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Article: Expression of the carbohydrate recognition domain of lung surfactant protein D and demonstration of its binding to lipopolysaccharides of Gram-negative bacteria

TitleExpression of the carbohydrate recognition domain of lung surfactant protein D and demonstration of its binding to lipopolysaccharides of Gram-negative bacteria
Authors
Issue Date1994
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1994, v. 202 n. 3, p. 1674-1680 How to Cite?
AbstractSurfactant protein D is a collagenous C-type lectin (collectin) that is found almost exclusively in the lung. A recombinant protein, composed of the neck-region and the carbohydrate binding domain of bovine lung surfactant protein D, has been overexpressed in E. coli. The recombinant protein showed the same sugar binding specificity as the native protein and was able to bind to the lipopolysaccharides of several strains of Gram-negative bacteria, such as Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, which are known to cause lung infections. The binding was calcium-dependent and was inhibited by maltose. Native bovine surfactant protein D was also shown to be able to bind to these lipopolysaccharides in the same manner.
Persistent Identifierhttp://hdl.handle.net/10722/178560
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLim, BLen_US
dc.contributor.authorWang, JYen_US
dc.contributor.authorHolmskov, Uen_US
dc.contributor.authorHoppe, HJen_US
dc.contributor.authorReid, KBMen_US
dc.date.accessioned2012-12-19T09:48:23Z-
dc.date.available2012-12-19T09:48:23Z-
dc.date.issued1994en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 1994, v. 202 n. 3, p. 1674-1680en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/178560-
dc.description.abstractSurfactant protein D is a collagenous C-type lectin (collectin) that is found almost exclusively in the lung. A recombinant protein, composed of the neck-region and the carbohydrate binding domain of bovine lung surfactant protein D, has been overexpressed in E. coli. The recombinant protein showed the same sugar binding specificity as the native protein and was able to bind to the lipopolysaccharides of several strains of Gram-negative bacteria, such as Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, which are known to cause lung infections. The binding was calcium-dependent and was inhibited by maltose. Native bovine surfactant protein D was also shown to be able to bind to these lipopolysaccharides in the same manner.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCarbohydrate Metabolismen_US
dc.subject.meshCattleen_US
dc.subject.meshEscherichia Coli - Metabolismen_US
dc.subject.meshGlutathione Transferase - Metabolismen_US
dc.subject.meshGlycoproteins - Genetics - Metabolismen_US
dc.subject.meshKlebsiella Pneumoniae - Metabolismen_US
dc.subject.meshLipopolysaccharides - Metabolismen_US
dc.subject.meshPseudomonas Aeruginosa - Metabolismen_US
dc.subject.meshPulmonary Surfactant-Associated Protein Den_US
dc.subject.meshPulmonary Surfactants - Genetics - Metabolismen_US
dc.subject.meshRecombinant Fusion Proteins - Metabolismen_US
dc.titleExpression of the carbohydrate recognition domain of lung surfactant protein D and demonstration of its binding to lipopolysaccharides of Gram-negative bacteriaen_US
dc.typeArticleen_US
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_US
dc.identifier.authorityLim, BL=rp00744en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/bbrc.1994.2127en_US
dc.identifier.pmid8060356-
dc.identifier.scopuseid_2-s2.0-0028023398en_US
dc.identifier.hkuros9693-
dc.identifier.volume202en_US
dc.identifier.issue3en_US
dc.identifier.spage1674en_US
dc.identifier.epage1680en_US
dc.identifier.isiWOS:A1994PB55300071-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLim, BL=7201983917en_US
dc.identifier.scopusauthoridWang, JY=8089862700en_US
dc.identifier.scopusauthoridHolmskov, U=7004526416en_US
dc.identifier.scopusauthoridHoppe, HJ=7103057361en_US
dc.identifier.scopusauthoridReid, KBM=7202780648en_US

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