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Article: High cell density culture of Chlamydomonas reinhardtii on acetate using fed-batch and hollow-fibre cell-recycle systems

TitleHigh cell density culture of Chlamydomonas reinhardtii on acetate using fed-batch and hollow-fibre cell-recycle systems
Authors
KeywordsAcetate
Chlamydomonas Reinhardtii
Fed Batch
High Cell Density
Hollow-Fibre Cell-Recycle System
Issue Date1996
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortech
Citation
Bioresource Technology, 1996, v. 55 n. 2, p. 103-110 How to Cite?
AbstractFed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0.2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0.1/h and a feed acetate concentration of 1.7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0.083 g/l/h was achieved at a bleed ratio of 0.5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates. | Fed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures, it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0·2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0·1/h and a feed acetate concentration of 1·7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0·083 g/l/h was achieved at a bleed ratio of 0·5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates.
Persistent Identifierhttp://hdl.handle.net/10722/178826
ISSN
2015 Impact Factor: 4.917
2015 SCImago Journal Rankings: 2.303
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Fen_US
dc.contributor.authorJohns, MRen_US
dc.date.accessioned2012-12-19T09:49:58Z-
dc.date.available2012-12-19T09:49:58Z-
dc.date.issued1996en_US
dc.identifier.citationBioresource Technology, 1996, v. 55 n. 2, p. 103-110en_US
dc.identifier.issn0960-8524en_US
dc.identifier.urihttp://hdl.handle.net/10722/178826-
dc.description.abstractFed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0.2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0.1/h and a feed acetate concentration of 1.7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0.083 g/l/h was achieved at a bleed ratio of 0.5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates. | Fed-batch culture was performed to minimise growth inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture. Although the cell concentration obtained was higher than in batch cultures, it was eventually constrained by high sodium concentrations, which accumulated in the fermenter. The use of a continuous-flow, hollow-fibre cell-recycle system (HFCRS) using a membrane with a 0·2 μm pore size was successfully employed to overcome this problem. The HFCRS was run with no cell bleed to obtain a cell concentration of 9 g/l at a dilution rate of 0·1/h and a feed acetate concentration of 1·7 g/l. However, the cell growth yield under these conditions was poor. Using the same dilution rate and feed acetate concentration, a superior cell productivity of 0·083 g/l/h was achieved at a bleed ratio of 0·5. An unstructured mathematical model, incorporating the maintenance coefficient, was derived for the HFCRS. The model gave good agreement with experimental results, particularly at high bleed ratios, and predicted that higher cell concentration and productivity could be obtained using the HFCRS with a higher feed acetate concentration and dilution rate. The results demonstrate that the HFCRS is well suited to obtaining high concentrations of microalgae grown heterotrophically on inhibitory substrates.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biortechen_US
dc.relation.ispartofBioresource Technologyen_US
dc.subjectAcetateen_US
dc.subjectChlamydomonas Reinhardtiien_US
dc.subjectFed Batchen_US
dc.subjectHigh Cell Densityen_US
dc.subjectHollow-Fibre Cell-Recycle Systemen_US
dc.titleHigh cell density culture of Chlamydomonas reinhardtii on acetate using fed-batch and hollow-fibre cell-recycle systemsen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0960-8524(95)00141-7en_US
dc.identifier.scopuseid_2-s2.0-0343773296en_US
dc.identifier.hkuros12305-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0343773296&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume55en_US
dc.identifier.issue2en_US
dc.identifier.spage103en_US
dc.identifier.epage110en_US
dc.identifier.isiWOS:A1996UP45300002-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridJohns, MR=7202660598en_US

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