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Article: A novel perfusion system for animal cell cultures by two step sequential sedimentation

TitleA novel perfusion system for animal cell cultures by two step sequential sedimentation
Authors
Issue Date2000
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotec
Citation
Journal Of Biotechnology, 2000, v. 79 n. 1, p. 1-11 How to Cite?
AbstractA novel perfusion system was developed for high density culture of animal cells. The system consists of an airlift bioreactor, a setting tank and a flat settler. Both the settling tank and flat settler have two connecting pipes for transporting the cells from and back to the reactor, respectively. Thus, the cell flow in the settlers can be controlled in uni-direction, avoiding the countercurrent flow of the cells. During perfusion cultures, the cells firstly settled in the settling tank, then, unsettled cells in the tank were transferred to the flat settler for re-settling. With the application of the system to hybridoma cell cultures, it was found that the maximum viable cell density, monoclonal antibody concentration and average productivity were 1.31x107 cells ml-1, 400 mg l-1 and 461 mg l-1 d-1, respectively, which were much higher than those of a batch culture. Both theoretical analysis and experimental results showed a much higher separation efficiency in such a two-step sedimentation system than that in a conventional one-step sedimentation system. In addition, the volumetric ratio of the sedimentation devices to the culture volume in our developed system is much lower, which may be potentially useful on an industrial-scale. Copyright (C) 2000 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/178723
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.064
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWen, ZYen_US
dc.contributor.authorTeng, XWen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T09:49:20Z-
dc.date.available2012-12-19T09:49:20Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Biotechnology, 2000, v. 79 n. 1, p. 1-11en_US
dc.identifier.issn0168-1656en_US
dc.identifier.urihttp://hdl.handle.net/10722/178723-
dc.description.abstractA novel perfusion system was developed for high density culture of animal cells. The system consists of an airlift bioreactor, a setting tank and a flat settler. Both the settling tank and flat settler have two connecting pipes for transporting the cells from and back to the reactor, respectively. Thus, the cell flow in the settlers can be controlled in uni-direction, avoiding the countercurrent flow of the cells. During perfusion cultures, the cells firstly settled in the settling tank, then, unsettled cells in the tank were transferred to the flat settler for re-settling. With the application of the system to hybridoma cell cultures, it was found that the maximum viable cell density, monoclonal antibody concentration and average productivity were 1.31x107 cells ml-1, 400 mg l-1 and 461 mg l-1 d-1, respectively, which were much higher than those of a batch culture. Both theoretical analysis and experimental results showed a much higher separation efficiency in such a two-step sedimentation system than that in a conventional one-step sedimentation system. In addition, the volumetric ratio of the sedimentation devices to the culture volume in our developed system is much lower, which may be potentially useful on an industrial-scale. Copyright (C) 2000 Elsevier Science B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotecen_US
dc.relation.ispartofJournal of Biotechnologyen_US
dc.rightsJournal of Biotechnology. Copyright © Elsevier BV.-
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Biosynthesisen_US
dc.subject.meshBioreactorsen_US
dc.subject.meshCell Counten_US
dc.subject.meshCell Culture Techniques - Instrumentationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Separationen_US
dc.subject.meshCell Survivalen_US
dc.subject.meshGravitationen_US
dc.subject.meshHybridomas - Cytology - Immunologyen_US
dc.subject.meshImmunoglobulin G - Biosynthesisen_US
dc.subject.meshImmunoglobulin Kappa-Chains - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshPerfusionen_US
dc.subject.meshRatsen_US
dc.titleA novel perfusion system for animal cell cultures by two step sequential sedimentationen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0168-1656(00)00219-4en_US
dc.identifier.pmid10817337-
dc.identifier.scopuseid_2-s2.0-0034647042en_US
dc.identifier.hkuros52886-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034647042&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume79en_US
dc.identifier.issue1en_US
dc.identifier.spage1en_US
dc.identifier.epage11en_US
dc.identifier.isiWOS:000086786600001-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridWen, ZY=14012373200en_US
dc.identifier.scopusauthoridTeng, XW=7005444936en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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