File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Production of recombinant goldfish growth hormone I in a baculovirus expression system.

TitleProduction of recombinant goldfish growth hormone I in a baculovirus expression system.
Authors
Issue Date1997
PublisherAllerton Press, Inc.
Citation
Chinese Journal Of Biotechnology, 1997, v. 13 n. 2, p. 91-97 How to Cite?
AbstractThe cDNA sequence encoding the growth hormone (GH) I gene of goldfish (gf GH-I) was cloned into the pSXIVVI+ X3 baculovirus transfer vector. This transfer vector, without the initiation codon (ATG) and using a SynXIV promoter to activate transcription, was constructed for a baculovirus expression system. The recombinant transfer vector with the gf GH-I insert was used to produce the recombinant baculovirus TnNPV-SX+ gf GH-I 21a. Both in vivo and in vitro approaches were used to test the expression of growth hormone I gene using TnNPV-SX+ gf GH-I 21a. For in vivo studies, larvae of Trichoplusia ni were infected with the recombinant baculovirus. Four days later, growth hormone-like immunoreactivity was detected in the hemolymph of these infected larvae. For in vitro studies, insect Spodoptera frugiperda 9(Sf9) cells were infected with TnNPV-SX+ gf GH-I 21a. After incubation for at least 24 hours, growth hormone-like immunoreactivity was detected in Sf9 insect cells as well as in the culture medium. Western blotting analysis of larval hemolymph and Sf9 cell contents after viral infection revealed a protein band of 22.5 kDa immunoreactive to goldfish growth hormone antiserum. This is consistent with the predicted molecular weight deduced from the cDNA of goldfish growth hormone I gene. These results, taken together, suggest that the baculovirus expression system can be used to produce the recombinant growth hormone of a fish species.
Persistent Identifierhttp://hdl.handle.net/10722/178611
ISSN
2011 SCImago Journal Rankings: 0.270

 

DC FieldValueLanguage
dc.contributor.authorLin, Gen_US
dc.contributor.authorWang, Xen_US
dc.contributor.authorLong, Qen_US
dc.contributor.authorPang, Yen_US
dc.contributor.authorWong, AOen_US
dc.contributor.authorYu, Ken_US
dc.date.accessioned2012-12-19T09:48:41Z-
dc.date.available2012-12-19T09:48:41Z-
dc.date.issued1997en_US
dc.identifier.citationChinese Journal Of Biotechnology, 1997, v. 13 n. 2, p. 91-97en_US
dc.identifier.issn1042-749Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/178611-
dc.description.abstractThe cDNA sequence encoding the growth hormone (GH) I gene of goldfish (gf GH-I) was cloned into the pSXIVVI+ X3 baculovirus transfer vector. This transfer vector, without the initiation codon (ATG) and using a SynXIV promoter to activate transcription, was constructed for a baculovirus expression system. The recombinant transfer vector with the gf GH-I insert was used to produce the recombinant baculovirus TnNPV-SX+ gf GH-I 21a. Both in vivo and in vitro approaches were used to test the expression of growth hormone I gene using TnNPV-SX+ gf GH-I 21a. For in vivo studies, larvae of Trichoplusia ni were infected with the recombinant baculovirus. Four days later, growth hormone-like immunoreactivity was detected in the hemolymph of these infected larvae. For in vitro studies, insect Spodoptera frugiperda 9(Sf9) cells were infected with TnNPV-SX+ gf GH-I 21a. After incubation for at least 24 hours, growth hormone-like immunoreactivity was detected in Sf9 insect cells as well as in the culture medium. Western blotting analysis of larval hemolymph and Sf9 cell contents after viral infection revealed a protein band of 22.5 kDa immunoreactive to goldfish growth hormone antiserum. This is consistent with the predicted molecular weight deduced from the cDNA of goldfish growth hormone I gene. These results, taken together, suggest that the baculovirus expression system can be used to produce the recombinant growth hormone of a fish species.en_US
dc.languageengen_US
dc.publisherAllerton Press, Inc.-
dc.relation.ispartofChinese journal of biotechnologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshDeoxyribonucleases, Type Ii Site-Specific - Metabolismen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGenetic Vectorsen_US
dc.subject.meshGoldfishen_US
dc.subject.meshGrowth Hormone - Biosynthesis - Geneticsen_US
dc.subject.meshLarvaen_US
dc.subject.meshMothsen_US
dc.subject.meshNucleopolyhedrovirusen_US
dc.subject.meshSpodoptera - Cytologyen_US
dc.titleProduction of recombinant goldfish growth hormone I in a baculovirus expression system.en_US
dc.typeArticleen_US
dc.identifier.emailWong, AO: olwong@hkucc.hku.hken_US
dc.identifier.authorityWong, AO=rp00806en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9343707-
dc.identifier.scopuseid_2-s2.0-0031308337en_US
dc.identifier.hkuros36756-
dc.identifier.volume13en_US
dc.identifier.issue2en_US
dc.identifier.spage91en_US
dc.identifier.epage97en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLin, G=7401699870en_US
dc.identifier.scopusauthoridWang, X=7501867188en_US
dc.identifier.scopusauthoridLong, Q=7005631111en_US
dc.identifier.scopusauthoridPang, Y=7201685883en_US
dc.identifier.scopusauthoridWong, AO=7403147570en_US
dc.identifier.scopusauthoridYu, K=55231884000en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats