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Article: Immunoregulatory and antioxidant performance of α-tocopherol and selenium on human lymphocytes

TitleImmunoregulatory and antioxidant performance of α-tocopherol and selenium on human lymphocytes
Authors
Issue Date2002
Citation
Biological Trace Element Research, 2002, v. 86 n. 2, p. 123-136 How to Cite?
AbstractThe role of α-tocopherol (α-toco) and selenium (Se) on human lymphocyte oxidative stress and T-cells proliferation were studied by flow cytometry. We measured the hydrogen peroxide and glutathione levels in cultured human T-lymphocytes and the proliferation of their subsets: T-helper/inducer, T-suppressor/cytotoxic, and natural killer and interleukin-2 receptors upon stimulation by the mitogens phytohemaglutinin (PHA) and lipopolysaccharide (LPS). The results indicate that early stimulation by mitogens is affected by the glutathione and hydrogen peroxide status of the T-lymphocytes. The addition of 100 μM or 500 μM α-toco or 0.5 μM Se alone shows weak antioxidant and immunostimulant properties. When combined, an enhanced antioxidant and immunoregulatory effect was observed. The present findings indicate that α-toco and Se have interactive effects as oxygen radical scavengers, thus promoting human lymphocyte response to antigens. This suggests that micronutrient status is an important factor in considering when interpreting the results of in vitro assays of lymphocyte function.
Persistent Identifierhttp://hdl.handle.net/10722/178764
ISSN
2015 Impact Factor: 1.798
2015 SCImago Journal Rankings: 0.652
References

 

DC FieldValueLanguage
dc.contributor.authorLee, CYJen_US
dc.contributor.authorWan, JMFen_US
dc.date.accessioned2012-12-19T09:49:36Z-
dc.date.available2012-12-19T09:49:36Z-
dc.date.issued2002en_US
dc.identifier.citationBiological Trace Element Research, 2002, v. 86 n. 2, p. 123-136en_US
dc.identifier.issn0163-4984en_US
dc.identifier.urihttp://hdl.handle.net/10722/178764-
dc.description.abstractThe role of α-tocopherol (α-toco) and selenium (Se) on human lymphocyte oxidative stress and T-cells proliferation were studied by flow cytometry. We measured the hydrogen peroxide and glutathione levels in cultured human T-lymphocytes and the proliferation of their subsets: T-helper/inducer, T-suppressor/cytotoxic, and natural killer and interleukin-2 receptors upon stimulation by the mitogens phytohemaglutinin (PHA) and lipopolysaccharide (LPS). The results indicate that early stimulation by mitogens is affected by the glutathione and hydrogen peroxide status of the T-lymphocytes. The addition of 100 μM or 500 μM α-toco or 0.5 μM Se alone shows weak antioxidant and immunostimulant properties. When combined, an enhanced antioxidant and immunoregulatory effect was observed. The present findings indicate that α-toco and Se have interactive effects as oxygen radical scavengers, thus promoting human lymphocyte response to antigens. This suggests that micronutrient status is an important factor in considering when interpreting the results of in vitro assays of lymphocyte function.en_US
dc.languageengen_US
dc.relation.ispartofBiological Trace Element Researchen_US
dc.subject.meshAntigens, Cd4 - Drug Effectsen_US
dc.subject.meshAntigens, Cd56 - Drug Effectsen_US
dc.subject.meshAntigens, Cd8 - Drug Effectsen_US
dc.subject.meshAntioxidants - Metabolism - Pharmacologyen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDrug Synergismen_US
dc.subject.meshGlutathione - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen Peroxide - Metabolismen_US
dc.subject.meshLipopolysaccharides - Toxicityen_US
dc.subject.meshLymphocyte Subsets - Drug Effectsen_US
dc.subject.meshLymphocytes - Drug Effects - Immunologyen_US
dc.subject.meshMitogens - Toxicityen_US
dc.subject.meshPhytohemagglutinins - Toxicityen_US
dc.subject.meshReceptors, Interleukin-2 - Drug Effectsen_US
dc.subject.meshSelenium - Pharmacologyen_US
dc.subject.meshAlpha-Tocopherol - Pharmacologyen_US
dc.titleImmunoregulatory and antioxidant performance of α-tocopherol and selenium on human lymphocytesen_US
dc.typeArticleen_US
dc.identifier.emailLee, CYJ: jettylee@hku.hken_US
dc.identifier.emailWan, JMF: jmfwan@hku.hken_US
dc.identifier.authorityLee, CYJ=rp01511en_US
dc.identifier.authorityWan, JMF=rp00798en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1385/BTER:86:2:123en_US
dc.identifier.pmid12008975-
dc.identifier.scopuseid_2-s2.0-0036240909en_US
dc.identifier.hkuros66208-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036240909&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume86en_US
dc.identifier.issue2en_US
dc.identifier.spage123en_US
dc.identifier.epage136en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, CYJ=13104265200en_US
dc.identifier.scopusauthoridWan, JMF=8930305000en_US

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