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Article: Lipid characterization of Mortierella alpina grown at different NaCl concentrations

TitleLipid characterization of Mortierella alpina grown at different NaCl concentrations
Authors
Issue Date2008
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 2008, v. 56 n. 17, p. 7903-7909 How to Cite?
AbstractEffects of sodium chloride (NaCl) concentration on the lipid and fatty acid profiles of the polyunsaturated fatty acid (PUFA)-producing fungus, Mortierella alpina SC9, were investigated. The cells were cultivated in the medium with 4 different NaCl concentrations (0, 1, 2, 4%) for 6 days. The lipid and fatty acid profiles were analyzed by thin layer chromatography and gas chromatography. In the cultures with NaCl concentration up to 2%, PUFAs accounted for over 50% of the total fatty acids (TFAs) of the cells. Triacylglycerol (TAG) was the major lipid class, followed by monoacylglycerol (MAG) and diacylglycerol (DAG). TAG was found to contain the highest proportion of arachidonic acid (C20:4n-6, AA), suggesting that AA was mainly stored in the TAG. Comparing cultures at different NaCl concentrations indicated that TFA and TAG contents were higher in the cells grown at 2% NaCl. Similar results were found when 2% NaCl was added at day 3 of cultivation (late-log phase). In addition, the gene expression level of a TAG biosynthesis enzyme, diacylglycerol acyltransferase 2 (DGAT2), was also higher in the NaCl treated cells. This suggested that the increase of TFA and TAG contents might be related to the NaCl-stimulated DGAT2 expression. © 2008 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/179086
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, SYen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:51:51Z-
dc.date.available2012-12-19T09:51:51Z-
dc.date.issued2008en_US
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2008, v. 56 n. 17, p. 7903-7909en_US
dc.identifier.issn0021-8561en_US
dc.identifier.urihttp://hdl.handle.net/10722/179086-
dc.description.abstractEffects of sodium chloride (NaCl) concentration on the lipid and fatty acid profiles of the polyunsaturated fatty acid (PUFA)-producing fungus, Mortierella alpina SC9, were investigated. The cells were cultivated in the medium with 4 different NaCl concentrations (0, 1, 2, 4%) for 6 days. The lipid and fatty acid profiles were analyzed by thin layer chromatography and gas chromatography. In the cultures with NaCl concentration up to 2%, PUFAs accounted for over 50% of the total fatty acids (TFAs) of the cells. Triacylglycerol (TAG) was the major lipid class, followed by monoacylglycerol (MAG) and diacylglycerol (DAG). TAG was found to contain the highest proportion of arachidonic acid (C20:4n-6, AA), suggesting that AA was mainly stored in the TAG. Comparing cultures at different NaCl concentrations indicated that TFA and TAG contents were higher in the cells grown at 2% NaCl. Similar results were found when 2% NaCl was added at day 3 of cultivation (late-log phase). In addition, the gene expression level of a TAG biosynthesis enzyme, diacylglycerol acyltransferase 2 (DGAT2), was also higher in the NaCl treated cells. This suggested that the increase of TFA and TAG contents might be related to the NaCl-stimulated DGAT2 expression. © 2008 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_US
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_US
dc.subject.meshFatty Acids - Analysisen_US
dc.subject.meshFatty Acids, Unsaturated - Analysisen_US
dc.subject.meshLipids - Analysisen_US
dc.subject.meshMortierella - Chemistry - Growth & Developmenten_US
dc.subject.meshSodium Chloride - Administration & Dosageen_US
dc.subject.meshTriglycerides - Analysisen_US
dc.titleLipid characterization of Mortierella alpina grown at different NaCl concentrationsen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/jf801404yen_US
dc.identifier.pmid18683947-
dc.identifier.scopuseid_2-s2.0-52649144274en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-52649144274&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume56en_US
dc.identifier.issue17en_US
dc.identifier.spage7903en_US
dc.identifier.epage7909en_US
dc.identifier.isiWOS:000258938000048-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHo, SY=16426196000en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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