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Article: Endosperm protein accumulation in wild and cultivated barley and their cross grown in spike culture

TitleEndosperm protein accumulation in wild and cultivated barley and their cross grown in spike culture
Authors
KeywordsBarley
Hordein
Hordeum Spontaneum
Hordeum Vulgare
Protein Selection
Salt Soluble Protein
Spike Culture
Wild Barley
Issue Date1990
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0014-2336
Citation
Euphytica, 1990, v. 48 n. 3, p. 225-231 How to Cite?
AbstractThe high protein wild relatives of cultivated cereals have proven difficult to utilize in plant breeding by direct selection for high grain protein percentage, and hence alternative selection criteria are needed. In this study, a spike culture method was used to measure differences in protein accumulation between wild and cultivated barley, and their cross, at different levels of nitrogen supply. Three genotypes, barley cultivar Hordeum vulgare L. cv. Clipper, a wild barley accession H. spontaneum Koch line 363, and a high protein F5 line (38.4) derived from their cross, were grown from 8 to 27 days after flowering in in vitro spike culture. Nitrogen supply in the culture medium was either 0.4 g/l or 2.0 g/l of N supplied as NH4NO3. Spikes were harvested at approximately 3 day intervals during grain development, and salt soluble and hordein protein fractions were measured. Lines 363 and 38.4 differed from 'Clipper' in having extremely high initial rates of protein accumulation, even at 0.4 g/l N. In high nitrogen conditions all three genotypes reached similar salt soluble plus hordein protein levels. Hordein-1 and hordein-2 fractions were measured separately; the percent of hordein-1 was higher in lines 363 and 38.4 than in 'Clipper' at 0.4 g/l N. For all parameters measured, pot-grown spikes of matching age were harvested and were shown to be similar to the 0.4 g/l N treatment. The possible utilization of spike culture for identification of critical protein accumulation parameters is discussed, in relation to their possible utilization in breeding. © 1990 Kluwer Academic Publishers.
Persistent Identifierhttp://hdl.handle.net/10722/178877
ISSN
2015 Impact Factor: 1.618
2015 SCImago Journal Rankings: 0.690
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCorke, Hen_US
dc.contributor.authorAtsmon, Den_US
dc.date.accessioned2012-12-19T09:50:20Z-
dc.date.available2012-12-19T09:50:20Z-
dc.date.issued1990en_US
dc.identifier.citationEuphytica, 1990, v. 48 n. 3, p. 225-231en_US
dc.identifier.issn0014-2336en_US
dc.identifier.urihttp://hdl.handle.net/10722/178877-
dc.description.abstractThe high protein wild relatives of cultivated cereals have proven difficult to utilize in plant breeding by direct selection for high grain protein percentage, and hence alternative selection criteria are needed. In this study, a spike culture method was used to measure differences in protein accumulation between wild and cultivated barley, and their cross, at different levels of nitrogen supply. Three genotypes, barley cultivar Hordeum vulgare L. cv. Clipper, a wild barley accession H. spontaneum Koch line 363, and a high protein F5 line (38.4) derived from their cross, were grown from 8 to 27 days after flowering in in vitro spike culture. Nitrogen supply in the culture medium was either 0.4 g/l or 2.0 g/l of N supplied as NH4NO3. Spikes were harvested at approximately 3 day intervals during grain development, and salt soluble and hordein protein fractions were measured. Lines 363 and 38.4 differed from 'Clipper' in having extremely high initial rates of protein accumulation, even at 0.4 g/l N. In high nitrogen conditions all three genotypes reached similar salt soluble plus hordein protein levels. Hordein-1 and hordein-2 fractions were measured separately; the percent of hordein-1 was higher in lines 363 and 38.4 than in 'Clipper' at 0.4 g/l N. For all parameters measured, pot-grown spikes of matching age were harvested and were shown to be similar to the 0.4 g/l N treatment. The possible utilization of spike culture for identification of critical protein accumulation parameters is discussed, in relation to their possible utilization in breeding. © 1990 Kluwer Academic Publishers.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0014-2336en_US
dc.relation.ispartofEuphyticaen_US
dc.subjectBarleyen_US
dc.subjectHordeinen_US
dc.subjectHordeum Spontaneumen_US
dc.subjectHordeum Vulgareen_US
dc.subjectProtein Selectionen_US
dc.subjectSalt Soluble Proteinen_US
dc.subjectSpike Cultureen_US
dc.subjectWild Barleyen_US
dc.titleEndosperm protein accumulation in wild and cultivated barley and their cross grown in spike cultureen_US
dc.typeArticleen_US
dc.identifier.emailCorke, H: harold@hku.hken_US
dc.identifier.authorityCorke, H=rp00688en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00023654en_US
dc.identifier.scopuseid_2-s2.0-1842358550en_US
dc.identifier.volume48en_US
dc.identifier.issue3en_US
dc.identifier.spage225en_US
dc.identifier.epage231en_US
dc.identifier.isiWOS:A1990DQ35900005-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridCorke, H=7007102942en_US
dc.identifier.scopusauthoridAtsmon, D=24532939800en_US

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