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Article: Molecular biology of the 2-haloacid halidohydrolase IVa from Pseudomonas cepacia MBA4

TitleMolecular biology of the 2-haloacid halidohydrolase IVa from Pseudomonas cepacia MBA4
Authors
Issue Date1992
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 1992, v. 284 n. 1, p. 87-93 How to Cite?
AbstractThe structural gene (hdl IVa) for the Pseudomonas cepacia MBA4 2-haloacid halidohydrolase IVa (Hdl IVa) was isolated on a 1.6kb fragment of Ps. cepacia MBA4 chromosomal DNA. The recombinant halidohydrolase was expressed in Escherichia coli and Pseudomonas putida and the structural gene was subcloned on to the tac expression vector pBTac1. High-level expression from the tac promoter was seen to be temperature-dependent, a consequence of the nucleotide sequence adjacent to the fragment encoding the halidohydrolase. The nucleotide sequence of the fragment encoding the Hdl IVa was determined and analysed. Three ATG codons were identified in one of the open reading frames and the one corresponding to the start of the hdl IVa structural gene was determined by comparison of the predicted amino acid sequences with the experimentally determined N-terminal sequences of halidohydrolase IVa. The hdl IVa gene encoded a 231 amino acid-residue protein of M(r) 25 900. The sequence and predicted structural data are discussed and comparison is made with sequence data for other halidohydrolases.
Persistent Identifierhttp://hdl.handle.net/10722/178526
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMurdiyatmo, Uen_US
dc.contributor.authorAsmara, Wen_US
dc.contributor.authorTsang, JSHen_US
dc.contributor.authorBaines, AJen_US
dc.contributor.authorBull, ATen_US
dc.contributor.authorHardman, DJen_US
dc.date.accessioned2012-12-19T09:48:13Z-
dc.date.available2012-12-19T09:48:13Z-
dc.date.issued1992en_US
dc.identifier.citationBiochemical Journal, 1992, v. 284 n. 1, p. 87-93en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/178526-
dc.description.abstractThe structural gene (hdl IVa) for the Pseudomonas cepacia MBA4 2-haloacid halidohydrolase IVa (Hdl IVa) was isolated on a 1.6kb fragment of Ps. cepacia MBA4 chromosomal DNA. The recombinant halidohydrolase was expressed in Escherichia coli and Pseudomonas putida and the structural gene was subcloned on to the tac expression vector pBTac1. High-level expression from the tac promoter was seen to be temperature-dependent, a consequence of the nucleotide sequence adjacent to the fragment encoding the halidohydrolase. The nucleotide sequence of the fragment encoding the Hdl IVa was determined and analysed. Three ATG codons were identified in one of the open reading frames and the one corresponding to the start of the hdl IVa structural gene was determined by comparison of the predicted amino acid sequences with the experimentally determined N-terminal sequences of halidohydrolase IVa. The hdl IVa gene encoded a 231 amino acid-residue protein of M(r) 25 900. The sequence and predicted structural data are discussed and comparison is made with sequence data for other halidohydrolases.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBiological Evolutionen_US
dc.subject.meshBurkholderia Cepacia - Enzymology - Geneticsen_US
dc.subject.meshEscherichia Coli - Enzymology - Geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGenes, Bacterial - Geneticsen_US
dc.subject.meshHydrolases - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPseudomonas Putida - Enzymology - Geneticsen_US
dc.titleMolecular biology of the 2-haloacid halidohydrolase IVa from Pseudomonas cepacia MBA4en_US
dc.typeArticleen_US
dc.identifier.emailTsang, JSH: jshtsang@hku.hken_US
dc.identifier.authorityTsang, JSH=rp00792en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1376111-
dc.identifier.scopuseid_2-s2.0-0026555590en_US
dc.identifier.volume284en_US
dc.identifier.issue1en_US
dc.identifier.spage87en_US
dc.identifier.epage93en_US
dc.identifier.isiWOS:A1992HV22300013-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridMurdiyatmo, U=6507008521en_US
dc.identifier.scopusauthoridAsmara, W=6506675601en_US
dc.identifier.scopusauthoridTsang, JSH=7102483508en_US
dc.identifier.scopusauthoridBaines, AJ=23046437500en_US
dc.identifier.scopusauthoridBull, AT=24538530400en_US
dc.identifier.scopusauthoridHardman, DJ=7004649669en_US

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