File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1093/nar/18.18.5393
- Scopus: eid_2-s2.0-0025011104
- PMID: 2120676
- WOS: WOS:A1990EA88200009
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1
Title | ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1 |
---|---|
Authors | |
Issue Date | 1990 |
Publisher | Oxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/ |
Citation | Nucleic Acids Research, 1990, v. 18 n. 18, p. 5393-5399 How to Cite? |
Abstract | The UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the activator core sequence, which is bound by the RAP1 protein, and three copies of the pentamer sequence 5' CTTCC 3'. Upstream of the activator core sequence is a region (Yfp), identified as the site of a strong DNA-protein interaction. The Yfp region contains the consensus binding site for the factor ABF1. We have purified the Y protein, which binds to the Yfp region, to homogeneity. The Y protein migrates as a doublet on SDS-polyacrylamide gel electrophoresis with an apparent molecular weight of 125 KDa. These properties are similar to those of ABF1. ABF1 synthesised in vitro bound strongly to the Yfp region and formed a gel retardation complex of identical mobility to the complex formed by the Y protein. UAS1 of the pyruvate kinase gene (PYK1) promoter contains a RAP1 binding site and single copy of the CTTCC sequence. We have now identified an ABF1 binding site close to the RAP1 binding site and CTTCC sequence in the PYK1 promoter. This site is strongly bound by ABF1 in vitro. The organisation of the PGK and PYK1 UASs is thus similar to each other and to the transcriptional silencer HMR(E) which also contains these sequences. |
Persistent Identifier | http://hdl.handle.net/10722/178482 |
ISSN | 2021 Impact Factor: 19.160 2020 SCImago Journal Rankings: 9.008 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chambers, A | en_US |
dc.contributor.author | Stanway, C | en_US |
dc.contributor.author | Tsang, JSH | en_US |
dc.contributor.author | Henry, Y | en_US |
dc.contributor.author | Kingsman, AJ | en_US |
dc.contributor.author | Kingsman, SM | en_US |
dc.date.accessioned | 2012-12-19T09:47:56Z | - |
dc.date.available | 2012-12-19T09:47:56Z | - |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Nucleic Acids Research, 1990, v. 18 n. 18, p. 5393-5399 | en_US |
dc.identifier.issn | 0305-1048 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178482 | - |
dc.description.abstract | The UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the activator core sequence, which is bound by the RAP1 protein, and three copies of the pentamer sequence 5' CTTCC 3'. Upstream of the activator core sequence is a region (Yfp), identified as the site of a strong DNA-protein interaction. The Yfp region contains the consensus binding site for the factor ABF1. We have purified the Y protein, which binds to the Yfp region, to homogeneity. The Y protein migrates as a doublet on SDS-polyacrylamide gel electrophoresis with an apparent molecular weight of 125 KDa. These properties are similar to those of ABF1. ABF1 synthesised in vitro bound strongly to the Yfp region and formed a gel retardation complex of identical mobility to the complex formed by the Y protein. UAS1 of the pyruvate kinase gene (PYK1) promoter contains a RAP1 binding site and single copy of the CTTCC sequence. We have now identified an ABF1 binding site close to the RAP1 binding site and CTTCC sequence in the PYK1 promoter. This site is strongly bound by ABF1 in vitro. The organisation of the PGK and PYK1 UASs is thus similar to each other and to the transcriptional silencer HMR(E) which also contains these sequences. | en_US |
dc.language | eng | en_US |
dc.publisher | Oxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/ | en_US |
dc.relation.ispartof | Nucleic Acids Research | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Binding Sites | en_US |
dc.subject.mesh | Binding, Competitive | en_US |
dc.subject.mesh | Carrier Proteins - Isolation & Purification - Metabolism | en_US |
dc.subject.mesh | Dna - Genetics - Metabolism | en_US |
dc.subject.mesh | Gtp-Binding Proteins - Metabolism | en_US |
dc.subject.mesh | Gene Expression Regulation, Fungal | en_US |
dc.subject.mesh | Genes, Fungal | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Phosphoglycerate Kinase - Genetics - Metabolism | en_US |
dc.subject.mesh | Promoter Regions, Genetic | en_US |
dc.subject.mesh | Pyruvate Kinase - Genetics - Metabolism | en_US |
dc.subject.mesh | Regulatory Sequences, Nucleic Acid | en_US |
dc.subject.mesh | Saccharomyces Cerevisiae - Genetics | en_US |
dc.subject.mesh | Transcription Factors - Metabolism | en_US |
dc.subject.mesh | Rap Gtp-Binding Proteins | en_US |
dc.title | ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1 | en_US |
dc.type | Article | en_US |
dc.identifier.email | Tsang, JSH: jshtsang@hku.hk | en_US |
dc.identifier.authority | Tsang, JSH=rp00792 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1093/nar/18.18.5393 | - |
dc.identifier.pmid | 2120676 | - |
dc.identifier.pmcid | PMC332215 | - |
dc.identifier.scopus | eid_2-s2.0-0025011104 | en_US |
dc.identifier.volume | 18 | en_US |
dc.identifier.issue | 18 | en_US |
dc.identifier.spage | 5393 | en_US |
dc.identifier.epage | 5399 | en_US |
dc.identifier.isi | WOS:A1990EA88200009 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Chambers, A=35563879400 | en_US |
dc.identifier.scopusauthorid | Stanway, C=6603949543 | en_US |
dc.identifier.scopusauthorid | Tsang, JSH=7102483508 | en_US |
dc.identifier.scopusauthorid | Henry, Y=26642929300 | en_US |
dc.identifier.scopusauthorid | Kingsman, AJ=7006672393 | en_US |
dc.identifier.scopusauthorid | Kingsman, SM=7004417560 | en_US |
dc.identifier.issnl | 0305-1048 | - |