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Article: cDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum

TitlecDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum
Authors
Issue Date1999
PublisherAmerican Phytopathological Society. The Journal's web site is located at http://www.apsnet.org/mpmi
Citation
Molecular Plant-Microbe Interactions, 1999, v. 12 n. 6, p. 479-489 How to Cite?
AbstractA sorghum cDNA clone was isolated by differential screening of a cDNA library prepared from mesocotyls (cultivar DK18) inoculated with fungal pathogens. The deduced translation product shows sequence similarity to a family of intracellular pathogenesis-related proteins (PR-10) with a potential ribonuclease function. We studied the accumulation of PR-10 and chalcone synthase (CHS) transcripts in mesocotyls following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum. CHS is involved in phytoalexin synthesis in sorghum. Coordinate expression of PR-10 and CHS genes was localized in the area of inoculation along with the accumulation of phytoalexins. C. heterostrophus is a nonpathogen of sorghum and cytological studies indicated that cultivar DK18 is resistant to C. sublineolum, a sorghum pathogen. We demonstrated that the two fungi triggered different time courses of plant defense reactions. Inoculation with C. heterostrophus resulted in rapid accumulation of PR-10 and CHS transcripts after appressoria had become mature. Accumulation of these transcripts was delayed in plants inoculated with C. sublineolum until penetration of host tissue had been completed and infection vesicles had formed. Results suggest that different recognition events are involved in the expression of resistance to the two fungi used or that C. sublineolum suppresses the nonspecific induction of defense responses.
Persistent Identifierhttp://hdl.handle.net/10722/178676
ISSN
2015 Impact Factor: 4.145
2015 SCImago Journal Rankings: 2.196
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, SCCen_US
dc.contributor.authorHipskind, JDen_US
dc.contributor.authorNicholson, RLen_US
dc.date.accessioned2012-12-19T09:49:04Z-
dc.date.available2012-12-19T09:49:04Z-
dc.date.issued1999en_US
dc.identifier.citationMolecular Plant-Microbe Interactions, 1999, v. 12 n. 6, p. 479-489en_US
dc.identifier.issn0894-0282en_US
dc.identifier.urihttp://hdl.handle.net/10722/178676-
dc.description.abstractA sorghum cDNA clone was isolated by differential screening of a cDNA library prepared from mesocotyls (cultivar DK18) inoculated with fungal pathogens. The deduced translation product shows sequence similarity to a family of intracellular pathogenesis-related proteins (PR-10) with a potential ribonuclease function. We studied the accumulation of PR-10 and chalcone synthase (CHS) transcripts in mesocotyls following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum. CHS is involved in phytoalexin synthesis in sorghum. Coordinate expression of PR-10 and CHS genes was localized in the area of inoculation along with the accumulation of phytoalexins. C. heterostrophus is a nonpathogen of sorghum and cytological studies indicated that cultivar DK18 is resistant to C. sublineolum, a sorghum pathogen. We demonstrated that the two fungi triggered different time courses of plant defense reactions. Inoculation with C. heterostrophus resulted in rapid accumulation of PR-10 and CHS transcripts after appressoria had become mature. Accumulation of these transcripts was delayed in plants inoculated with C. sublineolum until penetration of host tissue had been completed and infection vesicles had formed. Results suggest that different recognition events are involved in the expression of resistance to the two fungi used or that C. sublineolum suppresses the nonspecific induction of defense responses.en_US
dc.languageengen_US
dc.publisherAmerican Phytopathological Society. The Journal's web site is located at http://www.apsnet.org/mpmien_US
dc.relation.ispartofMolecular Plant-Microbe Interactionsen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAscomycota - Pathogenicityen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCereals - Genetics - Microbiologyen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna, Complementaryen_US
dc.subject.meshGene Expression Regulation, Planten_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlant Proteins - Biosynthesis - Chemistry - Geneticsen_US
dc.subject.meshPlants - Geneticsen_US
dc.subject.meshRecombinant Proteins - Biosynthesis - Chemistryen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.titlecDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolumen_US
dc.typeArticleen_US
dc.identifier.emailLo, SCC: clivelo@hkucc.hku.hken_US
dc.identifier.authorityLo, SCC=rp00751en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1094/MPMI.1999.12.6.479-
dc.identifier.pmid10356799-
dc.identifier.scopuseid_2-s2.0-0033151721en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033151721&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume12en_US
dc.identifier.issue6en_US
dc.identifier.spage479en_US
dc.identifier.epage489en_US
dc.identifier.isiWOS:000080383500001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLo, SCC=15737175700en_US
dc.identifier.scopusauthoridHipskind, JD=6603397831en_US
dc.identifier.scopusauthoridNicholson, RL=7201624684en_US

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