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Article: Simultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCR

TitleSimultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCR
Authors
Issue Date2006
PublisherEaton Publishing Co. The Journal's web site is located at http://www.biotechniques.com
Citation
Biotechniques, 2006, v. 40 n. 2, p. 187-189 How to Cite?
AbstractA new approach for simultaneously amplifying the 5′ and 3′ ends of a desired cDNA is described. The method combines the template-switching effect with inverse PCR, which generates the flanking 5′ and 3′ regions of a certain cDNA with very low or no background. It requires only minimal amounts of total RNA for the synthesis of first-strand cDNA, while the same cDNA can be used to amplify flanking sequences of any cDNA species present in the sample. This method is reliable and easy to perform, which is very useful for isolating cDNA species of rare transcripts.
Persistent Identifierhttp://hdl.handle.net/10722/178935
ISSN
2015 Impact Factor: 2.298
2015 SCImago Journal Rankings: 1.132
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, JCen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:50:51Z-
dc.date.available2012-12-19T09:50:51Z-
dc.date.issued2006en_US
dc.identifier.citationBiotechniques, 2006, v. 40 n. 2, p. 187-189en_US
dc.identifier.issn0736-6205en_US
dc.identifier.urihttp://hdl.handle.net/10722/178935-
dc.description.abstractA new approach for simultaneously amplifying the 5′ and 3′ ends of a desired cDNA is described. The method combines the template-switching effect with inverse PCR, which generates the flanking 5′ and 3′ regions of a certain cDNA with very low or no background. It requires only minimal amounts of total RNA for the synthesis of first-strand cDNA, while the same cDNA can be used to amplify flanking sequences of any cDNA species present in the sample. This method is reliable and easy to perform, which is very useful for isolating cDNA species of rare transcripts.en_US
dc.languageengen_US
dc.publisherEaton Publishing Co. The Journal's web site is located at http://www.biotechniques.comen_US
dc.relation.ispartofBioTechniquesen_US
dc.titleSimultaneous amplification of 5' and 3' cDNA ends based on template-switching effect and inverse PCRen_US
dc.typeArticleen_US
dc.identifier.emailHuang, JC: huangjc@hku.hken_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityHuang, JC=rp00710en_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2144/000112051en_US
dc.identifier.pmid16526408-
dc.identifier.scopuseid_2-s2.0-33645513978en_US
dc.identifier.hkuros122423-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645513978&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage187en_US
dc.identifier.epage189en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHuang, JC=7408108735en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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