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Article: Toxic effects of cadmium on fertilizing capability of spermatozoa, dynamics of the first cleavage and pluteus formation in the sea urchin Anthocidaris crassispina (Agassiz)

TitleToxic effects of cadmium on fertilizing capability of spermatozoa, dynamics of the first cleavage and pluteus formation in the sea urchin Anthocidaris crassispina (Agassiz)
Authors
KeywordsAnthocidaris Crassispina
Cleavage Dynamics
Fertilization
Pluteus Formation
Sea Urchin
Spermatozoa
Issue Date1999
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
Citation
Marine Pollution Bulletin, 1999, v. 38 n. 12, p. 1097-1104 How to Cite?
AbstractEffects of cadmium on the fertilizing capability of spermatozoa, dynamics of the first cleavage and pluteus formation in the sea urchin, Anthocidaris crassispina, were investigated. Exposure to cadmium concentrations of 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10.0 mg l-1 for 30 min caused a significant adverse effect on the fertilizing capability of spermatozoa (EC50=1.7 mg l-1). For the first cleavage kinetics assay, eggs were pre-exposed to 0, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2 and 5.6 mg l-1 of cadmium for 30 min. Fresh sperms of fixed concentrations were then added to the eggs and the percentage of divided zygotes estimated. Concentration-dependent toxic effects on the dynamics of the first cleavage in A. crassispina were observed between 0.18 and 5.6 mg l-1 cadmium. Calculation of EC50 values by probit analysis at different stages of the first cleavage revealed that EC50 values increased gradually from 0.37 mg l-1 at the beginning to 2.24 mg l-1 at the end of the cleavage process. Different larval classes (normal, abnormal and retarded plutei, preplutei, dead embryos/larvae) of A. crassispina obtained from eggs pre-treated with cadmium for 30 min and then exposed to cadmium throughout embryogenesis were also examined. The percentage of abnormal plutei was significantly higher than that of the control at 0.56 mg l-1 cadmium and higher. The 48 h EC50 calculated for the number of normal plutei was 1.5 mg l-1. A χ2-test revealed significant changes in the distribution of proportions of different larval classes only at cadmium concentrations ≥1.8 mg l-1. At 5.6 mg l-1 of cadmium, small malformed plutei, and oval embryos with rudimentary skeleton, poorly differentiated intestines and no mouth (pre-pluteus stage) were observed. The relative sensitivity of various end points to cadmium is also compared and discussed. Copyright (C) 1999 Elsevier Science Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/178683
ISSN
2015 Impact Factor: 3.099
2015 SCImago Journal Rankings: 1.264
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorVaschenko, MAen_US
dc.contributor.authorZhang, ZPen_US
dc.contributor.authorLam, PKSen_US
dc.contributor.authorWu, RSSen_US
dc.date.accessioned2012-12-19T09:49:07Z-
dc.date.available2012-12-19T09:49:07Z-
dc.date.issued1999en_US
dc.identifier.citationMarine Pollution Bulletin, 1999, v. 38 n. 12, p. 1097-1104en_US
dc.identifier.issn0025-326Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/178683-
dc.description.abstractEffects of cadmium on the fertilizing capability of spermatozoa, dynamics of the first cleavage and pluteus formation in the sea urchin, Anthocidaris crassispina, were investigated. Exposure to cadmium concentrations of 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10.0 mg l-1 for 30 min caused a significant adverse effect on the fertilizing capability of spermatozoa (EC50=1.7 mg l-1). For the first cleavage kinetics assay, eggs were pre-exposed to 0, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2 and 5.6 mg l-1 of cadmium for 30 min. Fresh sperms of fixed concentrations were then added to the eggs and the percentage of divided zygotes estimated. Concentration-dependent toxic effects on the dynamics of the first cleavage in A. crassispina were observed between 0.18 and 5.6 mg l-1 cadmium. Calculation of EC50 values by probit analysis at different stages of the first cleavage revealed that EC50 values increased gradually from 0.37 mg l-1 at the beginning to 2.24 mg l-1 at the end of the cleavage process. Different larval classes (normal, abnormal and retarded plutei, preplutei, dead embryos/larvae) of A. crassispina obtained from eggs pre-treated with cadmium for 30 min and then exposed to cadmium throughout embryogenesis were also examined. The percentage of abnormal plutei was significantly higher than that of the control at 0.56 mg l-1 cadmium and higher. The 48 h EC50 calculated for the number of normal plutei was 1.5 mg l-1. A χ2-test revealed significant changes in the distribution of proportions of different larval classes only at cadmium concentrations ≥1.8 mg l-1. At 5.6 mg l-1 of cadmium, small malformed plutei, and oval embryos with rudimentary skeleton, poorly differentiated intestines and no mouth (pre-pluteus stage) were observed. The relative sensitivity of various end points to cadmium is also compared and discussed. Copyright (C) 1999 Elsevier Science Ltd.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbulen_US
dc.relation.ispartofMarine Pollution Bulletinen_US
dc.subjectAnthocidaris Crassispinaen_US
dc.subjectCleavage Dynamicsen_US
dc.subjectFertilizationen_US
dc.subjectPluteus Formationen_US
dc.subjectSea Urchinen_US
dc.subjectSpermatozoaen_US
dc.titleToxic effects of cadmium on fertilizing capability of spermatozoa, dynamics of the first cleavage and pluteus formation in the sea urchin Anthocidaris crassispina (Agassiz)en_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0025-326X(99)00116-2en_US
dc.identifier.scopuseid_2-s2.0-0033377920en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033377920&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume38en_US
dc.identifier.issue12en_US
dc.identifier.spage1097en_US
dc.identifier.epage1104en_US
dc.identifier.isiWOS:000084587100017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridVaschenko, MA=6603014054en_US
dc.identifier.scopusauthoridZhang, ZP=15040750500en_US
dc.identifier.scopusauthoridLam, PKS=7202365776en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US

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