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Article: Cloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruit

TitleCloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruit
Authors
Keywords1-Amninocyclopropane-1-Carboxylate Synthase
Cdna
Ethylene Synthesis
Fruit Ripening
Gene Expression
Malus
Issue Date1991
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 1991, v. 185 n. 1, p. 38-45 How to Cite?
Abstract1-Aminocyclopropane-1-carboxylate (ACC) synthase (EC 4.4.1.14) purified from apple (Malus sylvestris Mill.) fruit was subjected to trypsin digestion. Following separation by reversed-phase high-pressure liquid chromatography, ten tryptic peptides were sequenced. Based on the sequences of three tryptic peptides, three sets of mixed oligonucleotide probes were synthesized and used to screen a plasmid cDNA library prepared from poly(A) + RNA of ripe apple fruit. A 1.5-kb (kilobase) cDNA clone which hybridized to all three probes were isolated. The clone contained an open reading frame of 1214 base pairs (bp) encoding a sequence of 404 amino acids. While the polyadenine tail at the 3′-end was intact, it lacked a portion of sequence at the 5′-end. Using the RNA-based polymerase chain reaction, an additional sequence of 148 bp was obtained at the 5′-end. Thus, 1362 bp were sequenced and they encode 454 amino acids. The deduced amino-acid sequence contained peptide sequences corresponding to all ten tryptic fragments, confirming the identity of the cDNA clone. Comparison of the deduced amino-acid sequence between ACC synthase from apple fruit and those from tomato (Lycopersicon esculentum Mill.) and winter squash (Cucurbita maxima Duch.) fruits demonstrated the presence of seven highly conserved regions, including the previously identified region for the active site. The size of the translation product of ACC-synthase mRNA was similar to that of the mature protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), indicating that apple ACC-synthase undergoes only minor, if any, post-translational proteolytic processing. Analysis of ACC-synthase mRNA by in-vitro translation-immunoprecipitation, and by Northern blotting indicates that the ACC-synthase mRNA was undetectable in unripe fruit, but was accumulated massively during the ripening proccess. These data demonstrate that the expression of the ACC-synthase gene is developmentally regulated. © 1991 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/178384
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470

 

DC FieldValueLanguage
dc.contributor.authorDong, JGen_US
dc.contributor.authorKim, WTen_US
dc.contributor.authorYip, WKen_US
dc.contributor.authorThompson, GAen_US
dc.contributor.authorLi, Len_US
dc.contributor.authorBennett, ABen_US
dc.contributor.authorYang, SFen_US
dc.date.accessioned2012-12-19T09:47:23Z-
dc.date.available2012-12-19T09:47:23Z-
dc.date.issued1991en_US
dc.identifier.citationPlanta, 1991, v. 185 n. 1, p. 38-45en_US
dc.identifier.issn0032-0935en_US
dc.identifier.urihttp://hdl.handle.net/10722/178384-
dc.description.abstract1-Aminocyclopropane-1-carboxylate (ACC) synthase (EC 4.4.1.14) purified from apple (Malus sylvestris Mill.) fruit was subjected to trypsin digestion. Following separation by reversed-phase high-pressure liquid chromatography, ten tryptic peptides were sequenced. Based on the sequences of three tryptic peptides, three sets of mixed oligonucleotide probes were synthesized and used to screen a plasmid cDNA library prepared from poly(A) + RNA of ripe apple fruit. A 1.5-kb (kilobase) cDNA clone which hybridized to all three probes were isolated. The clone contained an open reading frame of 1214 base pairs (bp) encoding a sequence of 404 amino acids. While the polyadenine tail at the 3′-end was intact, it lacked a portion of sequence at the 5′-end. Using the RNA-based polymerase chain reaction, an additional sequence of 148 bp was obtained at the 5′-end. Thus, 1362 bp were sequenced and they encode 454 amino acids. The deduced amino-acid sequence contained peptide sequences corresponding to all ten tryptic fragments, confirming the identity of the cDNA clone. Comparison of the deduced amino-acid sequence between ACC synthase from apple fruit and those from tomato (Lycopersicon esculentum Mill.) and winter squash (Cucurbita maxima Duch.) fruits demonstrated the presence of seven highly conserved regions, including the previously identified region for the active site. The size of the translation product of ACC-synthase mRNA was similar to that of the mature protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), indicating that apple ACC-synthase undergoes only minor, if any, post-translational proteolytic processing. Analysis of ACC-synthase mRNA by in-vitro translation-immunoprecipitation, and by Northern blotting indicates that the ACC-synthase mRNA was undetectable in unripe fruit, but was accumulated massively during the ripening proccess. These data demonstrate that the expression of the ACC-synthase gene is developmentally regulated. © 1991 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_US
dc.relation.ispartofPlantaen_US
dc.subject1-Amninocyclopropane-1-Carboxylate Synthaseen_US
dc.subjectCdnaen_US
dc.subjectEthylene Synthesisen_US
dc.subjectFruit Ripeningen_US
dc.subjectGene Expressionen_US
dc.subjectMalusen_US
dc.titleCloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruiten_US
dc.typeArticleen_US
dc.identifier.emailYip, WK: wkyip@hkucc.hku.hken_US
dc.identifier.authorityYip, WK=rp00833en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00194512en_US
dc.identifier.scopuseid_2-s2.0-0003079379en_US
dc.identifier.volume185en_US
dc.identifier.issue1en_US
dc.identifier.spage38en_US
dc.identifier.epage45en_US
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridDong, JG=55477984500en_US
dc.identifier.scopusauthoridKim, WT=7405812790en_US
dc.identifier.scopusauthoridYip, WK=7102784428en_US
dc.identifier.scopusauthoridThompson, GA=7403077227en_US
dc.identifier.scopusauthoridLi, L=7501454876en_US
dc.identifier.scopusauthoridBennett, AB=7402332568en_US
dc.identifier.scopusauthoridYang, SF=7406946528en_US

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