella protothecoides has attracted interest as a promising organism for commercial production of a high-value carotenoid, lutein, by heterotrophic fermentation. Effects of two oxidant-forming reactive oxygen species (ROS) on the biomass concentration, and yield and content of lutein in batch culture of heterotrophic Chlorella protothecoides were investigated in this study. The addition of 0.1 mmol/L H 2O 2 and 0.01 mmol/L NaClO plus 0.5 mmol/L Fe 2+ to the culture led to the generation of •OH and enhanced the lutein content from 1.75 to 1.90 and 1.95 mg/g, respectively. The lutein content further increased to 1.98 mg/g when 0.01 mmol/L H 2O 2 and 0.5 mmol/L NaClO were added to generate 1O 2. The maximum yield of lutein (28.5, 29.8 and 31.4 mg/L) and a high biomass concentration (15.0, 15.3 and 15.9 g/L) were also achieved through the above treatments. The results indicated that 1O2 could promote lutein formation and enhance lutein production in heterotrophic Chlorella protothecoides. Moreover, 1O 2 produced from the reaction of H 2O 2 and NaClO was more effective in enhancing lutein production and reducing biomass loss than •OH from the reaction of H 2O 2 or NaClO plus Fe 2+. © 2008 Science in China Press and Springer-Verlag GmbH.
Persistent Identifierhttp://hdl.handle.net/10722/179110
ISSN
2011 Impact Factor: 1.61
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWei, Den_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorChen, Gen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorLiu, Len_US
dc.contributor.authorZhang, Hen_US
dc.date.accessioned2012-12-19T09:52:03Z-
dc.date.available2012-12-19T09:52:03Z-
dc.date.issued2008en_US
dc.identifier.citationScience In China, Series C: Life Sciences, 2008, v. 51 n. 12, p. 1088-1093en_US
dc.identifier.issn1006-9305en_US
dc.identifier.urihttp://hdl.handle.net/10722/179110-
dc.description.abstractThe fast growing unicellular green microalgae Chlorella protothecoides has attracted interest as a promising organism for commercial production of a high-value carotenoid, lutein, by heterotrophic fermentation. Effects of two oxidant-forming reactive oxygen species (ROS) on the biomass concentration, and yield and content of lutein in batch culture of heterotrophic Chlorella protothecoides were investigated in this study. The addition of 0.1 mmol/L H 2O 2 and 0.01 mmol/L NaClO plus 0.5 mmol/L Fe 2+ to the culture led to the generation of •OH and enhanced the lutein content from 1.75 to 1.90 and 1.95 mg/g, respectively. The lutein content further increased to 1.98 mg/g when 0.01 mmol/L H 2O 2 and 0.5 mmol/L NaClO were added to generate 1O 2. The maximum yield of lutein (28.5, 29.8 and 31.4 mg/L) and a high biomass concentration (15.0, 15.3 and 15.9 g/L) were also achieved through the above treatments. The results indicated that 1O2 could promote lutein formation and enhance lutein production in heterotrophic Chlorella protothecoides. Moreover, 1O 2 produced from the reaction of H 2O 2 and NaClO was more effective in enhancing lutein production and reducing biomass loss than •OH from the reaction of H 2O 2 or NaClO plus Fe 2+. © 2008 Science in China Press and Springer-Verlag GmbH.en_US
dc.languageengen_US
dc.publisher中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/en_US
dc.relation.ispartofScience in China, Series C: Life Sciencesen_US
dc.subject.meshAlgal Proteins - Metabolismen_US
dc.subject.meshChlorella - Chemistry - Metabolismen_US
dc.subject.meshHydrogen Peroxide - Metabolismen_US
dc.subject.meshLutein - Biosynthesis - Chemistryen_US
dc.subject.meshMolecular Structureen_US
dc.subject.meshOxidants - Metabolismen_US
dc.subject.meshOxidative Stressen_US
dc.subject.meshReactive Oxygen Species - Metabolismen_US
dc.subject.meshSodium Hypochlorite - Metabolismen_US
dc.titleEnhanced production of lutein in heterotrophic Chlorella protothecoides by oxidative stressen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11427-008-0145-2en_US
dc.identifier.pmid19093082-
dc.identifier.scopuseid_2-s2.0-58149104339en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58149104339&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume51en_US
dc.identifier.issue12en_US
dc.identifier.spage1088en_US
dc.identifier.epage1093en_US
dc.identifier.isiWOS:000261832500005-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWei, D=24472144100en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridChen, G=35309532100en_US
dc.identifier.scopusauthoridZhang, X=16044335800en_US
dc.identifier.scopusauthoridLiu, L=25930506800en_US
dc.identifier.scopusauthoridZhang, H=9238972800en_US
dc.identifier.citeulike3819453-

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