File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: High density cultivation of BSK cells on sintered alumina ceramic foam support

TitleHigh density cultivation of BSK cells on sintered alumina ceramic foam support
Authors
Issue Date1991
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-9069
Citation
Cytotechnology, 1991, v. 5 n. 3, p. 233-241 How to Cite?
AbstractA perfusion system which utilizes a porous ceramic core has been tested for the cultivation of transformed BHK cells which produce human transferrin. A design is presented in which cells are immobilized within the porous ceramic particle and are fed by continuous perfusion of batch liquid medium. It was found that more than 5×109 BHK cells could be supported within the 40 mL ceramic matrix, a ten-fold increase in cell density per unit surface area over the standard roller bottle cultures or a five-fold increase in volumetric cell density over suspension cultures. The cell specific productivity of human transferrin was similar to that observed in suspension culture. The system offers the advantages of significant reduction in serum requirements and the potential for scale-up. © 1991 Kluwer Academic Publishers.
Persistent Identifierhttp://hdl.handle.net/10722/178515
ISSN
2015 Impact Factor: 1.864
2015 SCImago Journal Rankings: 0.525
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, DWen_US
dc.contributor.authorGrace, JRen_US
dc.contributor.authorChow, BKCen_US
dc.contributor.authorMacgillivray, RTAen_US
dc.contributor.authorKilburn, DGen_US
dc.date.accessioned2012-12-19T09:48:09Z-
dc.date.available2012-12-19T09:48:09Z-
dc.date.issued1991en_US
dc.identifier.citationCytotechnology, 1991, v. 5 n. 3, p. 233-241en_US
dc.identifier.issn0920-9069en_US
dc.identifier.urihttp://hdl.handle.net/10722/178515-
dc.description.abstractA perfusion system which utilizes a porous ceramic core has been tested for the cultivation of transformed BHK cells which produce human transferrin. A design is presented in which cells are immobilized within the porous ceramic particle and are fed by continuous perfusion of batch liquid medium. It was found that more than 5×109 BHK cells could be supported within the 40 mL ceramic matrix, a ten-fold increase in cell density per unit surface area over the standard roller bottle cultures or a five-fold increase in volumetric cell density over suspension cultures. The cell specific productivity of human transferrin was similar to that observed in suspension culture. The system offers the advantages of significant reduction in serum requirements and the potential for scale-up. © 1991 Kluwer Academic Publishers.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-9069en_US
dc.relation.ispartofCytotechnologyen_US
dc.subject.meshAluminum Oxideen_US
dc.subject.meshCell Counten_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCeramicsen_US
dc.subject.meshCloning, Molecular - Methodsen_US
dc.subject.meshGlucose - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshKineticsen_US
dc.subject.meshLactates - Biosynthesisen_US
dc.subject.meshOxygen Consumptionen_US
dc.subject.meshPerfusionen_US
dc.subject.meshPorosityen_US
dc.subject.meshTransferrin - Biosynthesisen_US
dc.titleHigh density cultivation of BSK cells on sintered alumina ceramic foam supporten_US
dc.typeArticleen_US
dc.identifier.emailChow, BKC: bkcc@hku.hken_US
dc.identifier.authorityChow, BKC=rp00681en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00556293en_US
dc.identifier.pmid1367376en_US
dc.identifier.scopuseid_2-s2.0-0026129638en_US
dc.identifier.volume5en_US
dc.identifier.issue3en_US
dc.identifier.spage233en_US
dc.identifier.epage241en_US
dc.identifier.isiWOS:A1991FN53400006-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLee, DW=16151945000en_US
dc.identifier.scopusauthoridGrace, JR=7202870990en_US
dc.identifier.scopusauthoridChow, BKC=7102826193en_US
dc.identifier.scopusauthoridMacGillivray, RTA=7004286039en_US
dc.identifier.scopusauthoridKilburn, DG=35500772400en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats