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Article: Kinetics of adsorption and desorption of aflatoxin B1 by viable and nonviable bacteria

TitleKinetics of adsorption and desorption of aflatoxin B1 by viable and nonviable bacteria
Authors
Issue Date2003
Citation
Journal Of Food Protection, 2003, v. 66 n. 3, p. 426-430 How to Cite?
AbstractThe reactions involved in the binding (adsorption) and release (desorption) of aflatoxin B1 (AFB1) to and from the surface of bacteria were investigated. Viable and heat-killed Lactobacillus rhamnosus GG, L. rhamnosus LC-705, and Propionibacterium freudenreichii subsp. shermanii JS were incubated in phosphate-buffered saline containing variable concentrations (0.0017 to 13.3 μg/ml) of AFB1. The relationship between the bacterial surface hydrophobicity and the AFB1 adsorption affinity was also investigated. A linear relationship was observed between the specific rate of AFB1 adsorption and the AFB1 concentration for all bacteria. The nature of desorption of adsorbed AFB1 was investigated by repetitive aqueous washes. A linear relationship was observed between the natural log value of the concentration of AFB1 adsorbed and the number of washes for all bacteria studied. The desorption constants were strain-dependent and were lower for heat-killed bacteria than for viable bacteria. Heat treatment appears to alter the surface properties of the bacteria rather than expose new adsorption sites. No correlation was found between the hydrophobicity and the AFB1 adsorption affinity.
Persistent Identifierhttp://hdl.handle.net/10722/178831
ISSN
2015 Impact Factor: 1.609
2015 SCImago Journal Rankings: 0.966
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, YKen_US
dc.contributor.authorElNezami, Hen_US
dc.contributor.authorHaskard, CAen_US
dc.contributor.authorGratz, Sen_US
dc.contributor.authorPuong, KYen_US
dc.contributor.authorSalminen, Sen_US
dc.contributor.authorMykkänen, Hen_US
dc.date.accessioned2012-12-19T09:50:00Z-
dc.date.available2012-12-19T09:50:00Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Food Protection, 2003, v. 66 n. 3, p. 426-430en_US
dc.identifier.issn0362-028Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/178831-
dc.description.abstractThe reactions involved in the binding (adsorption) and release (desorption) of aflatoxin B1 (AFB1) to and from the surface of bacteria were investigated. Viable and heat-killed Lactobacillus rhamnosus GG, L. rhamnosus LC-705, and Propionibacterium freudenreichii subsp. shermanii JS were incubated in phosphate-buffered saline containing variable concentrations (0.0017 to 13.3 μg/ml) of AFB1. The relationship between the bacterial surface hydrophobicity and the AFB1 adsorption affinity was also investigated. A linear relationship was observed between the specific rate of AFB1 adsorption and the AFB1 concentration for all bacteria. The nature of desorption of adsorbed AFB1 was investigated by repetitive aqueous washes. A linear relationship was observed between the natural log value of the concentration of AFB1 adsorbed and the number of washes for all bacteria studied. The desorption constants were strain-dependent and were lower for heat-killed bacteria than for viable bacteria. Heat treatment appears to alter the surface properties of the bacteria rather than expose new adsorption sites. No correlation was found between the hydrophobicity and the AFB1 adsorption affinity.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Food Protectionen_US
dc.subject.meshAdsorptionen_US
dc.subject.meshAflatoxin B1 - Metabolismen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshFood Handling - Methodsen_US
dc.subject.meshFood Microbiologyen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshKineticsen_US
dc.subject.meshLactobacillus - Metabolismen_US
dc.subject.meshPropionibacterium - Metabolismen_US
dc.subject.meshWater - Metabolismen_US
dc.titleKinetics of adsorption and desorption of aflatoxin B1 by viable and nonviable bacteriaen_US
dc.typeArticleen_US
dc.identifier.emailElNezami, H: elnezami@hkucc.hku.hken_US
dc.identifier.authorityElNezami, H=rp00694en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12636295-
dc.identifier.scopuseid_2-s2.0-0344406136en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344406136&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume66en_US
dc.identifier.issue3en_US
dc.identifier.spage426en_US
dc.identifier.epage430en_US
dc.identifier.isiWOS:000181602100011-
dc.identifier.scopusauthoridLee, YK=8135150600en_US
dc.identifier.scopusauthoridElNezami, H=6603690577en_US
dc.identifier.scopusauthoridHaskard, CA=6602356857en_US
dc.identifier.scopusauthoridGratz, S=9242677500en_US
dc.identifier.scopusauthoridPuong, KY=8896662000en_US
dc.identifier.scopusauthoridSalminen, S=7102912002en_US
dc.identifier.scopusauthoridMykkänen, H=7003915985en_US

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