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Article: Sugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)

TitleSugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)
Authors
KeywordsΒ-Carotene Hydroxylase
Β-Carotene Ketolase
Astaxanthin
Chlorella Zofingiensis
Heterotrophic
Sugar
Issue Date2008
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 2008, v. 43 n. 11, p. 1288-1292 How to Cite?
AbstractThe green microalga Chlorella zofingiensis can grow and produce the ketocarotenoid astaxanthin in the dark with glucose as sole carbon and energy source. In the present study, we reported that glucose, mannose, fructose, sucrose, galactose and lactose could differentially support the cell growth and astaxanthin biosynthesis. Of the sugars surveyed, glucose and mannose were the best carbon sources for the algal growth in the dark, as indicated by the relatively high specific growth rates (ca. 0.03 h -1) and high cell dry densities (ca. 10 g l -1). Furthermore, the algal cells cultured with glucose and mannose accumulated the highest amounts of astaxanthin (ca. 1 mg g -1), indicating a correlation between cell growth and astaxanthin formation. In addition, various sugars differentially regulated the transcription of three carotenogenic genes encoding phytoene desaturase (PDS), β-carotene ketolase (BKT), and β-carotene hydroxylase (CHYb) respectively. By using glucose fed-batch fermentation, high cell dry weight concentration (ca. 53 g l -1) and high astaxanthin production (ca. 32 mg l -1) were obtained, which are much higher than those ever reported in the alga. The present study suggests that C. zofingiensis is suitable for the production of natural astaxanthin on a massive scale. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179088
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, Nen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorLi, YTen_US
dc.contributor.authorHuang, JCen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:51:52Z-
dc.date.available2012-12-19T09:51:52Z-
dc.date.issued2008en_US
dc.identifier.citationProcess Biochemistry, 2008, v. 43 n. 11, p. 1288-1292en_US
dc.identifier.issn1359-5113en_US
dc.identifier.urihttp://hdl.handle.net/10722/179088-
dc.description.abstractThe green microalga Chlorella zofingiensis can grow and produce the ketocarotenoid astaxanthin in the dark with glucose as sole carbon and energy source. In the present study, we reported that glucose, mannose, fructose, sucrose, galactose and lactose could differentially support the cell growth and astaxanthin biosynthesis. Of the sugars surveyed, glucose and mannose were the best carbon sources for the algal growth in the dark, as indicated by the relatively high specific growth rates (ca. 0.03 h -1) and high cell dry densities (ca. 10 g l -1). Furthermore, the algal cells cultured with glucose and mannose accumulated the highest amounts of astaxanthin (ca. 1 mg g -1), indicating a correlation between cell growth and astaxanthin formation. In addition, various sugars differentially regulated the transcription of three carotenogenic genes encoding phytoene desaturase (PDS), β-carotene ketolase (BKT), and β-carotene hydroxylase (CHYb) respectively. By using glucose fed-batch fermentation, high cell dry weight concentration (ca. 53 g l -1) and high astaxanthin production (ca. 32 mg l -1) were obtained, which are much higher than those ever reported in the alga. The present study suggests that C. zofingiensis is suitable for the production of natural astaxanthin on a massive scale. © 2008 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_US
dc.relation.ispartofProcess Biochemistryen_US
dc.subjectΒ-Carotene Hydroxylaseen_US
dc.subjectΒ-Carotene Ketolaseen_US
dc.subjectAstaxanthinen_US
dc.subjectChlorella Zofingiensisen_US
dc.subjectHeterotrophicen_US
dc.subjectSugaren_US
dc.titleSugar-based growth, astaxanthin accumulation and carotenogenic transcription of heterotrophic Chlorella zofingiensis (Chlorophyta)en_US
dc.typeArticleen_US
dc.identifier.emailHuang, JC: huangjc@hku.hken_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityHuang, JC=rp00710en_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.procbio.2008.07.014en_US
dc.identifier.scopuseid_2-s2.0-53049094204en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-53049094204&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume43en_US
dc.identifier.issue11en_US
dc.identifier.spage1288en_US
dc.identifier.epage1292en_US
dc.identifier.isiWOS:000260809300016-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSun, N=7202557210en_US
dc.identifier.scopusauthoridWang, Y=7601486161en_US
dc.identifier.scopusauthoridLi, YT=8875807300en_US
dc.identifier.scopusauthoridHuang, JC=7408108735en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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