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Article: Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) as a Growth Hormone (GH)-releasing factor in grass carp. I. Functional coupling of cyclic adenosine 3′,5′-monophosphate and ca 2+/calmodulin- dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells

TitlePituitary Adenylate Cyclase-Activating Polypeptide (PACAP) as a Growth Hormone (GH)-releasing factor in grass carp. I. Functional coupling of cyclic adenosine 3′,5′-monophosphate and ca 2+/calmodulin- dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells
Authors
Issue Date2005
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2005, v. 146 n. 12, p. 5407-5424 How to Cite?
AbstractPituitary adenylate cyclase-activating polypeptide (PACAP), a member of the glucagon/secretin peptide family, has been recently proposed to be the ancestral GH-releasing factor. Using grass carp as a model for bony fish, we examined the mechanisms for PACAP regulation of GH synthesis and secretion at the pituitary level. Nerve fibers with PACAP immunoreactivity were identified in the grass carp pituitary overlapping with the distribution of somatotrophs. At the somatotroph level, PACAP was shown to induce cAMP synthesis and Ca 2+ entry through voltage-sensitive Ca 2+ channels (VSCC). In carp pituitary cells, PACAP but not vasoactive intestinal polypeptide increased GH release, GH content, total GH production, and steady-state GH mRNA levels. PACAP also enhanced GH mRNA stability, GH promoter activity, and nuclear expression of GH primary transcripts. Increasing cAMP levels, induction of Ca 2+ entry, and activation of VSCC were all effective in elevating GH secretion and GH mRNA levels. PACAP-induced GH secretion and GH mRNA expression, however, were abolished by inhibiting adenylate cyclase and protein kinase A, removing extracellular Ca 2+ or VSCC blockade, or inactivating calmodulin (CaM)-dependent protein kinase II (CaM kinase II). Similar sensitivity to VSCC and CaM kinase II blockade was also observed by activating cAMP production as a trigger for GH release and GH gene expression. These results suggest that PACAP stimulates GH synthesis and secretion in grass carp pituitary cells through PAC 1 receptors. These stimulatory actions probably are mediated by the adenylate cyclase/cAMP/protein kinase A pathway coupled to Ca 2+ entry via VSCC and subsequent activation of CaM/CaM kinase II cascades. Copyright © 2005 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/178913
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, AOLen_US
dc.contributor.authorLi, Wen_US
dc.contributor.authorLeung, CYen_US
dc.contributor.authorHuo, Len_US
dc.contributor.authorZhou, Hen_US
dc.date.accessioned2012-12-19T09:50:41Z-
dc.date.available2012-12-19T09:50:41Z-
dc.date.issued2005en_US
dc.identifier.citationEndocrinology, 2005, v. 146 n. 12, p. 5407-5424en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/178913-
dc.description.abstractPituitary adenylate cyclase-activating polypeptide (PACAP), a member of the glucagon/secretin peptide family, has been recently proposed to be the ancestral GH-releasing factor. Using grass carp as a model for bony fish, we examined the mechanisms for PACAP regulation of GH synthesis and secretion at the pituitary level. Nerve fibers with PACAP immunoreactivity were identified in the grass carp pituitary overlapping with the distribution of somatotrophs. At the somatotroph level, PACAP was shown to induce cAMP synthesis and Ca 2+ entry through voltage-sensitive Ca 2+ channels (VSCC). In carp pituitary cells, PACAP but not vasoactive intestinal polypeptide increased GH release, GH content, total GH production, and steady-state GH mRNA levels. PACAP also enhanced GH mRNA stability, GH promoter activity, and nuclear expression of GH primary transcripts. Increasing cAMP levels, induction of Ca 2+ entry, and activation of VSCC were all effective in elevating GH secretion and GH mRNA levels. PACAP-induced GH secretion and GH mRNA expression, however, were abolished by inhibiting adenylate cyclase and protein kinase A, removing extracellular Ca 2+ or VSCC blockade, or inactivating calmodulin (CaM)-dependent protein kinase II (CaM kinase II). Similar sensitivity to VSCC and CaM kinase II blockade was also observed by activating cAMP production as a trigger for GH release and GH gene expression. These results suggest that PACAP stimulates GH synthesis and secretion in grass carp pituitary cells through PAC 1 receptors. These stimulatory actions probably are mediated by the adenylate cyclase/cAMP/protein kinase A pathway coupled to Ca 2+ entry via VSCC and subsequent activation of CaM/CaM kinase II cascades. Copyright © 2005 by The Endocrine Society.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.rightsEndocrinology. Copyright © The Endocrine Society.-
dc.subject.meshAnimalsen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinases - Metabolismen_US
dc.subject.meshCarps - Metabolismen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGrowth Hormone - Biosynthesis - Genetics - Secretionen_US
dc.subject.meshGrowth Hormone-Releasing Hormone - Physiologyen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIntracellular Membranes - Metabolismen_US
dc.subject.meshOsmolar Concentrationen_US
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptide - Pharmacology - Physiologyen_US
dc.subject.meshPituitary Gland - Cytology - Secretionen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshSignal Transduction - Physiologyen_US
dc.subject.meshVasoactive Intestinal Peptide - Secretionen_US
dc.titlePituitary Adenylate Cyclase-Activating Polypeptide (PACAP) as a Growth Hormone (GH)-releasing factor in grass carp. I. Functional coupling of cyclic adenosine 3′,5′-monophosphate and ca 2+/calmodulin- dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cellsen_US
dc.typeArticleen_US
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AOL=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/en.2005-0294en_US
dc.identifier.pmid16123157-
dc.identifier.scopuseid_2-s2.0-27844535742en_US
dc.identifier.hkuros113619-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27844535742&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume146en_US
dc.identifier.issue12en_US
dc.identifier.spage5407en_US
dc.identifier.epage5424en_US
dc.identifier.isiWOS:000233301000044-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, AOL=7403147570en_US
dc.identifier.scopusauthoridLi, W=36062301700en_US
dc.identifier.scopusauthoridLeung, CY=15731122000en_US
dc.identifier.scopusauthoridHuo, L=9275343500en_US
dc.identifier.scopusauthoridZhou, H=7404742310en_US

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