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Article: Cloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissues

TitleCloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissues
Authors
Issue Date2007
PublisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.org
Citation
Poultry Science, 2007, v. 86 n. 2, p. 423-430 How to Cite?
AbstractSubstantial evidence suggests that glucocorticoids play critical roles in the differentiation of somatotroph and lactotroph in embryonic pituitaries of birds. However, the basic information on the expression of glucocorticoid receptor (GR) in avian species is limited. In this study, the full-length cDNA for chicken GR was cloned from the chicken kidney. It encodes 772 amino acids and shares high homology with that of the human (73%), mouse (73%), rat (71%), rabbit (72%), and trout (51%) sequences. Similar to mammals, chicken GR is widely expressed in all adult tissues being investigated. Among the 12 tissues investigated, relatively high expression of GR was detected in pituitary, muscle, ovary, and kidney using reverse transcription-PCR assay. Using semiquantitative reverse transcription-PCR, GR is shown to be abundantly expressed at a more or less constant level during embryonic pituitary development (from d 8 to 20), supporting the hypothesis that the expression of GR is unlikely to be a limiting factor in initiating the differentiation of somatotroph and lactotroph in embryonic pituitary of birds. Moreover, an abundant expression of GR in the whole embryos at earlier developmental stages (from d 2 to 5) was also detected in the present study, though its physiological relevance remains to be determined. ©2007 Poultry Science Association Inc.
Persistent Identifierhttp://hdl.handle.net/10722/178986
ISSN
2015 Impact Factor: 1.685
2015 SCImago Journal Rankings: 1.162
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKwok, AHYen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorWang, CYen_US
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:51:15Z-
dc.date.available2012-12-19T09:51:15Z-
dc.date.issued2007en_US
dc.identifier.citationPoultry Science, 2007, v. 86 n. 2, p. 423-430en_US
dc.identifier.issn0032-5791en_US
dc.identifier.urihttp://hdl.handle.net/10722/178986-
dc.description.abstractSubstantial evidence suggests that glucocorticoids play critical roles in the differentiation of somatotroph and lactotroph in embryonic pituitaries of birds. However, the basic information on the expression of glucocorticoid receptor (GR) in avian species is limited. In this study, the full-length cDNA for chicken GR was cloned from the chicken kidney. It encodes 772 amino acids and shares high homology with that of the human (73%), mouse (73%), rat (71%), rabbit (72%), and trout (51%) sequences. Similar to mammals, chicken GR is widely expressed in all adult tissues being investigated. Among the 12 tissues investigated, relatively high expression of GR was detected in pituitary, muscle, ovary, and kidney using reverse transcription-PCR assay. Using semiquantitative reverse transcription-PCR, GR is shown to be abundantly expressed at a more or less constant level during embryonic pituitary development (from d 8 to 20), supporting the hypothesis that the expression of GR is unlikely to be a limiting factor in initiating the differentiation of somatotroph and lactotroph in embryonic pituitary of birds. Moreover, an abundant expression of GR in the whole embryos at earlier developmental stages (from d 2 to 5) was also detected in the present study, though its physiological relevance remains to be determined. ©2007 Poultry Science Association Inc.en_US
dc.languageengen_US
dc.publisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.orgen_US
dc.relation.ispartofPoultry Scienceen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshChickens - Geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshKidney - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMuscle, Skeletal - Metabolismen_US
dc.subject.meshOvary - Metabolismen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshPituitary Gland - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshReceptors, Glucocorticoid - Chemistry - Geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleCloning of chicken glucocorticoid receptor (GR) and characterization of its expression in pituitary and extrapituitary tissuesen_US
dc.typeArticleen_US
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityWang, Y=rp00801en_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/ps/86.2.423-
dc.identifier.pmid17234861-
dc.identifier.scopuseid_2-s2.0-33947509354en_US
dc.identifier.hkuros133446-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947509354&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume86en_US
dc.identifier.issue2en_US
dc.identifier.spage423en_US
dc.identifier.epage430en_US
dc.identifier.isiWOS:000243841800031-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKwok, AHY=27168105100en_US
dc.identifier.scopusauthoridWang, Y=36062525200en_US
dc.identifier.scopusauthoridWang, CY=16065173000en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US

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