File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: An agglutinating chitinase with two chitin-binding domains confers fungal protection in transgenic potato

TitleAn agglutinating chitinase with two chitin-binding domains confers fungal protection in transgenic potato
Authors
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2005, v. 220 n. 5, p. 717-730 How to Cite?
AbstractBrassica juncea BjCHI1 is a unique chitinase with two chitin-binding domains. Here, we show that, unlike other chitinases, potato-expressed BjCHI1 shows hemagglutination ability. BjCHI1 expression in B. juncea seedlings is induced by Rhizoctonia solani infection, suggesting its protective role against this fungus. To verify this, transgenic potato (Solanum tuberosum L. cv. Desiree) plants expressing BjCHI1 generated by Agrobacterium-mediated transformation were challenged with R. solani. We also transformed potato with a cDNA encoding Hevea brasiliensis β-1,3-glucanase, designated HbGLU, and a pBI121-derivative that contains cDNAs encoding both BjCHI1 and HbGLU. In vitro fungal bioassays using Trichoderma viride showed that extracts from transgenic potato lines co-expressing BjCHI1 and HbGLU inhibited fungal growth better than extracts from transgenic potato expressing either BjCHI1 or HbGLU, suggesting a synergistic effect. Consistently, in vivo fungal bioassays with soil-borne R. solani on young transgenic potato plants indicated that the co-expressing plants showed healthier root development than untransformed plants or those that expressed either BjCHI1 or HbGLU. Light microscopy and transmission electron microscopy revealed abundant intact R. solani hyphae and monilioid cells in untransformed roots and disintegrated fungus in the BjCHI1-expressing and the BjCHI1 and HbGLU co-expressing plants. Observations of collapsed epidermal cells in the co-expressing potato roots suggest that these proteins effectively degrade the fungal cell wall, producing elicitors that initiate other defense responses causing epidermal cell collapse that ultimately restricts further fungal penetration. © Springer-Verlag 2004.
Persistent Identifierhttp://hdl.handle.net/10722/178866
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChye, MLen_US
dc.contributor.authorZhao, KJen_US
dc.contributor.authorHe, ZMen_US
dc.contributor.authorRamalingam, Sen_US
dc.contributor.authorFung, KLen_US
dc.date.accessioned2012-12-19T09:50:16Z-
dc.date.available2012-12-19T09:50:16Z-
dc.date.issued2005en_US
dc.identifier.citationPlanta, 2005, v. 220 n. 5, p. 717-730en_US
dc.identifier.issn0032-0935en_US
dc.identifier.urihttp://hdl.handle.net/10722/178866-
dc.description.abstractBrassica juncea BjCHI1 is a unique chitinase with two chitin-binding domains. Here, we show that, unlike other chitinases, potato-expressed BjCHI1 shows hemagglutination ability. BjCHI1 expression in B. juncea seedlings is induced by Rhizoctonia solani infection, suggesting its protective role against this fungus. To verify this, transgenic potato (Solanum tuberosum L. cv. Desiree) plants expressing BjCHI1 generated by Agrobacterium-mediated transformation were challenged with R. solani. We also transformed potato with a cDNA encoding Hevea brasiliensis β-1,3-glucanase, designated HbGLU, and a pBI121-derivative that contains cDNAs encoding both BjCHI1 and HbGLU. In vitro fungal bioassays using Trichoderma viride showed that extracts from transgenic potato lines co-expressing BjCHI1 and HbGLU inhibited fungal growth better than extracts from transgenic potato expressing either BjCHI1 or HbGLU, suggesting a synergistic effect. Consistently, in vivo fungal bioassays with soil-borne R. solani on young transgenic potato plants indicated that the co-expressing plants showed healthier root development than untransformed plants or those that expressed either BjCHI1 or HbGLU. Light microscopy and transmission electron microscopy revealed abundant intact R. solani hyphae and monilioid cells in untransformed roots and disintegrated fungus in the BjCHI1-expressing and the BjCHI1 and HbGLU co-expressing plants. Observations of collapsed epidermal cells in the co-expressing potato roots suggest that these proteins effectively degrade the fungal cell wall, producing elicitors that initiate other defense responses causing epidermal cell collapse that ultimately restricts further fungal penetration. © Springer-Verlag 2004.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_US
dc.relation.ispartofPlantaen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshChitinase - Genetics - Metabolism - Physiologyen_US
dc.subject.meshGene Expression Regulation, Planten_US
dc.subject.meshGlucan 1,3-Beta-Glucosidase - Genetics - Metabolismen_US
dc.subject.meshHevea - Enzymologyen_US
dc.subject.meshMustard Plant - Enzymologyen_US
dc.subject.meshPlant Diseases - Genetics - Microbiologyen_US
dc.subject.meshPlant Roots - Microbiology - Ultrastructureen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRhizoctoniaen_US
dc.subject.meshSolanum Tuberosum - Geneticsen_US
dc.titleAn agglutinating chitinase with two chitin-binding domains confers fungal protection in transgenic potatoen_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00425-004-1391-6en_US
dc.identifier.pmid15490228-
dc.identifier.scopuseid_2-s2.0-15444365716en_US
dc.identifier.hkuros100173-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15444365716&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume220en_US
dc.identifier.issue5en_US
dc.identifier.spage717en_US
dc.identifier.epage730en_US
dc.identifier.isiWOS:000227441900008-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US
dc.identifier.scopusauthoridZhao, KJ=7202071954en_US
dc.identifier.scopusauthoridHe, ZM=15059027100en_US
dc.identifier.scopusauthoridRamalingam, S=8709830400en_US
dc.identifier.scopusauthoridFung, KL=7202935039en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats