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Article: Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs

TitleBiodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gs
Authors
Keywords1-Methylindole
3-Methylindole
Degradation
First-Order Kinetic Model
Mangrove Sediment
Pseudomonas Aeruginosa Gs
Issue Date2006
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0049-6979
Citation
Water, Air, And Soil Pollution, 2006, v. 176 n. 1-4, p. 185-199 How to Cite?
AbstractIndolic compounds are N-heterocyclic aromatic chemicals and have been detected at contaminated sites. Biodegradation of 1-methylindole (1MI) and 3-methylindole (3MI) was investigated initially using enrichment cultures with mangrove sediment obtained from Mai Po Nature Reserve of Hong Kong and subsequently with a pure culture of Pseudomonas aeruginosa Gs confirmed with 16S rRNA gene. At 2.0 mM, 1MI and 3MI were degraded in 4 and 3 days, respectively, by the respective 1MI- and 3MI-degrading enrichment cultures. When substrate concentrations were increased to 3.0,mM and 3.5,mM, slower degradation of 1MI and 3MI was observed indicating inhibitory effects from the substrates, possibly due to toxicity. In addition, no colony of bacteria could be observed on the agar plates amended with 3.5,mM 1MI or 4.0,mM 3MI, indicating that 1MI was more toxic than 3MI. Pseudomonas aeruginosa Gs, isolated from the enrichment culture, effectively utilized both substrates as the sole source of carbon and energy. Complete degradation of 1MI and 3MI was achieved after more than 40 days and 24 days, respectively, at an initial concentration of 2,mM in the culture. Effects of initial substrate concentration, pH and salinity on degradation of 1MI and 3MI by P. aeruginosa Gs were also studied in batch culture. The optimum pH and salinity for degrading both substrates by P. aeruginosa Gs was 7.0 and 5‰, respectively. Biodegradation kinetics of 1MI and 3MI by P. aeruginosa Gs could be described using a first-order kinetic model. Our results suggest that both 1MI and 3MI are biodegradable in the mangrove environment and that toxicity of 1MI could be a potential factor limiting the removal of the chemical in the environment by microorganisms. © Springer 2006.
Persistent Identifierhttp://hdl.handle.net/10722/178954
ISSN
2015 Impact Factor: 1.551
2015 SCImago Journal Rankings: 0.632
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYin, Ben_US
dc.contributor.authorHuang, Len_US
dc.contributor.authorGu, JDen_US
dc.date.accessioned2012-12-19T09:51:01Z-
dc.date.available2012-12-19T09:51:01Z-
dc.date.issued2006en_US
dc.identifier.citationWater, Air, And Soil Pollution, 2006, v. 176 n. 1-4, p. 185-199en_US
dc.identifier.issn0049-6979en_US
dc.identifier.urihttp://hdl.handle.net/10722/178954-
dc.description.abstractIndolic compounds are N-heterocyclic aromatic chemicals and have been detected at contaminated sites. Biodegradation of 1-methylindole (1MI) and 3-methylindole (3MI) was investigated initially using enrichment cultures with mangrove sediment obtained from Mai Po Nature Reserve of Hong Kong and subsequently with a pure culture of Pseudomonas aeruginosa Gs confirmed with 16S rRNA gene. At 2.0 mM, 1MI and 3MI were degraded in 4 and 3 days, respectively, by the respective 1MI- and 3MI-degrading enrichment cultures. When substrate concentrations were increased to 3.0,mM and 3.5,mM, slower degradation of 1MI and 3MI was observed indicating inhibitory effects from the substrates, possibly due to toxicity. In addition, no colony of bacteria could be observed on the agar plates amended with 3.5,mM 1MI or 4.0,mM 3MI, indicating that 1MI was more toxic than 3MI. Pseudomonas aeruginosa Gs, isolated from the enrichment culture, effectively utilized both substrates as the sole source of carbon and energy. Complete degradation of 1MI and 3MI was achieved after more than 40 days and 24 days, respectively, at an initial concentration of 2,mM in the culture. Effects of initial substrate concentration, pH and salinity on degradation of 1MI and 3MI by P. aeruginosa Gs were also studied in batch culture. The optimum pH and salinity for degrading both substrates by P. aeruginosa Gs was 7.0 and 5‰, respectively. Biodegradation kinetics of 1MI and 3MI by P. aeruginosa Gs could be described using a first-order kinetic model. Our results suggest that both 1MI and 3MI are biodegradable in the mangrove environment and that toxicity of 1MI could be a potential factor limiting the removal of the chemical in the environment by microorganisms. © Springer 2006.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0049-6979en_US
dc.relation.ispartofWater, Air, and Soil Pollutionen_US
dc.subject1-Methylindoleen_US
dc.subject3-Methylindoleen_US
dc.subjectDegradationen_US
dc.subjectFirst-Order Kinetic Modelen_US
dc.subjectMangrove Sedimenten_US
dc.subjectPseudomonas Aeruginosa Gsen_US
dc.titleBiodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosa Gsen_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11270-006-9159-1en_US
dc.identifier.scopuseid_2-s2.0-33748459210en_US
dc.identifier.hkuros134300-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748459210&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume176en_US
dc.identifier.issue1-4en_US
dc.identifier.spage185en_US
dc.identifier.epage199en_US
dc.identifier.isiWOS:000240318000012-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridYin, B=36085664700en_US
dc.identifier.scopusauthoridHuang, L=7404736283en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US
dc.identifier.citeulike862141-

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