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Article: Effect of phenolic monomers on the production of laccases by the edible mushroom Pleurotus sajor-caju, and partial characterization of a major laccase component

TitleEffect of phenolic monomers on the production of laccases by the edible mushroom Pleurotus sajor-caju, and partial characterization of a major laccase component
Authors
KeywordsPhenols
Toxicity
Issue Date2002
PublisherMycological Society of America. The Journal's web site is located at http://www.msafungi.org
Citation
Mycologia, 2002, v. 93 n. 3, p. 413-421 How to Cite?
AbstractThe effects of several phenolic monomers on the growth and laccase production by the edible mushroom Pleurotus sajor-caju have been compared. Of the phenols tested, 4-hydroxybenzaldehyde and vanillin were most inhibitory to fungal growth, and cultures supplemented with these two compounds also exhibited the largest increases in laccase specific activity compared with unsupplemented controls. Up to five laccase isoforms were detected in culture fluids when P. sajor-caju was grown in submerged culture in the presence of several phenolic monomers. A major laccase component (laccase IV) present in P. sajor-caju culture supernatants was purified 152-fold with an overall recovery of 4.9%. Laccase IV was shown to be homogeneous by SDS-PAGE and isoelectric focusing, and had a molecular weight of 55 kD and an isoelectric point of 3.6. It displayed a maximum reaction velocity at pH 2.1 and 45 C, and the activation energy of the enzyme reaction between 25 and 45 C as determined by an Arrhenius plot was 12.4 kJ mol-1. The Km of laccase IV, as determined using ABTS as the substrate, was 0.092 mM. Laccase IV was glycosylated with mannose as the major sugar component together with galactose, glucosamine and fucose. Comparison of up to thirteen N-terminal amino acids of laccase IV with those of other fungal laccases revealed the highest similarity (85%) with a laccase from Pleurotus ostreatus. Considerable similarity (46-50%) was also observed with laccases from Coriolus versicolor, Pycnoporus cinnabarinus, Phlebia radiata, Coriolus hirsutus and Ceriporiopsis subvermispora but not Agaricus bisporus.
Persistent Identifierhttp://hdl.handle.net/10722/178393
ISSN
2015 Impact Factor: 2.638
2015 SCImago Journal Rankings: 1.145
References

 

DC FieldValueLanguage
dc.contributor.authorLo, SCen_US
dc.contributor.authorHo, YSen_US
dc.contributor.authorBuswell, JAen_US
dc.date.accessioned2012-12-19T09:47:26Z-
dc.date.available2012-12-19T09:47:26Z-
dc.date.issued2002en_US
dc.identifier.citationMycologia, 2002, v. 93 n. 3, p. 413-421en_US
dc.identifier.issn0027-5514en_US
dc.identifier.urihttp://hdl.handle.net/10722/178393-
dc.description.abstractThe effects of several phenolic monomers on the growth and laccase production by the edible mushroom Pleurotus sajor-caju have been compared. Of the phenols tested, 4-hydroxybenzaldehyde and vanillin were most inhibitory to fungal growth, and cultures supplemented with these two compounds also exhibited the largest increases in laccase specific activity compared with unsupplemented controls. Up to five laccase isoforms were detected in culture fluids when P. sajor-caju was grown in submerged culture in the presence of several phenolic monomers. A major laccase component (laccase IV) present in P. sajor-caju culture supernatants was purified 152-fold with an overall recovery of 4.9%. Laccase IV was shown to be homogeneous by SDS-PAGE and isoelectric focusing, and had a molecular weight of 55 kD and an isoelectric point of 3.6. It displayed a maximum reaction velocity at pH 2.1 and 45 C, and the activation energy of the enzyme reaction between 25 and 45 C as determined by an Arrhenius plot was 12.4 kJ mol-1. The Km of laccase IV, as determined using ABTS as the substrate, was 0.092 mM. Laccase IV was glycosylated with mannose as the major sugar component together with galactose, glucosamine and fucose. Comparison of up to thirteen N-terminal amino acids of laccase IV with those of other fungal laccases revealed the highest similarity (85%) with a laccase from Pleurotus ostreatus. Considerable similarity (46-50%) was also observed with laccases from Coriolus versicolor, Pycnoporus cinnabarinus, Phlebia radiata, Coriolus hirsutus and Ceriporiopsis subvermispora but not Agaricus bisporus.en_US
dc.languageengen_US
dc.publisherMycological Society of America. The Journal's web site is located at http://www.msafungi.orgen_US
dc.relation.ispartofMycologiaen_US
dc.subjectPhenolsen_US
dc.subjectToxicityen_US
dc.titleEffect of phenolic monomers on the production of laccases by the edible mushroom Pleurotus sajor-caju, and partial characterization of a major laccase componenten_US
dc.typeArticleen_US
dc.identifier.emailLo, SC: clivelo@hkucc.hku.hken_US
dc.identifier.authorityLo, SC=rp00751en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0013043137en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0013043137&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume93en_US
dc.identifier.issue3en_US
dc.identifier.spage413en_US
dc.identifier.epage421en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLo, SC=15737175700en_US
dc.identifier.scopusauthoridHo, YS=8679200700en_US
dc.identifier.scopusauthoridBuswell, JA=7006560852en_US

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