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Article: Binding rather than metabolism may explain the interaction of two food-grade Lactobacillus strains with zearalenone and its derivative ά-zearalenol

TitleBinding rather than metabolism may explain the interaction of two food-grade Lactobacillus strains with zearalenone and its derivative ά-zearalenol
Authors
Issue Date2002
Citation
Applied And Environmental Microbiology, 2002, v. 68 n. 7, p. 3545-3549 How to Cite?
AbstractThe interaction between two Fusarium mycotoxins, zearalenone (ZEN) and its derivative ά-zearalenol (α-ZOL), with two food-grade strains of Lactobacillus was investigated. The mycotoxins (2 μg ml-1) were incubated with either Lactobacillus rhamnosus strain GG or L. rhamnosus strain LC705. A considerable proportion (38 to 46%) of both toxins was recovered from the bacterial pellet, and no degradation products of ZEN and ά-ZOL were detected in the high-performance liquid chromatograms of the supernatant of the culturing media and the methanol extract of the pellet. Both heat-treated and acid-treated bacteria were capable of removing the toxins, indicating that binding, not metabolism, is the mechanism by which the toxins are removed from the media. Binding of ZEN or α4́-ZOL by lyophilized L. rhamnosus GG and L. rhamnosus LC705 was a rapid reaction: approximately 55% of the toxins were bound instantly after mixing with the bacteria. Binding was dependent on the bacterial concentration, and coincubation of ZEN with ά-ZOL significantly affected the percentage of the toxin bound, indicating that these toxins may share the same binding site on the bacterial surface. These results can be exploited in developing a new approach for detoxification of mycotoxins from foods and feeds.
Persistent Identifierhttp://hdl.handle.net/10722/178765
ISSN
2015 Impact Factor: 3.823
2015 SCImago Journal Rankings: 1.891
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorElNezami, Hen_US
dc.contributor.authorPolychronaki, Nen_US
dc.contributor.authorSalminen, Sen_US
dc.contributor.authorMykkänen, Hen_US
dc.date.accessioned2012-12-19T09:49:37Z-
dc.date.available2012-12-19T09:49:37Z-
dc.date.issued2002en_US
dc.identifier.citationApplied And Environmental Microbiology, 2002, v. 68 n. 7, p. 3545-3549en_US
dc.identifier.issn0099-2240en_US
dc.identifier.urihttp://hdl.handle.net/10722/178765-
dc.description.abstractThe interaction between two Fusarium mycotoxins, zearalenone (ZEN) and its derivative ά-zearalenol (α-ZOL), with two food-grade strains of Lactobacillus was investigated. The mycotoxins (2 μg ml-1) were incubated with either Lactobacillus rhamnosus strain GG or L. rhamnosus strain LC705. A considerable proportion (38 to 46%) of both toxins was recovered from the bacterial pellet, and no degradation products of ZEN and ά-ZOL were detected in the high-performance liquid chromatograms of the supernatant of the culturing media and the methanol extract of the pellet. Both heat-treated and acid-treated bacteria were capable of removing the toxins, indicating that binding, not metabolism, is the mechanism by which the toxins are removed from the media. Binding of ZEN or α4́-ZOL by lyophilized L. rhamnosus GG and L. rhamnosus LC705 was a rapid reaction: approximately 55% of the toxins were bound instantly after mixing with the bacteria. Binding was dependent on the bacterial concentration, and coincubation of ZEN with ά-ZOL significantly affected the percentage of the toxin bound, indicating that these toxins may share the same binding site on the bacterial surface. These results can be exploited in developing a new approach for detoxification of mycotoxins from foods and feeds.en_US
dc.languageengen_US
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.subject.meshAflatoxins - Metabolismen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshEstrogens, Non-Steroidal - Metabolismen_US
dc.subject.meshLactobacillus - Metabolismen_US
dc.subject.meshTrichothecenes - Metabolismen_US
dc.subject.meshZearalenone - Metabolismen_US
dc.subject.meshZeranol - Analogs & Derivatives - Metabolismen_US
dc.titleBinding rather than metabolism may explain the interaction of two food-grade Lactobacillus strains with zearalenone and its derivative ά-zearalenolen_US
dc.typeArticleen_US
dc.identifier.emailElNezami, H: elnezami@hkucc.hku.hken_US
dc.identifier.authorityElNezami, H=rp00694en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/AEM.68.7.3545-3549.2002en_US
dc.identifier.pmid12089040en_US
dc.identifier.scopuseid_2-s2.0-0036306526en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036306526&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume68en_US
dc.identifier.issue7en_US
dc.identifier.spage3545en_US
dc.identifier.epage3549en_US
dc.identifier.isiWOS:000176631600048-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridElNezami, H=6603690577en_US
dc.identifier.scopusauthoridPolychronaki, N=6505944468en_US
dc.identifier.scopusauthoridSalminen, S=7102912002en_US
dc.identifier.scopusauthoridMykkänen, H=7003915985en_US

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