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Article: Laticifer-specific gene expression in Hevea brasiliensis (rubber tree)

TitleLaticifer-specific gene expression in Hevea brasiliensis (rubber tree)
Authors
KeywordsCell Specificity
Ethylene
Latex
Plant Defense Genes
Rubber Biosynthesis
Issue Date1990
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1990, v. 87 n. 5, p. 1787-1790 How to Cite?
AbstractNatural rubber, cis-1,4-polyisoprene, is obtained from a colloidal fluid called latex, which represents the cytoplasmic content of the laticifers of the rubber tree (Hevea brasiliensis). We have developed a method of extracting translatable mRNA from freshly tapped latex. Analysis of in vitro translation products of latex mRNA showed that the encoded polypeptides are very different from those of leaf mRNA and these differences are visible in the protein profiles of latex and leaf as well. Northern blot analysis demonstrated that laticifer RNA is 20- to 100-fold enriched in transcripts encoding enzymes involved in rubber biosynthesis. Plant defense genes encoding chitinases, pathogenesis-related protein, phenylalanine ammonia-lyase, chalcone synthase, chalcone isomerase, cinnamyl alcohol dehydrogenase, and 5-enolpyruvylshikimate-3-phosphate synthase show a 10- to 50-fold higher expression in laticifers than in leaves, indicating the probable response of rubber trees to tapping and ethylene treatment. Photosynthetic genes encoding ribulose-bisphosphate carboxylase small subunit and chlorophyll a/b-binding protein are not expressed at a detectable level in laticifers. In contrast, genes encoding two hydrolytic enzymes, cellulase and polygalacturonase, are more highly expressed in laticifers than in leaves. Transcripts for the cytoplasmic form of glutamine synthase are preferentially expressed in laticifers, whereas those for the chloroplastic form of the same enzyme are present mainly in leaves. Control experiments demonstrated that β-ATPase, actin, and ubiquitin are equally expressed in laticifers and leaves. Therefore, the differences in specific transcript abundance between laticifers and leaves are due to differential expression of the genes for these transcripts in the laticifers.
Persistent Identifierhttp://hdl.handle.net/10722/178489
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883

 

DC FieldValueLanguage
dc.contributor.authorKush, Aen_US
dc.contributor.authorGoyvaerts, Een_US
dc.contributor.authorChye, MLen_US
dc.contributor.authorChua, NHen_US
dc.date.accessioned2012-12-19T09:48:00Z-
dc.date.available2012-12-19T09:48:00Z-
dc.date.issued1990en_US
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1990, v. 87 n. 5, p. 1787-1790en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/178489-
dc.description.abstractNatural rubber, cis-1,4-polyisoprene, is obtained from a colloidal fluid called latex, which represents the cytoplasmic content of the laticifers of the rubber tree (Hevea brasiliensis). We have developed a method of extracting translatable mRNA from freshly tapped latex. Analysis of in vitro translation products of latex mRNA showed that the encoded polypeptides are very different from those of leaf mRNA and these differences are visible in the protein profiles of latex and leaf as well. Northern blot analysis demonstrated that laticifer RNA is 20- to 100-fold enriched in transcripts encoding enzymes involved in rubber biosynthesis. Plant defense genes encoding chitinases, pathogenesis-related protein, phenylalanine ammonia-lyase, chalcone synthase, chalcone isomerase, cinnamyl alcohol dehydrogenase, and 5-enolpyruvylshikimate-3-phosphate synthase show a 10- to 50-fold higher expression in laticifers than in leaves, indicating the probable response of rubber trees to tapping and ethylene treatment. Photosynthetic genes encoding ribulose-bisphosphate carboxylase small subunit and chlorophyll a/b-binding protein are not expressed at a detectable level in laticifers. In contrast, genes encoding two hydrolytic enzymes, cellulase and polygalacturonase, are more highly expressed in laticifers than in leaves. Transcripts for the cytoplasmic form of glutamine synthase are preferentially expressed in laticifers, whereas those for the chloroplastic form of the same enzyme are present mainly in leaves. Control experiments demonstrated that β-ATPase, actin, and ubiquitin are equally expressed in laticifers and leaves. Therefore, the differences in specific transcript abundance between laticifers and leaves are due to differential expression of the genes for these transcripts in the laticifers.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subjectCell Specificityen_US
dc.subjectEthyleneen_US
dc.subjectLatexen_US
dc.subjectPlant Defense Genesen_US
dc.subjectRubber Biosynthesisen_US
dc.titleLaticifer-specific gene expression in Hevea brasiliensis (rubber tree)en_US
dc.typeArticleen_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0025261346en_US
dc.identifier.volume87en_US
dc.identifier.issue5en_US
dc.identifier.spage1787en_US
dc.identifier.epage1790en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKush, A=6603781579en_US
dc.identifier.scopusauthoridGoyvaerts, E=6602273176en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US
dc.identifier.scopusauthoridChua, NH=7103077967en_US

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