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Article: Coexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacterium

TitleCoexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacterium
Authors
KeywordsBiodegradation
Carboxylesterase (Cbe)
Coexpression
Organophosphate Hydrolase (Oph)
Issue Date2006
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
Citation
International Biodeterioration And Biodegradation, 2006, v. 58 n. 2, p. 70-76 How to Cite?
AbstractDegradation of pesticides is usually beneficial, since the reactions that destroy pesticides convert most pesticide residues in the environment to inactive, less toxic, harmless compounds. This paper describes a novel strategy using a coexpression vector for the purpose of developing bacteria that can detoxify different pesticides. The vector pETDuet was designed for coexpression of two target genes simultaneously. The organophosphate hydrolase gene (opd) from Flavobacterium sp. and carboxylesterase B1 gene (b1) from Culex pipiens were cloned in the coexpression vector. The expressed enzymes of OPH and B1 had a molecular mass of approximately 35 and 65 kDa, respectively. A single microorganism was capable of producing both enzymes for degradation of organophosphorus, carbamate and pyrethroid pesticides. The technical capability of genetically engineering bacteria with more enzymes should open up new opportunities for extending the wide range of pesticides that can be biodegraded in the future. © 2006 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178965
ISSN
2015 Impact Factor: 2.429
2015 SCImago Journal Rankings: 0.919
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLan, WSen_US
dc.contributor.authorGu, JDen_US
dc.contributor.authorZhang, JLen_US
dc.contributor.authorShen, BCen_US
dc.contributor.authorJiang, Hen_US
dc.contributor.authorMulchandani, Aen_US
dc.contributor.authorChen, Wen_US
dc.contributor.authorQiao, CLen_US
dc.date.accessioned2012-12-19T09:51:07Z-
dc.date.available2012-12-19T09:51:07Z-
dc.date.issued2006en_US
dc.identifier.citationInternational Biodeterioration And Biodegradation, 2006, v. 58 n. 2, p. 70-76en_US
dc.identifier.issn0964-8305en_US
dc.identifier.urihttp://hdl.handle.net/10722/178965-
dc.description.abstractDegradation of pesticides is usually beneficial, since the reactions that destroy pesticides convert most pesticide residues in the environment to inactive, less toxic, harmless compounds. This paper describes a novel strategy using a coexpression vector for the purpose of developing bacteria that can detoxify different pesticides. The vector pETDuet was designed for coexpression of two target genes simultaneously. The organophosphate hydrolase gene (opd) from Flavobacterium sp. and carboxylesterase B1 gene (b1) from Culex pipiens were cloned in the coexpression vector. The expressed enzymes of OPH and B1 had a molecular mass of approximately 35 and 65 kDa, respectively. A single microorganism was capable of producing both enzymes for degradation of organophosphorus, carbamate and pyrethroid pesticides. The technical capability of genetically engineering bacteria with more enzymes should open up new opportunities for extending the wide range of pesticides that can be biodegraded in the future. © 2006 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibioden_US
dc.relation.ispartofInternational Biodeterioration and Biodegradationen_US
dc.subjectBiodegradationen_US
dc.subjectCarboxylesterase (Cbe)en_US
dc.subjectCoexpressionen_US
dc.subjectOrganophosphate Hydrolase (Oph)en_US
dc.titleCoexpression of two detoxifying pesticide-degrading enzymes in a genetically engineered bacteriumen_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ibiod.2006.07.008en_US
dc.identifier.scopuseid_2-s2.0-33750323503en_US
dc.identifier.hkuros134270-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750323503&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume58en_US
dc.identifier.issue2en_US
dc.identifier.spage70en_US
dc.identifier.epage76en_US
dc.identifier.isiWOS:000242779600004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLan, WS=8935169200en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US
dc.identifier.scopusauthoridZhang, JL=35217451000en_US
dc.identifier.scopusauthoridShen, BC=24438411000en_US
dc.identifier.scopusauthoridJiang, H=34769714800en_US
dc.identifier.scopusauthoridMulchandani, A=7006356390en_US
dc.identifier.scopusauthoridChen, W=7409646857en_US
dc.identifier.scopusauthoridQiao, CL=7101795699en_US

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