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Article: Flow cytometric measurement of cell cycle kinetics in rat Walker-256 carcinoma following in vivo and in vitro pulse labelling with bromodeoxyuridine

TitleFlow cytometric measurement of cell cycle kinetics in rat Walker-256 carcinoma following in vivo and in vitro pulse labelling with bromodeoxyuridine
Authors
Issue Date1991
Citation
Cytometry, 1991, v. 12 n. 1, p. 33-41 How to Cite?
AbstractFlow cytometric measurements of total DNA content, cell cycle distribution, and bromodeoxyuridine (BrdUrd) uptake were made in rat Walker-256 carcinoma cells. After both in vivo and in vitro pulse labelling with BrdUrd, Walker-256 tumor cells were stained with propidium iodide (PI) to estimate the total DNA content and a monoclonal antibody against BrdUrd to estimate the relative amount of cells in S phase. BrdUrd-labelled single cell suspensions were harvested at different time intervals to determine the movement of these cells within the cell cycle. To increase BrdUrd uptake, fluorodeoxyuridine (FDU), a thymidine antagonist, was also applied in vivo and in vitro. The results indicated exponential growth characteristics for this tumor between days 5 and 8 after implantation. Tumor doubling times, derived from changes in tumor volume in vivo and from the increase in cell number in vitro were similar. The mean time for DNA synthesis was estimated from the relative movement of BrdUrd-labelled cells towards G2. The percent of cells labelled with BrdUrd and the DNA synthesis time were similar regardless of the mode of BrdUrd administration. This study demonstrates that BrdUrd labelling of rat Walker-256 carcinoma cells in vitro yields kinetic estimates of tumor proliferation during exponential growth similar to those with the administration of BrdUrd in the intact tumor-bearing rat.
Persistent Identifierhttp://hdl.handle.net/10722/178509
ISSN
2004 Impact Factor: 2.698
2007 SCImago Journal Rankings: 0.190
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFogt, Fen_US
dc.contributor.authorWan, Jen_US
dc.contributor.authorO'hara, Cen_US
dc.contributor.authorBistrian, BRen_US
dc.contributor.authorBlackburn, GLen_US
dc.contributor.authorIstfan, NWen_US
dc.date.accessioned2012-12-19T09:48:07Z-
dc.date.available2012-12-19T09:48:07Z-
dc.date.issued1991en_US
dc.identifier.citationCytometry, 1991, v. 12 n. 1, p. 33-41en_US
dc.identifier.issn0196-4763en_US
dc.identifier.urihttp://hdl.handle.net/10722/178509-
dc.description.abstractFlow cytometric measurements of total DNA content, cell cycle distribution, and bromodeoxyuridine (BrdUrd) uptake were made in rat Walker-256 carcinoma cells. After both in vivo and in vitro pulse labelling with BrdUrd, Walker-256 tumor cells were stained with propidium iodide (PI) to estimate the total DNA content and a monoclonal antibody against BrdUrd to estimate the relative amount of cells in S phase. BrdUrd-labelled single cell suspensions were harvested at different time intervals to determine the movement of these cells within the cell cycle. To increase BrdUrd uptake, fluorodeoxyuridine (FDU), a thymidine antagonist, was also applied in vivo and in vitro. The results indicated exponential growth characteristics for this tumor between days 5 and 8 after implantation. Tumor doubling times, derived from changes in tumor volume in vivo and from the increase in cell number in vitro were similar. The mean time for DNA synthesis was estimated from the relative movement of BrdUrd-labelled cells towards G2. The percent of cells labelled with BrdUrd and the DNA synthesis time were similar regardless of the mode of BrdUrd administration. This study demonstrates that BrdUrd labelling of rat Walker-256 carcinoma cells in vitro yields kinetic estimates of tumor proliferation during exponential growth similar to those with the administration of BrdUrd in the intact tumor-bearing rat.en_US
dc.languageengen_US
dc.relation.ispartofCytometryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBromodeoxyuridine - Diagnostic Use - Metabolism - Pharmacokineticsen_US
dc.subject.meshCarcinoma 256, Walker - Chemistry - Metabolism - Pathologyen_US
dc.subject.meshCell Cycle - Physiologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Transformation, Neoplastic - Metabolism - Pathologyen_US
dc.subject.meshDna - Analysis - Metabolismen_US
dc.subject.meshFlow Cytometry - Methodsen_US
dc.subject.meshFloxuridine - Diagnostic Useen_US
dc.subject.meshG1 Phase - Physiologyen_US
dc.subject.meshG2 Phase - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMathematicsen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshPropidium - Diagnostic Useen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshS Phase - Physiologyen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTumor Cells, Cultured - Chemistry - Metabolism - Pathologyen_US
dc.titleFlow cytometric measurement of cell cycle kinetics in rat Walker-256 carcinoma following in vivo and in vitro pulse labelling with bromodeoxyuridineen_US
dc.typeArticleen_US
dc.identifier.emailWan, J: jmfwan@hku.hken_US
dc.identifier.authorityWan, J=rp00798en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/cyto.990120106en_US
dc.identifier.pmid1825629-
dc.identifier.scopuseid_2-s2.0-0025961693en_US
dc.identifier.volume12en_US
dc.identifier.issue1en_US
dc.identifier.spage33en_US
dc.identifier.epage41en_US
dc.identifier.isiWOS:A1991EN24900005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFogt, F=7006446193en_US
dc.identifier.scopusauthoridWan, J=8930305000en_US
dc.identifier.scopusauthoridO'Hara, C=55198700400en_US
dc.identifier.scopusauthoridBistrian, BR=35463916700en_US
dc.identifier.scopusauthoridBlackburn, GL=7201722807en_US
dc.identifier.scopusauthoridIstfan, NW=7003819779en_US

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