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Article: Antitumor activity of 3,5,4′-trimethoxystilbene in COLO 205 cells and xenografts in SCID mice

TitleAntitumor activity of 3,5,4′-trimethoxystilbene in COLO 205 cells and xenografts in SCID mice
Authors
KeywordsApoptosis
Caspase-3
Caspase-9
Cytochrome-C
Mr-3
P53
Scid Mice
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/
Citation
Molecular Carcinogenesis, 2008, v. 47 n. 3, p. 184-196 How to Cite?
AbstractResveratrol (R-3), a trihydroxy trans-stilbene from grape, inhibits multistage carcinogenesis in animal models. Here we report that 3,5,4′-thmethoxystilbene (MR-3), the permethylated derivative of R-3 was more potent against the growth of human cancer cells (HT-29, PC-3, COLO 205) with estimated IC 50 values of 81.31,42.71, and 6.25 μM, respectively. We further observed that MR-3 induced apoptosis in COLO 205 cells through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). ROS generation occurs in the early stages of MR-3-induced apoptosis, preceding cytochrome-c release, caspase activation, and DNA fragmentation. Significant therapeutic effects were demonstrated in vivo by treating severe combined immune deficiency (SCID) mice bearing COLO 205 tumor xenografts with MR-3 (50 mg/kg ip). Assays on DNA fragmentation and caspase activation were performed and demonstrated that apoptosis occurred in tumor tissues treated with MR-3. The appearance of apoptotic cells, as shown by Hematoxylin and Eosin (H&E) staining, and an increase in p21 and decrease in proliferating cell nuclear antigen (PCNA) protein by immuno-histochemistry were observed in tumor tissues under MR-3 treatment. Our study identifies the novel mechanisms of the antitumor effects of MR-3 and indicates that these results may have significant applications for cancer chemotherapy. © 2007 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/179044
ISSN
2015 Impact Factor: 4.722
2015 SCImago Journal Rankings: 1.393
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPan, MHen_US
dc.contributor.authorGao, JHen_US
dc.contributor.authorLai, CSen_US
dc.contributor.authorWang, YJen_US
dc.contributor.authorChen, WMen_US
dc.contributor.authorLo, CYen_US
dc.contributor.authorWang, Men_US
dc.contributor.authorDushenkov, Sen_US
dc.contributor.authorHo, CTen_US
dc.date.accessioned2012-12-19T09:51:37Z-
dc.date.available2012-12-19T09:51:37Z-
dc.date.issued2008en_US
dc.identifier.citationMolecular Carcinogenesis, 2008, v. 47 n. 3, p. 184-196en_US
dc.identifier.issn0899-1987en_US
dc.identifier.urihttp://hdl.handle.net/10722/179044-
dc.description.abstractResveratrol (R-3), a trihydroxy trans-stilbene from grape, inhibits multistage carcinogenesis in animal models. Here we report that 3,5,4′-thmethoxystilbene (MR-3), the permethylated derivative of R-3 was more potent against the growth of human cancer cells (HT-29, PC-3, COLO 205) with estimated IC 50 values of 81.31,42.71, and 6.25 μM, respectively. We further observed that MR-3 induced apoptosis in COLO 205 cells through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). ROS generation occurs in the early stages of MR-3-induced apoptosis, preceding cytochrome-c release, caspase activation, and DNA fragmentation. Significant therapeutic effects were demonstrated in vivo by treating severe combined immune deficiency (SCID) mice bearing COLO 205 tumor xenografts with MR-3 (50 mg/kg ip). Assays on DNA fragmentation and caspase activation were performed and demonstrated that apoptosis occurred in tumor tissues treated with MR-3. The appearance of apoptotic cells, as shown by Hematoxylin and Eosin (H&E) staining, and an increase in p21 and decrease in proliferating cell nuclear antigen (PCNA) protein by immuno-histochemistry were observed in tumor tissues under MR-3 treatment. Our study identifies the novel mechanisms of the antitumor effects of MR-3 and indicates that these results may have significant applications for cancer chemotherapy. © 2007 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0899-1987/en_US
dc.relation.ispartofMolecular Carcinogenesisen_US
dc.rightsMolecular Carcinogenesis. Copyright © John Wiley & Sons, Inc.-
dc.subjectApoptosisen_US
dc.subjectCaspase-3en_US
dc.subjectCaspase-9en_US
dc.subjectCytochrome-Cen_US
dc.subjectMr-3en_US
dc.subjectP53en_US
dc.subjectScid Miceen_US
dc.titleAntitumor activity of 3,5,4′-trimethoxystilbene in COLO 205 cells and xenografts in SCID miceen_US
dc.typeArticleen_US
dc.identifier.emailWang, M: mfwang@hku.hken_US
dc.identifier.authorityWang, M=rp00800en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/mc.20352en_US
dc.identifier.pmid18085528-
dc.identifier.scopuseid_2-s2.0-40749160084en_US
dc.identifier.hkuros142347-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40749160084&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume47en_US
dc.identifier.issue3en_US
dc.identifier.spage184en_US
dc.identifier.epage196en_US
dc.identifier.isiWOS:000254260300003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPan, MH=7202544934en_US
dc.identifier.scopusauthoridGao, JH=35083413100en_US
dc.identifier.scopusauthoridLai, CS=8744893900en_US
dc.identifier.scopusauthoridWang, YJ=35081383400en_US
dc.identifier.scopusauthoridChen, WM=35228681300en_US
dc.identifier.scopusauthoridLo, CY=8384768400en_US
dc.identifier.scopusauthoridWang, M=7406691844en_US
dc.identifier.scopusauthoridDushenkov, S=6603390833en_US
dc.identifier.scopusauthoridHo, CT=7404652573en_US

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