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Article: Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive

TitleExpression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive
Authors
Issue Date2000
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412
Citation
Plant Journal, 2000, v. 22 n. 5, p. 415-426 How to Cite?
Abstract3-Hydroxy-3-methylglutaryl-coenzymeA synthase (HMGS) is an enzyme in mevalonate biosynthesis. In plants, investigations have focused on HMG CoA reductase (HMGR) and less is known of the preceding enzyme, HMGS. To understand the regulation of HMGS, we have isolated a Brassica juncea cDNA encoding HMGS, BjHMGS1, for use as a hybridization probe in Northern blot analyses. BjHMGS is expressed in all plant organs and shows developmental regulation in flower, seed and seedling, with highest expression in early development. In seedlings, expression is highest in young hypocotyls and is induced during the greening of etiolated cotyledons. BjHMGS is down-regulated by abscisic acid, osmotic stress and dehydration, the effects of which arrested seedling growth. Thus BjHMGS expression shows correlation with rapid cell division and growth, like HMGR. This is not unexpected, as mevalonate is the precursor to many essential isoprenoid compounds, including sterols for membrane biogenesis. Wounding, methyl jasmonate or salicylic acid induce BjHMGS expression, suggesting that, like HMGR, HMGS is involved in defence. As in animals, coordinated regulation of HMGS with HMGR occurred in B. juncea upon germination and in response to salicylic acid. HMGS assays confirmed that Escherichia coli-expressed recombinant BjHMGS1 shows HMGS activity that is inhibited by F244, a specific inhibitor of HMGS. Southern blot analysis revealed gene families encoding HMGS in Brassica species and a summation of homologous genes in the fusion amphidiploid genome of B. juncea, a bi-parental species derived from diploids B. nigra and B. campestris.
Persistent Identifierhttp://hdl.handle.net/10722/178711
ISSN
2015 Impact Factor: 5.468
2015 SCImago Journal Rankings: 3.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAlex, Den_US
dc.contributor.authorBach, TJen_US
dc.contributor.authorChye, MLen_US
dc.date.accessioned2012-12-19T09:49:17Z-
dc.date.available2012-12-19T09:49:17Z-
dc.date.issued2000en_US
dc.identifier.citationPlant Journal, 2000, v. 22 n. 5, p. 415-426en_US
dc.identifier.issn0960-7412en_US
dc.identifier.urihttp://hdl.handle.net/10722/178711-
dc.description.abstract3-Hydroxy-3-methylglutaryl-coenzymeA synthase (HMGS) is an enzyme in mevalonate biosynthesis. In plants, investigations have focused on HMG CoA reductase (HMGR) and less is known of the preceding enzyme, HMGS. To understand the regulation of HMGS, we have isolated a Brassica juncea cDNA encoding HMGS, BjHMGS1, for use as a hybridization probe in Northern blot analyses. BjHMGS is expressed in all plant organs and shows developmental regulation in flower, seed and seedling, with highest expression in early development. In seedlings, expression is highest in young hypocotyls and is induced during the greening of etiolated cotyledons. BjHMGS is down-regulated by abscisic acid, osmotic stress and dehydration, the effects of which arrested seedling growth. Thus BjHMGS expression shows correlation with rapid cell division and growth, like HMGR. This is not unexpected, as mevalonate is the precursor to many essential isoprenoid compounds, including sterols for membrane biogenesis. Wounding, methyl jasmonate or salicylic acid induce BjHMGS expression, suggesting that, like HMGR, HMGS is involved in defence. As in animals, coordinated regulation of HMGS with HMGR occurred in B. juncea upon germination and in response to salicylic acid. HMGS assays confirmed that Escherichia coli-expressed recombinant BjHMGS1 shows HMGS activity that is inhibited by F244, a specific inhibitor of HMGS. Southern blot analysis revealed gene families encoding HMGS in Brassica species and a summation of homologous genes in the fusion amphidiploid genome of B. juncea, a bi-parental species derived from diploids B. nigra and B. campestris.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412en_US
dc.relation.ispartofPlant Journalen_US
dc.rightsThe Plant Journal. Copyright © Blackwell Publishing Ltd.-
dc.subject.meshAbscisic Acid - Pharmacologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBrassica - Enzymology - Genetics - Growth & Developmenten_US
dc.subject.meshDna Primersen_US
dc.subject.meshDna, Complementaryen_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshGene Expression Regulation, Developmental - Drug Effectsen_US
dc.subject.meshGene Expression Regulation, Enzymologic - Drug Effectsen_US
dc.subject.meshGene Expression Regulation, Plant - Drug Effectsen_US
dc.subject.meshHydroxymethylglutaryl-Coa Synthase - Chemistry - Geneticsen_US
dc.subject.meshMannitol - Pharmacologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMultigene Familyen_US
dc.subject.meshRecombinant Proteins - Chemistry - Geneticsen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.titleExpression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsiveen_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1046/j.1365-313X.2000.00751.xen_US
dc.identifier.pmid10849357-
dc.identifier.scopuseid_2-s2.0-0034124423en_US
dc.identifier.hkuros48812-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034124423&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume22en_US
dc.identifier.issue5en_US
dc.identifier.spage415en_US
dc.identifier.epage426en_US
dc.identifier.isiWOS:000087312200004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridAlex, D=55255924500en_US
dc.identifier.scopusauthoridBach, TJ=24786011400en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US

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