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Article: Elevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs

TitleElevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs
Authors
Issue Date1991
PublisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/
Citation
Proceedings Of The Society For Experimental Biology And Medicine, 1991, v. 196 n. 4, p. 385-389 How to Cite?
AbstractThe objective of this study is to examine and characterize epidermal growth factor receptor (EGF-R) binding in inhaled plutonium-induced canine lung-tumor tissue and to compare it with that in normal canine lung tissue. Crude membrane preparations from normal and lung-tumor tissue from beagle dogs were examined in a radioreceptor assay, using 125I-labeled epidermal growth factor (EGF) as a ligand. Specific EGF receptor binding was determined in the presence of excess unlabeled EGF. We have examined EGF receptor binding in eight lung-tumor samples obtained from six dogs. Epidermal growth factor receptor binding was significantly greater in lung-tumor samples (31.38%) compared with that in normal lung tissue (3.76%). Scatchard plot analysis from the displacement assay revealed that there was no statistical difference in the binding affinity but significantly higher concentration of EGF-R sites in the lung-tumor tissue (619 fmol/mg) than in normal lung tissue (53 fmol/mg). The increase in EGF-R number in plutonium-induced dog lung tumors does not seem to correlate with increase in the initial lung burden exposure to plutonium. Our results demonstrate that there is a significant increase in EGF-R binding in inhaled plutonium-induced dog lung tumors.
Persistent Identifierhttp://hdl.handle.net/10722/178507
ISSN
2002 Impact Factor: 2.714
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, FCen_US
dc.contributor.authorBohn, LRen_US
dc.contributor.authorDagle, GEen_US
dc.date.accessioned2012-12-19T09:48:06Z-
dc.date.available2012-12-19T09:48:06Z-
dc.date.issued1991en_US
dc.identifier.citationProceedings Of The Society For Experimental Biology And Medicine, 1991, v. 196 n. 4, p. 385-389en_US
dc.identifier.issn0037-9727en_US
dc.identifier.urihttp://hdl.handle.net/10722/178507-
dc.description.abstractThe objective of this study is to examine and characterize epidermal growth factor receptor (EGF-R) binding in inhaled plutonium-induced canine lung-tumor tissue and to compare it with that in normal canine lung tissue. Crude membrane preparations from normal and lung-tumor tissue from beagle dogs were examined in a radioreceptor assay, using 125I-labeled epidermal growth factor (EGF) as a ligand. Specific EGF receptor binding was determined in the presence of excess unlabeled EGF. We have examined EGF receptor binding in eight lung-tumor samples obtained from six dogs. Epidermal growth factor receptor binding was significantly greater in lung-tumor samples (31.38%) compared with that in normal lung tissue (3.76%). Scatchard plot analysis from the displacement assay revealed that there was no statistical difference in the binding affinity but significantly higher concentration of EGF-R sites in the lung-tumor tissue (619 fmol/mg) than in normal lung tissue (53 fmol/mg). The increase in EGF-R number in plutonium-induced dog lung tumors does not seem to correlate with increase in the initial lung burden exposure to plutonium. Our results demonstrate that there is a significant increase in EGF-R binding in inhaled plutonium-induced dog lung tumors.en_US
dc.languageengen_US
dc.publisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/en_US
dc.relation.ispartofProceedings of the Society for Experimental Biology and Medicineen_US
dc.subject.meshAdenocarcinoma, Papillary - Metabolismen_US
dc.subject.meshAdministration, Inhalationen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshCarcinoma, Bronchogenic - Metabolismen_US
dc.subject.meshDogsen_US
dc.subject.meshEpidermal Growth Factor - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshLung Neoplasms - Etiology - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Metabolismen_US
dc.subject.meshNeoplasms, Radiation-Induceden_US
dc.subject.meshPlutoniumen_US
dc.subject.meshRadioligand Assayen_US
dc.subject.meshReceptor, Epidermal Growth Factor - Metabolismen_US
dc.titleElevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2008439-
dc.identifier.scopuseid_2-s2.0-0025911581en_US
dc.identifier.volume196en_US
dc.identifier.issue4en_US
dc.identifier.spage385en_US
dc.identifier.epage389en_US
dc.identifier.isiWOS:A1991FE55900004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US
dc.identifier.scopusauthoridBohn, LR=7003466457en_US
dc.identifier.scopusauthoridDagle, GE=7004357391en_US

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