File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Expression profiles of growth hormone-releasing hormone and growth hormone-releasing hormone receptor during chicken embryonic pituitary development

TitleExpression profiles of growth hormone-releasing hormone and growth hormone-releasing hormone receptor during chicken embryonic pituitary development
Authors
Issue Date2006
PublisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.org
Citation
Poultry Science, 2006, v. 85 n. 3, p. 569-576 How to Cite?
AbstractGrowth hormone-releasing hormone (GHRH) and its receptor (GHRHR) have long been regarded as the critical molecules for the stimulation of growth hormone (GH) synthesis and release, as well as the regulation of pituitary somatotroph expansion in vertebrates. However, little is known about their expression in the embryonic pituitaries of birds. In this study, the full-length cDNA for chicken GHRHR was cloned from the chicken pituitary. It encodes 419 amino acids and shares high homology with that of the human, rat, and mouse. As in those in mammals, chicken GHRHR is predominantly expressed in the pituitary and weakly expressed in several extra-pituitary tissues including brain, pancreas, testis, and kidney, among 12 tissues examined. Using semiquantitative reverse transcription-PCR, we further examined the expression of GH, GHRH, and GHRHR during embryonic pituitary development. The expression of GHRHR on embryonic d 8 was much lower, but abundant expression was noticed as early as embryonic d 12. In contrast, the level of pituitary GHRH mRNA peaked on d 8 and declined sharply afterwards. Interestingly, unlike those of pituitary GHRH and GHRHR, the higher expression levels ofGHappeared much later (from d 16 to 20). The differential expressions of GHRH, GHRHR, andGH in the developing embryonic pituitaries not only imply that pituitary-derived GHRH (or pituitary adenylate cyclase-activating polypeptide) and GHRHR may have a paracrine/autocrine role in the expansion of undifferentiated somatotroph precursor cells, but also suggest that GHRHR is likely to be involved in the somatotroph differentiation occurring at the later developmental stages. ©2006 Poultry Science Association, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/178939
ISSN
2015 Impact Factor: 1.685
2015 SCImago Journal Rankings: 1.162
References

 

DC FieldValueLanguage
dc.contributor.authorWang, CYen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorLi, Jen_US
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:50:52Z-
dc.date.available2012-12-19T09:50:52Z-
dc.date.issued2006en_US
dc.identifier.citationPoultry Science, 2006, v. 85 n. 3, p. 569-576en_US
dc.identifier.issn0032-5791en_US
dc.identifier.urihttp://hdl.handle.net/10722/178939-
dc.description.abstractGrowth hormone-releasing hormone (GHRH) and its receptor (GHRHR) have long been regarded as the critical molecules for the stimulation of growth hormone (GH) synthesis and release, as well as the regulation of pituitary somatotroph expansion in vertebrates. However, little is known about their expression in the embryonic pituitaries of birds. In this study, the full-length cDNA for chicken GHRHR was cloned from the chicken pituitary. It encodes 419 amino acids and shares high homology with that of the human, rat, and mouse. As in those in mammals, chicken GHRHR is predominantly expressed in the pituitary and weakly expressed in several extra-pituitary tissues including brain, pancreas, testis, and kidney, among 12 tissues examined. Using semiquantitative reverse transcription-PCR, we further examined the expression of GH, GHRH, and GHRHR during embryonic pituitary development. The expression of GHRHR on embryonic d 8 was much lower, but abundant expression was noticed as early as embryonic d 12. In contrast, the level of pituitary GHRH mRNA peaked on d 8 and declined sharply afterwards. Interestingly, unlike those of pituitary GHRH and GHRHR, the higher expression levels ofGHappeared much later (from d 16 to 20). The differential expressions of GHRH, GHRHR, andGH in the developing embryonic pituitaries not only imply that pituitary-derived GHRH (or pituitary adenylate cyclase-activating polypeptide) and GHRHR may have a paracrine/autocrine role in the expansion of undifferentiated somatotroph precursor cells, but also suggest that GHRHR is likely to be involved in the somatotroph differentiation occurring at the later developmental stages. ©2006 Poultry Science Association, Inc.en_US
dc.languageengen_US
dc.publisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.orgen_US
dc.relation.ispartofPoultry Scienceen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChick Embryo - Metabolismen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshGrowth Hormone-Releasing Hormone - Genetics - Metabolismen_US
dc.subject.meshPituitary Gland - Embryology - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshReceptors, Neuropeptide - Chemistry - Genetics - Metabolismen_US
dc.subject.meshReceptors, Pituitary Hormone-Regulating Hormone - Chemistry - Genetics - Metabolismen_US
dc.titleExpression profiles of growth hormone-releasing hormone and growth hormone-releasing hormone receptor during chicken embryonic pituitary developmenten_US
dc.typeArticleen_US
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityWang, Y=rp00801en_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1093/ps/85.3.569-
dc.identifier.pmid16553291-
dc.identifier.scopuseid_2-s2.0-33646926994en_US
dc.identifier.hkuros122949-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646926994&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume85en_US
dc.identifier.issue3en_US
dc.identifier.spage569en_US
dc.identifier.epage576en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWang, CY=16065173000en_US
dc.identifier.scopusauthoridWang, Y=36062525200en_US
dc.identifier.scopusauthoridLi, J=27171035300en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats