File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Biological dosimetry of beta-ray exposure from tritium using chromosome translocations in human lymphocytes analyzed by fluorescence in situ hybridization

TitleBiological dosimetry of beta-ray exposure from tritium using chromosome translocations in human lymphocytes analyzed by fluorescence in situ hybridization
Authors
Issue Date1998
Citation
Radiation Research, 1998, v. 150 n. 4, p. 400-405 How to Cite?
AbstractRadiation exposures from tritium make up a substantial fraction of the occupational and accidental radiation exposures associated with the nuclear power industry. Tritiated water, the most abundant form of tritium, is of particular interest because it is readily taken up by human cells and its irradiation of the cells is spread over a period of days. To approximate the prolonged exposure and the conditions that the cells of an individual would experience in vivo, we irradiated human lymphocytes with tritiated water for 48 h in a 1:1 blood:medium mix. For estimation of the tritium β-ray dose, a cellular water content of 0.78, based on measurements of human lymphoblastoid cells in culture medium, was used. A modified dose calculation formula was developed for the radiation exposure conditions. A total of 48,014 metaphases (14,482 in irradiated samples and 33,532 in control, unirradiated samples) in human lymphocytes cultured for 72 h after exposure were analyzed for chromosome translocations using fluorescence in situ hybridization. The linear slope (α coefficient) of the dose-response curve was measured to be (3.93 ± 0.42) X 10 -2 and (5.26 ± 0.48) X 10 -2 translocations per cell per gray for complete translocations (tc) and complete translocations plus incomplete translocations [ti(Ab)], respectively, when the data were fitted to a linear model using a weighted least-squares method. The α coefficient for tc is significantly lower than that for conventionally measured dicentrics after tritium β irradiation, but the α coefficient for tc + ti(Ab) does not differ significantly from that for dicentrics. This is in agreement with theoretical considerations. The importance of scoring criteria is stressed. The frequency of tc + ti(Ab) is proposed to be a reliable biodosimeter for tritium exposures, and its practical use in a dose reconstruction is presented.
Persistent Identifierhttp://hdl.handle.net/10722/178255
ISSN
2015 Impact Factor: 3.022
2015 SCImago Journal Rankings: 1.493
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDeng, Wen_US
dc.contributor.authorMorrison, DPen_US
dc.contributor.authorGale, KLen_US
dc.contributor.authorLucas, JNen_US
dc.date.accessioned2012-12-19T09:44:47Z-
dc.date.available2012-12-19T09:44:47Z-
dc.date.issued1998en_US
dc.identifier.citationRadiation Research, 1998, v. 150 n. 4, p. 400-405en_US
dc.identifier.issn0033-7587en_US
dc.identifier.urihttp://hdl.handle.net/10722/178255-
dc.description.abstractRadiation exposures from tritium make up a substantial fraction of the occupational and accidental radiation exposures associated with the nuclear power industry. Tritiated water, the most abundant form of tritium, is of particular interest because it is readily taken up by human cells and its irradiation of the cells is spread over a period of days. To approximate the prolonged exposure and the conditions that the cells of an individual would experience in vivo, we irradiated human lymphocytes with tritiated water for 48 h in a 1:1 blood:medium mix. For estimation of the tritium β-ray dose, a cellular water content of 0.78, based on measurements of human lymphoblastoid cells in culture medium, was used. A modified dose calculation formula was developed for the radiation exposure conditions. A total of 48,014 metaphases (14,482 in irradiated samples and 33,532 in control, unirradiated samples) in human lymphocytes cultured for 72 h after exposure were analyzed for chromosome translocations using fluorescence in situ hybridization. The linear slope (α coefficient) of the dose-response curve was measured to be (3.93 ± 0.42) X 10 -2 and (5.26 ± 0.48) X 10 -2 translocations per cell per gray for complete translocations (tc) and complete translocations plus incomplete translocations [ti(Ab)], respectively, when the data were fitted to a linear model using a weighted least-squares method. The α coefficient for tc is significantly lower than that for conventionally measured dicentrics after tritium β irradiation, but the α coefficient for tc + ti(Ab) does not differ significantly from that for dicentrics. This is in agreement with theoretical considerations. The importance of scoring criteria is stressed. The frequency of tc + ti(Ab) is proposed to be a reliable biodosimeter for tritium exposures, and its practical use in a dose reconstruction is presented.en_US
dc.languageengen_US
dc.relation.ispartofRadiation Researchen_US
dc.subject.meshBeta Particlesen_US
dc.subject.meshDose-Response Relationship, Radiationen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshLymphocytes - Radiation Effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshRadiation Dosageen_US
dc.subject.meshTranslocation, Geneticen_US
dc.subject.meshTritiumen_US
dc.titleBiological dosimetry of beta-ray exposure from tritium using chromosome translocations in human lymphocytes analyzed by fluorescence in situ hybridizationen_US
dc.typeArticleen_US
dc.identifier.emailDeng, W: wdeng@hkucc.hku.hken_US
dc.identifier.authorityDeng, W=rp01640en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2307/3579658en_US
dc.identifier.pmid9768853-
dc.identifier.scopuseid_2-s2.0-0031680671en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031680671&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume150en_US
dc.identifier.issue4en_US
dc.identifier.spage400en_US
dc.identifier.epage405en_US
dc.identifier.isiWOS:000076202700004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDeng, W=7202223673en_US
dc.identifier.scopusauthoridMorrison, DP=7402118438en_US
dc.identifier.scopusauthoridGale, KL=35510051700en_US
dc.identifier.scopusauthoridLucas, JN=7402441937en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats