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Article: Effect of polysaccharide krestin on glutathione peroxidase gene expression in mouse peritoneal macrophages

TitleEffect of polysaccharide krestin on glutathione peroxidase gene expression in mouse peritoneal macrophages
Authors
Issue Date2000
PublisherStep Communications Ltd. The Journal's web site is located at http://www.ibms.org/index.cfm?method=publications.british_journal
Citation
British Journal Of Biomedical Science, 2000, v. 57 n. 2, p. 130-136 How to Cite?
AbstractPolysaccharide krestin (PSK) is a protein-bound polysaccharide extracted from the sporophore Coriolus versicolor. Previously, we found that PSK could reduce the oxidative injury that oxidised low-density lipoprotein (Ox-LDL) produced in monocytes/macrophages, and therefore have some prophylactic or therapeutic effect on atherosclerosis. Glutathione peroxidases, including selenium-dependent glutathione peroxidase (SeGPx) and nonselenium-dependent glutathione peroxidase (non-SeGPx, also called glutathione S-transferase [GST]), play an important role in the defence against oxidative injury. In order to find out if the effects of PSK were associated with antioxidant enzymes, we investigated its effect on glutathione peroxidase activity and messenger RNA (mRNA) expression in mouse peritoneal macrophages. Results showed that PSK enhanced SeGPx and non-SeGPx activity, and increased SeGPx and GST-P (π class GST) mRNA in mouse peritoneal macrophages. In addition, the induction by PSK of the two glutathione peroxidases could be blocked by cycloheximide (30 μg/mL), but 5 μg/mL actinomycin D and 50 μg/mL acetovanilone (a superoxide inhibitor) had no effect. We conclude that PSK improved glutathione peroxidase activity through transcriptional induction of mRNA expression.
Persistent Identifierhttp://hdl.handle.net/10722/178707
ISSN
2015 Impact Factor: 1.245
2015 SCImago Journal Rankings: 0.352
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPang, ZJen_US
dc.contributor.authorChen, Yen_US
dc.contributor.authorZhou, Men_US
dc.contributor.authorWan, Jen_US
dc.date.accessioned2012-12-19T09:49:15Z-
dc.date.available2012-12-19T09:49:15Z-
dc.date.issued2000en_US
dc.identifier.citationBritish Journal Of Biomedical Science, 2000, v. 57 n. 2, p. 130-136en_US
dc.identifier.issn0967-4845en_US
dc.identifier.urihttp://hdl.handle.net/10722/178707-
dc.description.abstractPolysaccharide krestin (PSK) is a protein-bound polysaccharide extracted from the sporophore Coriolus versicolor. Previously, we found that PSK could reduce the oxidative injury that oxidised low-density lipoprotein (Ox-LDL) produced in monocytes/macrophages, and therefore have some prophylactic or therapeutic effect on atherosclerosis. Glutathione peroxidases, including selenium-dependent glutathione peroxidase (SeGPx) and nonselenium-dependent glutathione peroxidase (non-SeGPx, also called glutathione S-transferase [GST]), play an important role in the defence against oxidative injury. In order to find out if the effects of PSK were associated with antioxidant enzymes, we investigated its effect on glutathione peroxidase activity and messenger RNA (mRNA) expression in mouse peritoneal macrophages. Results showed that PSK enhanced SeGPx and non-SeGPx activity, and increased SeGPx and GST-P (π class GST) mRNA in mouse peritoneal macrophages. In addition, the induction by PSK of the two glutathione peroxidases could be blocked by cycloheximide (30 μg/mL), but 5 μg/mL actinomycin D and 50 μg/mL acetovanilone (a superoxide inhibitor) had no effect. We conclude that PSK improved glutathione peroxidase activity through transcriptional induction of mRNA expression.en_US
dc.languageengen_US
dc.publisherStep Communications Ltd. The Journal's web site is located at http://www.ibms.org/index.cfm?method=publications.british_journalen_US
dc.relation.ispartofBritish Journal of Biomedical Scienceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibiotics, Antineoplastic - Pharmacologyen_US
dc.subject.meshGene Expression Regulation, Enzymologic - Drug Effectsen_US
dc.subject.meshGlutathione Peroxidase - Drug Effects - Genetics - Metabolismen_US
dc.subject.meshImmunologic Factors - Pharmacologyen_US
dc.subject.meshMacrophages, Peritoneal - Drug Effects - Enzymologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshProteoglycans - Pharmacologyen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.titleEffect of polysaccharide krestin on glutathione peroxidase gene expression in mouse peritoneal macrophagesen_US
dc.typeArticleen_US
dc.identifier.emailWan, J: jmfwan@hku.hken_US
dc.identifier.authorityWan, J=rp00798en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10912287-
dc.identifier.scopuseid_2-s2.0-0034047882en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034047882&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume57en_US
dc.identifier.issue2en_US
dc.identifier.spage130en_US
dc.identifier.epage136en_US
dc.identifier.isiWOS:000087833700005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridPang, ZJ=7103343225en_US
dc.identifier.scopusauthoridChen, Y=16745998900en_US
dc.identifier.scopusauthoridZhou, M=7403506134en_US
dc.identifier.scopusauthoridWan, J=8930305000en_US

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