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Article: Induction of 1-aminocyclopropane-1-carboxylate synthase mRNA by auxin in mung bean hypocotyls and cultured apple shoots

TitleInduction of 1-aminocyclopropane-1-carboxylate synthase mRNA by auxin in mung bean hypocotyls and cultured apple shoots
Authors
Issue Date1992
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 1992, v. 98 n. 2, p. 465-471 How to Cite?
AbstractAuxin is known to promote ethylene production in vegetative tissues by increasing the activity of 1-aminocyclopropane-1-carboxylate (ACC) synthase; therefore, we have studied the effect of auxins on ACC synthase mRNA expression. Total RNA was isolated from auxin-incubated cultured apple (Malus sylvestris Mill.) shoots or mung bean (Vigna radiata L.) hypocotyls. These RNAs and a set of oligonucleotide primers corresponding to two conserved amino acid sequences (SNPLGTT and MSSFGLV) found in ACC syntheses isolated from other species were used for polymerase chain reaction-based amplification of DMA fragments encoding the ACC synthase-active site domain. We obtained and sequenced a 290-base pair cDNA fragment (pAA1) from cultured apple shoots and a 328-base pair cDNA clone (pMBA1) from mung bean hypocotyls. Comparisons of their deduced amino acid sequences with those of previously characterized ACC synthase cDNAs indicate that both fragments are, indeed, closely related to ACC synthase cDNA. Northern blot analyses further showed that the expression of these transcripts is regulated by auxin treatment. These data indicate that auxin induces ethylene production transcriptionally by increasing the ACC synthase transcripts. The pAA1 shares 46% amino acid sequence homology with ripening-regulated apple fruit ACC synthase, indicating that ripening-regulated and auxin-regulated ACC synthases are encoded by different genes. In mung bean hypocotyls, aminooxyacetic acid, a potent inhibitor of ACC synthase activity, promoted the expression of auxin-induced ACC synthase mRNA, but cycloheximide inhibited this induction.
Persistent Identifierhttp://hdl.handle.net/10722/178364
ISSN
2015 Impact Factor: 6.28
2015 SCImago Journal Rankings: 3.642

 

DC FieldValueLanguage
dc.contributor.authorKim, WTen_US
dc.contributor.authorSilverstone, Aen_US
dc.contributor.authorYip, WKen_US
dc.contributor.authorDong, JGen_US
dc.contributor.authorYang, SFen_US
dc.date.accessioned2012-12-19T09:47:17Z-
dc.date.available2012-12-19T09:47:17Z-
dc.date.issued1992en_US
dc.identifier.citationPlant Physiology, 1992, v. 98 n. 2, p. 465-471en_US
dc.identifier.issn0032-0889en_US
dc.identifier.urihttp://hdl.handle.net/10722/178364-
dc.description.abstractAuxin is known to promote ethylene production in vegetative tissues by increasing the activity of 1-aminocyclopropane-1-carboxylate (ACC) synthase; therefore, we have studied the effect of auxins on ACC synthase mRNA expression. Total RNA was isolated from auxin-incubated cultured apple (Malus sylvestris Mill.) shoots or mung bean (Vigna radiata L.) hypocotyls. These RNAs and a set of oligonucleotide primers corresponding to two conserved amino acid sequences (SNPLGTT and MSSFGLV) found in ACC syntheses isolated from other species were used for polymerase chain reaction-based amplification of DMA fragments encoding the ACC synthase-active site domain. We obtained and sequenced a 290-base pair cDNA fragment (pAA1) from cultured apple shoots and a 328-base pair cDNA clone (pMBA1) from mung bean hypocotyls. Comparisons of their deduced amino acid sequences with those of previously characterized ACC synthase cDNAs indicate that both fragments are, indeed, closely related to ACC synthase cDNA. Northern blot analyses further showed that the expression of these transcripts is regulated by auxin treatment. These data indicate that auxin induces ethylene production transcriptionally by increasing the ACC synthase transcripts. The pAA1 shares 46% amino acid sequence homology with ripening-regulated apple fruit ACC synthase, indicating that ripening-regulated and auxin-regulated ACC synthases are encoded by different genes. In mung bean hypocotyls, aminooxyacetic acid, a potent inhibitor of ACC synthase activity, promoted the expression of auxin-induced ACC synthase mRNA, but cycloheximide inhibited this induction.en_US
dc.languageengen_US
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.orgen_US
dc.relation.ispartofPlant Physiologyen_US
dc.titleInduction of 1-aminocyclopropane-1-carboxylate synthase mRNA by auxin in mung bean hypocotyls and cultured apple shootsen_US
dc.typeArticleen_US
dc.identifier.emailYip, WK: wkyip@hkucc.hku.hken_US
dc.identifier.authorityYip, WK=rp00833en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0000576561en_US
dc.identifier.volume98en_US
dc.identifier.issue2en_US
dc.identifier.spage465en_US
dc.identifier.epage471en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKim, WT=7405812790en_US
dc.identifier.scopusauthoridSilverstone, A=36860998900en_US
dc.identifier.scopusauthoridYip, WK=7102784428en_US
dc.identifier.scopusauthoridDong, JG=55477984500en_US
dc.identifier.scopusauthoridYang, SF=7406946528en_US

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