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Article: A comparative study on potential cytogenetic fingerprints for radiation LET in human lymphocytes

TitleA comparative study on potential cytogenetic fingerprints for radiation LET in human lymphocytes
Authors
Issue Date2000
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09553002.asp
Citation
International Journal Of Radiation Biology, 2000, v. 76 n. 12, p. 1589-1598 How to Cite?
AbstractPurpose: To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. Materials and methods: Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co γ-rays, 0.9 Gy 3H β-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by combined FISH with pan-telomere staining and specific whole-chromosome painting (1, 2 and 4). Total chromosome translocations and insertions were also analysed by multicolour whole-chromosome painting (chromosomes 1, 2 and 4 orange, chromosomes 3, 5 and 6 green). Results: Among the six proposed radiation cytogenetic fingerprints, the ratio of total simple translocations to insertions (I-ratio), showed the largest difference between low-LET 60Co γ-ray and high-LET neutron radiation. The ratios of complete exchanges to incomplete rejoinings [S(I)-ratio] and dicentrics to interstitial deletions (H-ratio), showed a similar significant difference between low- and high-LET radiation. The ratios of centric rings to interstitial deletion (G-ratio) showed a trend of LET-related difference, but the difference was not significant in this data set. The ratios of dicentrics to centric rings (F-ratio) and apparent complete exchanges to hidden complete exchanges [S(II)-ratio], showed no difference between low- and high-LET radiation. In the 1426 radiation-induced chromosome aberrations observed after 52 h culture, evidence for sister-chromatid fusion but not telomere addition was found. Conclusion: Pan-telomere staining plus specific whole chromosome painting allows simultaneous and objective detection of complete or incomplete chromosome exchanges and interstitial or terminal deletions in human peripheral lymphocytes. Of the six proposed cytogenetic ratios, the I-ratio is the most effective cytogenetic fingerprint for distinguishing low-LET from high-LET radiation in human metaphase human lymphocytes.
Persistent Identifierhttp://hdl.handle.net/10722/178265
ISSN
2015 Impact Factor: 1.779
2015 SCImago Journal Rankings: 0.734
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDeng, Wen_US
dc.contributor.authorMorrison, DPen_US
dc.contributor.authorGale, KLen_US
dc.contributor.authorLucas, JNen_US
dc.date.accessioned2012-12-19T09:44:53Z-
dc.date.available2012-12-19T09:44:53Z-
dc.date.issued2000en_US
dc.identifier.citationInternational Journal Of Radiation Biology, 2000, v. 76 n. 12, p. 1589-1598en_US
dc.identifier.issn0955-3002en_US
dc.identifier.urihttp://hdl.handle.net/10722/178265-
dc.description.abstractPurpose: To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. Materials and methods: Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co γ-rays, 0.9 Gy 3H β-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by combined FISH with pan-telomere staining and specific whole-chromosome painting (1, 2 and 4). Total chromosome translocations and insertions were also analysed by multicolour whole-chromosome painting (chromosomes 1, 2 and 4 orange, chromosomes 3, 5 and 6 green). Results: Among the six proposed radiation cytogenetic fingerprints, the ratio of total simple translocations to insertions (I-ratio), showed the largest difference between low-LET 60Co γ-ray and high-LET neutron radiation. The ratios of complete exchanges to incomplete rejoinings [S(I)-ratio] and dicentrics to interstitial deletions (H-ratio), showed a similar significant difference between low- and high-LET radiation. The ratios of centric rings to interstitial deletion (G-ratio) showed a trend of LET-related difference, but the difference was not significant in this data set. The ratios of dicentrics to centric rings (F-ratio) and apparent complete exchanges to hidden complete exchanges [S(II)-ratio], showed no difference between low- and high-LET radiation. In the 1426 radiation-induced chromosome aberrations observed after 52 h culture, evidence for sister-chromatid fusion but not telomere addition was found. Conclusion: Pan-telomere staining plus specific whole chromosome painting allows simultaneous and objective detection of complete or incomplete chromosome exchanges and interstitial or terminal deletions in human peripheral lymphocytes. Of the six proposed cytogenetic ratios, the I-ratio is the most effective cytogenetic fingerprint for distinguishing low-LET from high-LET radiation in human metaphase human lymphocytes.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09553002.aspen_US
dc.relation.ispartofInternational Journal of Radiation Biologyen_US
dc.subject.meshBeta Particlesen_US
dc.subject.meshChromosome Aberrationsen_US
dc.subject.meshChromosome Paintingen_US
dc.subject.meshChromosomes - Radiation Effects - Ultrastructureen_US
dc.subject.meshCytogeneticsen_US
dc.subject.meshDna Fingerprintingen_US
dc.subject.meshGamma Raysen_US
dc.subject.meshGenetic Techniquesen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshLinear Energy Transferen_US
dc.subject.meshLymphocytes - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMetaphaseen_US
dc.subject.meshNeutronsen_US
dc.subject.meshTelomere - Ultrastructureen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTranslocation, Geneticen_US
dc.titleA comparative study on potential cytogenetic fingerprints for radiation LET in human lymphocytesen_US
dc.typeArticleen_US
dc.identifier.emailDeng, W: wdeng@hkucc.hku.hken_US
dc.identifier.authorityDeng, W=rp01640en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11133040-
dc.identifier.scopuseid_2-s2.0-0033639194en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033639194&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume76en_US
dc.identifier.issue12en_US
dc.identifier.spage1589en_US
dc.identifier.epage1598en_US
dc.identifier.isiWOS:000165906800002-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridDeng, W=7202223673en_US
dc.identifier.scopusauthoridMorrison, DP=7402118438en_US
dc.identifier.scopusauthoridGale, KL=35510051700en_US
dc.identifier.scopusauthoridLucas, JN=7402441937en_US

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