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Article: Circadian rhythms of melatonin release from chicken pineal in vitro: Modified melatonin radioimmunoassay

TitleCircadian rhythms of melatonin release from chicken pineal in vitro: Modified melatonin radioimmunoassay
Authors
Issue Date1991
PublisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/
Citation
Proceedings Of The Society For Experimental Biology And Medicine, 1991, v. 198 n. 3, p. 826-832 How to Cite?
AbstractAn improved and simplified radioimmunoassay for measuring pineal, serum, and in vitro cultured medium melatonin is described. Using 2- [125I]iodomelatonin as radiolabeled ligand and a polyclonal rabbit antimelatonin antiserum, melatonin concentrations were determined in all three types of samples by a 2-day direct equilibrium double-antibody assay method without prior extraction. Serial dilutions of pineal homogenates, serum, and cultured medium all gave parallel displacement curves. Cross- reactivity of the antisera with other indoles was negligible. Intraassay coefficients of variation (n = 3) were 5.09, 3.32, and 5.05% at 7.81, 62.5, and 500 pg/tube, respectively, and the interassay coefficients of variation (n = 20) were 12.18% at 62.5 pg/tube. A characteristic diurnal rhythm of melatonin was observed using this direct assay for measuring daytime and nighttime chicken pineal and serum samples. An in vitro incubation of chicken pineal glands with a lighting cycle of 12-hr light: 12-hr dark showed that the diurnal rhythm of melatonin secretion into the cultured medium was maintained. The direct assay method described in this report for measuring chicken melatonin using 2-[125I]iodomelatonin as radiolabeled ligand coupled with the in vitro cultured chicken pineal gland clearly offers great potential for studying the chicken pineal circadian oscillator and its underlying mechanism.
Persistent Identifierhttp://hdl.handle.net/10722/178513
ISSN
2002 Impact Factor: 2.714
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:48:09Z-
dc.date.available2012-12-19T09:48:09Z-
dc.date.issued1991en_US
dc.identifier.citationProceedings Of The Society For Experimental Biology And Medicine, 1991, v. 198 n. 3, p. 826-832en_US
dc.identifier.issn0037-9727en_US
dc.identifier.urihttp://hdl.handle.net/10722/178513-
dc.description.abstractAn improved and simplified radioimmunoassay for measuring pineal, serum, and in vitro cultured medium melatonin is described. Using 2- [125I]iodomelatonin as radiolabeled ligand and a polyclonal rabbit antimelatonin antiserum, melatonin concentrations were determined in all three types of samples by a 2-day direct equilibrium double-antibody assay method without prior extraction. Serial dilutions of pineal homogenates, serum, and cultured medium all gave parallel displacement curves. Cross- reactivity of the antisera with other indoles was negligible. Intraassay coefficients of variation (n = 3) were 5.09, 3.32, and 5.05% at 7.81, 62.5, and 500 pg/tube, respectively, and the interassay coefficients of variation (n = 20) were 12.18% at 62.5 pg/tube. A characteristic diurnal rhythm of melatonin was observed using this direct assay for measuring daytime and nighttime chicken pineal and serum samples. An in vitro incubation of chicken pineal glands with a lighting cycle of 12-hr light: 12-hr dark showed that the diurnal rhythm of melatonin secretion into the cultured medium was maintained. The direct assay method described in this report for measuring chicken melatonin using 2-[125I]iodomelatonin as radiolabeled ligand coupled with the in vitro cultured chicken pineal gland clearly offers great potential for studying the chicken pineal circadian oscillator and its underlying mechanism.en_US
dc.languageengen_US
dc.publisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/en_US
dc.relation.ispartofProceedings of the Society for Experimental Biology and Medicineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshArylamine N-Acetyltransferase - Analysisen_US
dc.subject.meshChickensen_US
dc.subject.meshCircadian Rhythmen_US
dc.subject.meshMelatonin - Analysis - Secretionen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshPineal Gland - Secretionen_US
dc.subject.meshRadioimmunoassayen_US
dc.titleCircadian rhythms of melatonin release from chicken pineal in vitro: Modified melatonin radioimmunoassayen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1946475-
dc.identifier.scopuseid_2-s2.0-0026005565en_US
dc.identifier.volume198en_US
dc.identifier.issue3en_US
dc.identifier.spage826en_US
dc.identifier.epage832en_US
dc.identifier.isiWOS:A1991GR23700009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US

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