File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The in vivo and in vitro effects of chicken interferon α on infectious bursal disease virus and Newcastle disease virus infection

TitleThe in vivo and in vitro effects of chicken interferon α on infectious bursal disease virus and Newcastle disease virus infection
Authors
Issue Date2001
PublisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-html
Citation
Avian Diseases, 2001, v. 45 n. 2, p. 389-399 How to Cite?
AbstractThe in vitro and in vivo effects of chicken interferon α on infectious bursal disease virus (IBDV) infection were investigated in this study. A cDNA of interferon α was first cloned from a Chinese strain chicken Shiqi by reverse transcription-polymerase chain reaction. The deduced amino acid sequence has one amino acid substitution with chicken interferon α 1 at residue 65 (N to S) and two amino acid substitutions with chicken interferon α 2 at residues 50 (N to S) and 58 (P to L), respectively. A prokaryotic expression system was employed to produce a large quantity of recombinant protein. Recombinant interferon was purified in a one-step process, and an optimal refolding process was devised. About 51% recombinant protein from inclusion bodies was refolded, and the final yield of the recombinant interferon reached 24.66 mg/liter culture. The recombinant interferon suppressed IBDV plaque formation in a dose-dependent manner and ameliorated IBDV and Newcastle disease virus infection in both specific-pathogen-free (SPF) and commercial chickens. The antiviral effect of interferon α is more significant in commercial chickens than in SPF chickens, and the route of administration affects the efficacy of interferon therapy. This is the first reported study of the effects of interferon α on IBDV infection.
Persistent Identifierhttp://hdl.handle.net/10722/178735
ISSN
2015 Impact Factor: 1.104
2015 SCImago Journal Rankings: 0.772
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMo, CWen_US
dc.contributor.authorCao, YCen_US
dc.contributor.authorLim, BLen_US
dc.date.accessioned2012-12-19T09:49:24Z-
dc.date.available2012-12-19T09:49:24Z-
dc.date.issued2001en_US
dc.identifier.citationAvian Diseases, 2001, v. 45 n. 2, p. 389-399en_US
dc.identifier.issn0005-2086en_US
dc.identifier.urihttp://hdl.handle.net/10722/178735-
dc.description.abstractThe in vitro and in vivo effects of chicken interferon α on infectious bursal disease virus (IBDV) infection were investigated in this study. A cDNA of interferon α was first cloned from a Chinese strain chicken Shiqi by reverse transcription-polymerase chain reaction. The deduced amino acid sequence has one amino acid substitution with chicken interferon α 1 at residue 65 (N to S) and two amino acid substitutions with chicken interferon α 2 at residues 50 (N to S) and 58 (P to L), respectively. A prokaryotic expression system was employed to produce a large quantity of recombinant protein. Recombinant interferon was purified in a one-step process, and an optimal refolding process was devised. About 51% recombinant protein from inclusion bodies was refolded, and the final yield of the recombinant interferon reached 24.66 mg/liter culture. The recombinant interferon suppressed IBDV plaque formation in a dose-dependent manner and ameliorated IBDV and Newcastle disease virus infection in both specific-pathogen-free (SPF) and commercial chickens. The antiviral effect of interferon α is more significant in commercial chickens than in SPF chickens, and the route of administration affects the efficacy of interferon therapy. This is the first reported study of the effects of interferon α on IBDV infection.en_US
dc.languageengen_US
dc.publisherAmerican Association of Avian Pathologists, Inc. The Journal's web site is located at http://avdi.allenpress.com/avdionline/?request=index-htmlen_US
dc.relation.ispartofAvian Diseasesen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntiviral Agents - Chemistry - Immunology - Pharmacology - Therapeutic Useen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBirnaviridae Infections - Drug Therapy - Veterinaryen_US
dc.subject.meshChickensen_US
dc.subject.meshDna, Complementary - Analysisen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDrug Administration Routes - Veterinaryen_US
dc.subject.meshInfectious Bursal Disease Virus - Immunologyen_US
dc.subject.meshInterferon Type I - Chemistry - Genetics - Immunology - Therapeutic Useen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNewcastle Disease - Drug Therapyen_US
dc.subject.meshNewcastle Disease Virus - Immunologyen_US
dc.subject.meshPoultry Diseases - Drug Therapy - Virologyen_US
dc.subject.meshRecombinant Proteinsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Veterinaryen_US
dc.subject.meshSpecific Pathogen-Free Organismsen_US
dc.subject.meshTreatment Outcomeen_US
dc.titleThe in vivo and in vitro effects of chicken interferon α on infectious bursal disease virus and Newcastle disease virus infectionen_US
dc.typeArticleen_US
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_US
dc.identifier.authorityLim, BL=rp00744en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2307/1592978-
dc.identifier.pmid11417818-
dc.identifier.scopuseid_2-s2.0-0034976139en_US
dc.identifier.hkuros57451-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034976139&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue2en_US
dc.identifier.spage389en_US
dc.identifier.epage399en_US
dc.identifier.isiWOS:000169271700012-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMo, CW=7005256318en_US
dc.identifier.scopusauthoridCao, YC=25632098400en_US
dc.identifier.scopusauthoridLim, BL=7201983917en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats