File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Three genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed

TitleThree genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed
Authors
Issue Date1992
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 1992, v. 19 n. 3, p. 473-484 How to Cite?
AbstractThe enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyses an important step in isoprenoid biosynthesis in plants. In Hevea brasiliensis, HMGR is encoded by a small gene family comprised of three members, hmg1, hmg2 and hmg3. We have previously described hmg1 and hmg2 (Plant Mol Biol 16: 567-577, 1991). Here we report the isolation and characterization of hmg3 genomic and cDNA clones. In comparison to hmg1 which is more highly expressed in laticifers than in leaves, the level of hmg3 mRNA level is equally abundant in laticifers and leaves. In situ hybridization experiments showed that the expression of hmg3 is not cell-type specific while hmg1 is expressed predominantly in the laticifers. Primer-extension experiments using laticifer RNA showed that hmg1 is induced by ethylene while hmg3 expression remains constitutive. The hmg3 promoter, like the promoters of most house-keeping genes, lacks a TATA box. Our results suggest that hmg1 is likely to encode the enzyme involved in rubber biosynthesis while hmg3 is possibly involved in isoprenoid biosynthesis of a housekeeping nature. © 1992 Kluwer Academic Publishers.
Persistent Identifierhttp://hdl.handle.net/10722/178534
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChye, MLen_US
dc.contributor.authorTan, CTen_US
dc.contributor.authorChua, NHen_US
dc.date.accessioned2012-12-19T09:48:15Z-
dc.date.available2012-12-19T09:48:15Z-
dc.date.issued1992en_US
dc.identifier.citationPlant Molecular Biology, 1992, v. 19 n. 3, p. 473-484en_US
dc.identifier.issn0167-4412en_US
dc.identifier.urihttp://hdl.handle.net/10722/178534-
dc.description.abstractThe enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyses an important step in isoprenoid biosynthesis in plants. In Hevea brasiliensis, HMGR is encoded by a small gene family comprised of three members, hmg1, hmg2 and hmg3. We have previously described hmg1 and hmg2 (Plant Mol Biol 16: 567-577, 1991). Here we report the isolation and characterization of hmg3 genomic and cDNA clones. In comparison to hmg1 which is more highly expressed in laticifers than in leaves, the level of hmg3 mRNA level is equally abundant in laticifers and leaves. In situ hybridization experiments showed that the expression of hmg3 is not cell-type specific while hmg1 is expressed predominantly in the laticifers. Primer-extension experiments using laticifer RNA showed that hmg1 is induced by ethylene while hmg3 expression remains constitutive. The hmg3 promoter, like the promoters of most house-keeping genes, lacks a TATA box. Our results suggest that hmg1 is likely to encode the enzyme involved in rubber biosynthesis while hmg3 is possibly involved in isoprenoid biosynthesis of a housekeeping nature. © 1992 Kluwer Academic Publishers.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_US
dc.relation.ispartofPlant Molecular Biologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna - Genetics - Isolation & Purificationen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGenes, Planten_US
dc.subject.meshGenomic Libraryen_US
dc.subject.meshHydroxymethylglutaryl Coa Reductases - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligodeoxyribonucleotidesen_US
dc.subject.meshPlant Cellsen_US
dc.subject.meshPlants - Enzymology - Geneticsen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProtein Conformationen_US
dc.subject.meshRna - Genetics - Isolation & Purificationen_US
dc.subject.meshRna, Messenger - Analysis - Geneticsen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.titleThree genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expresseden_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00023395en_US
dc.identifier.pmid1377968-
dc.identifier.scopuseid_2-s2.0-0026871155en_US
dc.identifier.volume19en_US
dc.identifier.issue3en_US
dc.identifier.spage473en_US
dc.identifier.epage484en_US
dc.identifier.isiWOS:A1992JA98800013-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US
dc.identifier.scopusauthoridTan, CT=7402818170en_US
dc.identifier.scopusauthoridChua, NH=7103077967en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats