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Article: Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection

TitleSimultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection
Authors
KeywordsColumn Liquid Chromatography
Pharmaceutical Preparations
Water- And Fat-Soluble Vitamins
Issue Date2001
PublisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://link.springer.com/journal/10337
Citation
Chromatographia, 2001, v. 54 n. 3-4, p. 270-273 How to Cite?
AbstractA novel method for the simultaneous determination of twelve water- and fat-soluble vitamins has been established by high-performance liquid chromatography with diode array detection. The vitamins were analyzed on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol- KH2PO4 buffer (0.1 M, pH 7.0)- water as mobile phase in a gradient. The linearity of calibration graphs was compound-dependent and the detection limits ranged from 0.02 μg mL-1 to 0.5 μg mL-1. The method was successfully applied to determine vitamins in pharmaceutical preparations. The recoveries were from 95.1% to 103% and the relative standard deviations were in the range of 0.9% to 4.5%.
Persistent Identifierhttp://hdl.handle.net/10722/178729
ISSN
2015 Impact Factor: 1.332
2015 SCImago Journal Rankings: 0.480
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:49:22Z-
dc.date.available2012-12-19T09:49:22Z-
dc.date.issued2001en_US
dc.identifier.citationChromatographia, 2001, v. 54 n. 3-4, p. 270-273en_US
dc.identifier.issn0009-5893en_US
dc.identifier.urihttp://hdl.handle.net/10722/178729-
dc.description.abstractA novel method for the simultaneous determination of twelve water- and fat-soluble vitamins has been established by high-performance liquid chromatography with diode array detection. The vitamins were analyzed on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol- KH2PO4 buffer (0.1 M, pH 7.0)- water as mobile phase in a gradient. The linearity of calibration graphs was compound-dependent and the detection limits ranged from 0.02 μg mL-1 to 0.5 μg mL-1. The method was successfully applied to determine vitamins in pharmaceutical preparations. The recoveries were from 95.1% to 103% and the relative standard deviations were in the range of 0.9% to 4.5%.en_US
dc.languageengen_US
dc.publisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://link.springer.com/journal/10337en_US
dc.relation.ispartofChromatographiaen_US
dc.subjectColumn Liquid Chromatographyen_US
dc.subjectPharmaceutical Preparationsen_US
dc.subjectWater- And Fat-Soluble Vitaminsen_US
dc.titleSimultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detectionen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF02492256-
dc.identifier.scopuseid_2-s2.0-0034868633en_US
dc.identifier.hkuros72650-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034868633&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume54en_US
dc.identifier.issue3-4en_US
dc.identifier.spage270en_US
dc.identifier.epage273en_US
dc.identifier.isiWOS:000170734900023-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLi, HB=25649944400en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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