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Article: High cell density culture of the diatom Nitzschia laevis for eicosapentaenoic acid production: Fed-batch development

TitleHigh cell density culture of the diatom Nitzschia laevis for eicosapentaenoic acid production: Fed-batch development
Authors
KeywordsC:N Ratio
Diatom
Eicosapentaenoic Acid
Fed-Batch
High Cell Density
Nitzschia Laevis
Issue Date2002
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 2002, v. 37 n. 12, p. 1447-1453 How to Cite?
AbstractA fed-batch process was developed for high cell density culture of the diatom Nitzschia laevis for enhanced production of eicosapentaenoic acid (EPA). Firstly, among the various medium components, glucose (Glu) was identified as the limiting substrate while nitrate (NO3 -), tryptone (Tr) and yeast extract (Ye) were found to promote cell growth by enhancing specific growth rate. Therefore, these components were considered essential and were included in the feed medium for subsequent fed-batch cultivation. With the optimized ratio of NO3 - -:Tr:Ye being 1:2.6:1.3 (by weight), the relative proportions of glucose to the nitrogen sources in the feed were investigated. The optimal ratios of Glu: NO3 - for specific growth rate and EPA productivity were both determined to be 32:1 (by weight). Finally, based on the residual glucose concentration in the culture, a continuous medium feeding strategy for fed-batch fermenter cultivation was developed, with which, the maximal cell dry weight and EPA yield obtained were 22.1 g l-1 and 695 mg l-1, respectively, which were great improvements over those of batch cultures. © 2002 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/178773
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWen, ZYen_US
dc.contributor.authorJiang, Yen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:49:40Z-
dc.date.available2012-12-19T09:49:40Z-
dc.date.issued2002en_US
dc.identifier.citationProcess Biochemistry, 2002, v. 37 n. 12, p. 1447-1453en_US
dc.identifier.issn1359-5113en_US
dc.identifier.urihttp://hdl.handle.net/10722/178773-
dc.description.abstractA fed-batch process was developed for high cell density culture of the diatom Nitzschia laevis for enhanced production of eicosapentaenoic acid (EPA). Firstly, among the various medium components, glucose (Glu) was identified as the limiting substrate while nitrate (NO3 -), tryptone (Tr) and yeast extract (Ye) were found to promote cell growth by enhancing specific growth rate. Therefore, these components were considered essential and were included in the feed medium for subsequent fed-batch cultivation. With the optimized ratio of NO3 - -:Tr:Ye being 1:2.6:1.3 (by weight), the relative proportions of glucose to the nitrogen sources in the feed were investigated. The optimal ratios of Glu: NO3 - for specific growth rate and EPA productivity were both determined to be 32:1 (by weight). Finally, based on the residual glucose concentration in the culture, a continuous medium feeding strategy for fed-batch fermenter cultivation was developed, with which, the maximal cell dry weight and EPA yield obtained were 22.1 g l-1 and 695 mg l-1, respectively, which were great improvements over those of batch cultures. © 2002 Elsevier Science Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_US
dc.relation.ispartofProcess Biochemistryen_US
dc.rightsProcess Biochemistry. Copyright © Elsevier Ltd.-
dc.subjectC:N Ratioen_US
dc.subjectDiatomen_US
dc.subjectEicosapentaenoic Aciden_US
dc.subjectFed-Batchen_US
dc.subjectHigh Cell Densityen_US
dc.subjectNitzschia Laevisen_US
dc.titleHigh cell density culture of the diatom Nitzschia laevis for eicosapentaenoic acid production: Fed-batch developmenten_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0032-9592(02)00034-1en_US
dc.identifier.scopuseid_2-s2.0-0036661106en_US
dc.identifier.hkuros71929-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036661106&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume37en_US
dc.identifier.issue12en_US
dc.identifier.spage1447en_US
dc.identifier.epage1453en_US
dc.identifier.isiWOS:000176219300014-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWen, ZY=14012373200en_US
dc.identifier.scopusauthoridJiang, Y=24605346600en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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