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Article: Effects of fifteen PBDE metabolites, DE71, DE79 and TBBPA on steroidogenesis in the H295R cell line

TitleEffects of fifteen PBDE metabolites, DE71, DE79 and TBBPA on steroidogenesis in the H295R cell line
Authors
Issue Date2008
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphere
Citation
Chemosphere, 2008, v. 71 n. 10, p. 1888-1894 How to Cite?
AbstractPolybrominated diphenyl ethers (PBDEs) and tetrabromobisphenol A (TBBPA) are brominated flame retardants that are produced in large quantities and are commonly used in construction materials, textiles, and as polymers in electronic equipment. Environmental and human levels of PBDEs have been increasing in the past 30 years, but the toxicity of PBDEs is not fully understood. Studies on their effects are relatively limited, and show that PBDEs are neurotoxins and potential endocrine disrupters. Hydroxylated (OH{single bond}) and methoxylated (MeO{single bond}) PBDEs have also been reported in the adipose tissue, blood and milk of wild animals and humans. In the present study, 15 PBDE metabolites, two BDE mixtures (DE71 and DE79), and TBBPA were studied individually to determine their effects on ten steroidogenic genes, aromatase activity, and concentrations of two steroid hormones (testosterone and 17β-estradiol) in the H295R human adrenocortical carcinoma cell line. Exposure to 0.05 μM 2′-OH-BDE-68 significantly induced the expression of CYP11A, CYP11B2, CYP17, CYP21, 3βHSD2, 17βHSD1, and 17βHSD4, and the expression of StAR was induced by 6-OH-BDE-90 at the three exposure concentrations. Exposure to DE71 and DE79 resulted in dose-dependent trend towards induction, but these effects were not significant. Exposure to 0.5 μM 2-OH-BDE-123 and 2-MeO-BDE-123 resulted in significantly greater aromatase activity. However, none of the compounds affected sex hormone production at the concentrations tested. Generally, OH-BDEs had a much stronger ability to affect steroidogenic gene expression than MeO-BDEs. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179050
ISSN
2015 Impact Factor: 3.698
2015 SCImago Journal Rankings: 1.536
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSong, Ren_US
dc.contributor.authorHe, Yen_US
dc.contributor.authorMurphy, MBen_US
dc.contributor.authorYeung, LWYen_US
dc.contributor.authorYu, RMKen_US
dc.contributor.authorLam, MHWen_US
dc.contributor.authorLam, PKSen_US
dc.contributor.authorHecker, Men_US
dc.contributor.authorGiesy, JPen_US
dc.contributor.authorWu, RSSen_US
dc.contributor.authorZhang, Wen_US
dc.contributor.authorSheng, Gen_US
dc.contributor.authorFu, Jen_US
dc.date.accessioned2012-12-19T09:51:39Z-
dc.date.available2012-12-19T09:51:39Z-
dc.date.issued2008en_US
dc.identifier.citationChemosphere, 2008, v. 71 n. 10, p. 1888-1894en_US
dc.identifier.issn0045-6535en_US
dc.identifier.urihttp://hdl.handle.net/10722/179050-
dc.description.abstractPolybrominated diphenyl ethers (PBDEs) and tetrabromobisphenol A (TBBPA) are brominated flame retardants that are produced in large quantities and are commonly used in construction materials, textiles, and as polymers in electronic equipment. Environmental and human levels of PBDEs have been increasing in the past 30 years, but the toxicity of PBDEs is not fully understood. Studies on their effects are relatively limited, and show that PBDEs are neurotoxins and potential endocrine disrupters. Hydroxylated (OH{single bond}) and methoxylated (MeO{single bond}) PBDEs have also been reported in the adipose tissue, blood and milk of wild animals and humans. In the present study, 15 PBDE metabolites, two BDE mixtures (DE71 and DE79), and TBBPA were studied individually to determine their effects on ten steroidogenic genes, aromatase activity, and concentrations of two steroid hormones (testosterone and 17β-estradiol) in the H295R human adrenocortical carcinoma cell line. Exposure to 0.05 μM 2′-OH-BDE-68 significantly induced the expression of CYP11A, CYP11B2, CYP17, CYP21, 3βHSD2, 17βHSD1, and 17βHSD4, and the expression of StAR was induced by 6-OH-BDE-90 at the three exposure concentrations. Exposure to DE71 and DE79 resulted in dose-dependent trend towards induction, but these effects were not significant. Exposure to 0.5 μM 2-OH-BDE-123 and 2-MeO-BDE-123 resulted in significantly greater aromatase activity. However, none of the compounds affected sex hormone production at the concentrations tested. Generally, OH-BDEs had a much stronger ability to affect steroidogenic gene expression than MeO-BDEs. © 2008 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/chemosphereen_US
dc.relation.ispartofChemosphereen_US
dc.subject.meshAromatase - Metabolismen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCytochrome P-450 Enzyme System - Geneticsen_US
dc.subject.meshEstradiol - Metabolismen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshHydroxysteroid Dehydrogenases - Geneticsen_US
dc.subject.meshPhenyl Ethers - Toxicityen_US
dc.subject.meshPhosphoproteins - Geneticsen_US
dc.subject.meshPolybrominated Biphenyls - Toxicityen_US
dc.subject.meshTestosterone - Metabolismen_US
dc.titleEffects of fifteen PBDE metabolites, DE71, DE79 and TBBPA on steroidogenesis in the H295R cell lineen_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.chemosphere.2008.01.032en_US
dc.identifier.pmid18313098-
dc.identifier.scopuseid_2-s2.0-42249092646en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-42249092646&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume71en_US
dc.identifier.issue10en_US
dc.identifier.spage1888en_US
dc.identifier.epage1894en_US
dc.identifier.isiWOS:000256257000013-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSong, R=8632448500en_US
dc.identifier.scopusauthoridHe, Y=16241582000en_US
dc.identifier.scopusauthoridMurphy, MB=7403900446en_US
dc.identifier.scopusauthoridYeung, LWY=9735175200en_US
dc.identifier.scopusauthoridYu, RMK=9278574900en_US
dc.identifier.scopusauthoridLam, MHW=7202630175en_US
dc.identifier.scopusauthoridLam, PKS=7202365776en_US
dc.identifier.scopusauthoridHecker, M=35247848500en_US
dc.identifier.scopusauthoridGiesy, JP=35459135300en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US
dc.identifier.scopusauthoridZhang, W=8235376700en_US
dc.identifier.scopusauthoridSheng, G=7103200508en_US
dc.identifier.scopusauthoridFu, J=7401722414en_US
dc.identifier.citeulike9570973-

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