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Article: ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1

TitleARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1
Authors
Issue Date1990
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1990, v. 18 n. 18, p. 5393-5399 How to Cite?
AbstractThe UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the activator core sequence, which is bound by the RAP1 protein, and three copies of the pentamer sequence 5' CTTCC 3'. Upstream of the activator core sequence is a region (Yfp), identified as the site of a strong DNA-protein interaction. The Yfp region contains the consensus binding site for the factor ABF1. We have purified the Y protein, which binds to the Yfp region, to homogeneity. The Y protein migrates as a doublet on SDS-polyacrylamide gel electrophoresis with an apparent molecular weight of 125 KDa. These properties are similar to those of ABF1. ABF1 synthesised in vitro bound strongly to the Yfp region and formed a gel retardation complex of identical mobility to the complex formed by the Y protein. UAS1 of the pyruvate kinase gene (PYK1) promoter contains a RAP1 binding site and single copy of the CTTCC sequence. We have now identified an ABF1 binding site close to the RAP1 binding site and CTTCC sequence in the PYK1 promoter. This site is strongly bound by ABF1 in vitro. The organisation of the PGK and PYK1 UASs is thus similar to each other and to the transcriptional silencer HMR(E) which also contains these sequences.
Persistent Identifierhttp://hdl.handle.net/10722/178482
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChambers, Aen_US
dc.contributor.authorStanway, Cen_US
dc.contributor.authorTsang, JSHen_US
dc.contributor.authorHenry, Yen_US
dc.contributor.authorKingsman, AJen_US
dc.contributor.authorKingsman, SMen_US
dc.date.accessioned2012-12-19T09:47:56Z-
dc.date.available2012-12-19T09:47:56Z-
dc.date.issued1990en_US
dc.identifier.citationNucleic Acids Research, 1990, v. 18 n. 18, p. 5393-5399en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/178482-
dc.description.abstractThe UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the activator core sequence, which is bound by the RAP1 protein, and three copies of the pentamer sequence 5' CTTCC 3'. Upstream of the activator core sequence is a region (Yfp), identified as the site of a strong DNA-protein interaction. The Yfp region contains the consensus binding site for the factor ABF1. We have purified the Y protein, which binds to the Yfp region, to homogeneity. The Y protein migrates as a doublet on SDS-polyacrylamide gel electrophoresis with an apparent molecular weight of 125 KDa. These properties are similar to those of ABF1. ABF1 synthesised in vitro bound strongly to the Yfp region and formed a gel retardation complex of identical mobility to the complex formed by the Y protein. UAS1 of the pyruvate kinase gene (PYK1) promoter contains a RAP1 binding site and single copy of the CTTCC sequence. We have now identified an ABF1 binding site close to the RAP1 binding site and CTTCC sequence in the PYK1 promoter. This site is strongly bound by ABF1 in vitro. The organisation of the PGK and PYK1 UASs is thus similar to each other and to the transcriptional silencer HMR(E) which also contains these sequences.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBinding, Competitiveen_US
dc.subject.meshCarrier Proteins - Isolation & Purification - Metabolismen_US
dc.subject.meshDna - Genetics - Metabolismen_US
dc.subject.meshGtp-Binding Proteins - Metabolismen_US
dc.subject.meshGene Expression Regulation, Fungalen_US
dc.subject.meshGenes, Fungalen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPhosphoglycerate Kinase - Genetics - Metabolismen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshPyruvate Kinase - Genetics - Metabolismen_US
dc.subject.meshRegulatory Sequences, Nucleic Aciden_US
dc.subject.meshSaccharomyces Cerevisiae - Geneticsen_US
dc.subject.meshTranscription Factors - Metabolismen_US
dc.subject.meshRap Gtp-Binding Proteinsen_US
dc.titleARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1en_US
dc.typeArticleen_US
dc.identifier.emailTsang, JSH: jshtsang@hku.hken_US
dc.identifier.authorityTsang, JSH=rp00792en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/18.18.5393-
dc.identifier.pmid2120676-
dc.identifier.scopuseid_2-s2.0-0025011104en_US
dc.identifier.volume18en_US
dc.identifier.issue18en_US
dc.identifier.spage5393en_US
dc.identifier.epage5399en_US
dc.identifier.isiWOS:A1990EA88200009-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChambers, A=35563879400en_US
dc.identifier.scopusauthoridStanway, C=6603949543en_US
dc.identifier.scopusauthoridTsang, JSH=7102483508en_US
dc.identifier.scopusauthoridHenry, Y=26642929300en_US
dc.identifier.scopusauthoridKingsman, AJ=7006672393en_US
dc.identifier.scopusauthoridKingsman, SM=7004417560en_US

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