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Article: Determination of proanthocyanidins in fresh grapes and grape products using liquid chromatography with mass spectrometric detection

TitleDetermination of proanthocyanidins in fresh grapes and grape products using liquid chromatography with mass spectrometric detection
Authors
Issue Date2005
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0951-4198/
Citation
Rapid Communications In Mass Spectrometry, 2005, v. 19 n. 14, p. 2062-2068 How to Cite?
AbstractFresh grapes and grape products, such as grape wine and grape juice, were analyzed for proanthocyanidins (PACs) using liquid chromatography with electrospray ionization mass spectrometric (MS) detection. PACs were successfully separated and analyzed on the basis of their protonated molecules, allowing the identification of PACs in different degrees of polymerization from monomers to oligomers (up to 7 units), and in various isomeric forms. Using reversed-phase high-performance liquid chromatography (HPLC) combined with MS detection, the PAC monomers, (+)-catechin (C), (-)-epicatechin (EC), (-)-catechin gallate (CG), and (-)-epicatechin gallate (ECG), were successfully quantified using selected ion monitoring (SIM) mode. Standard curves were fitted for each PAC ranging from 43.8 to 5600ng/mL for C, from 42.2 to 5400 ng/mL for EC, from 36.7 to 4700 ng/mL for CG, and from 39.8 to 5100 ng/mL for ECG. Good linearity (r2 > 0.999) was achieved for each analyte. The accuracy and precision (RSD) were within 10% (n = 8) at the limit of detection. This method allows direct quantification of monomeric PACs in fresh grapes and grape-derived products. Additionally, flow injection analysis (FIA) was applied to estimate the concentration levels of PAC oligomers by comparing their FIA-MS peak areas, which were well correlated (r2 = 0.936) to the total concentrations of PAC monomers. Copyright © 2005 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/178895
ISSN
2015 Impact Factor: 2.226
2015 SCImago Journal Rankings: 0.819
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, Qen_US
dc.contributor.authorWang, Men_US
dc.contributor.authorSimon, JEen_US
dc.date.accessioned2012-12-19T09:50:31Z-
dc.date.available2012-12-19T09:50:31Z-
dc.date.issued2005en_US
dc.identifier.citationRapid Communications In Mass Spectrometry, 2005, v. 19 n. 14, p. 2062-2068en_US
dc.identifier.issn0951-4198en_US
dc.identifier.urihttp://hdl.handle.net/10722/178895-
dc.description.abstractFresh grapes and grape products, such as grape wine and grape juice, were analyzed for proanthocyanidins (PACs) using liquid chromatography with electrospray ionization mass spectrometric (MS) detection. PACs were successfully separated and analyzed on the basis of their protonated molecules, allowing the identification of PACs in different degrees of polymerization from monomers to oligomers (up to 7 units), and in various isomeric forms. Using reversed-phase high-performance liquid chromatography (HPLC) combined with MS detection, the PAC monomers, (+)-catechin (C), (-)-epicatechin (EC), (-)-catechin gallate (CG), and (-)-epicatechin gallate (ECG), were successfully quantified using selected ion monitoring (SIM) mode. Standard curves were fitted for each PAC ranging from 43.8 to 5600ng/mL for C, from 42.2 to 5400 ng/mL for EC, from 36.7 to 4700 ng/mL for CG, and from 39.8 to 5100 ng/mL for ECG. Good linearity (r2 > 0.999) was achieved for each analyte. The accuracy and precision (RSD) were within 10% (n = 8) at the limit of detection. This method allows direct quantification of monomeric PACs in fresh grapes and grape-derived products. Additionally, flow injection analysis (FIA) was applied to estimate the concentration levels of PAC oligomers by comparing their FIA-MS peak areas, which were well correlated (r2 = 0.936) to the total concentrations of PAC monomers. Copyright © 2005 John Wiley & Sons, Ltd.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0951-4198/en_US
dc.relation.ispartofRapid Communications in Mass Spectrometryen_US
dc.subject.meshChromatography, Liquid - Methodsen_US
dc.subject.meshFood Analysis - Instrumentationen_US
dc.subject.meshMass Spectrometry - Methodsen_US
dc.subject.meshProanthocyanidins - Analysisen_US
dc.subject.meshVitis - Chemistryen_US
dc.titleDetermination of proanthocyanidins in fresh grapes and grape products using liquid chromatography with mass spectrometric detectionen_US
dc.typeArticleen_US
dc.identifier.emailWang, M: mfwang@hku.hken_US
dc.identifier.authorityWang, M=rp00800en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/rcm.2029en_US
dc.identifier.pmid15988717-
dc.identifier.scopuseid_2-s2.0-22744448770en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-22744448770&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume19en_US
dc.identifier.issue14en_US
dc.identifier.spage2062en_US
dc.identifier.epage2068en_US
dc.identifier.isiWOS:000230666700021-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWu, Q=7404602467en_US
dc.identifier.scopusauthoridWang, M=7406691844en_US
dc.identifier.scopusauthoridSimon, JE=7403956069en_US

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