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Article: The effect of naringin on early growth and development of the spheno-occipital synchondrosis as measured by the expression of PTHrP and Sox9-an in vitro model

TitleThe effect of naringin on early growth and development of the spheno-occipital synchondrosis as measured by the expression of PTHrP and Sox9-an in vitro model
Authors
Issue Date2013
PublisherOxford University Press. The Journal's web site is located at http://ejo.oxfordjournals.org/
Citation
European Journal of Orthodontics, 2013, v. 35 n. 6, p. 826-831 How to Cite?
AbstractThe aim of this study was to assess the effect of the flavonoid naringin on the growth of the spheno-occipital synchondrosis by quantifying the levels of expression of Sox9 and PTHrP in an in vitro mouse model. Fifty 1-day-old BALB/c mice were randomly assigned to experimental or control groups, and each group equally divided into five time frames (6, 24, 48, 72 and 168 hours). The mice were sacrificed with phenobarbitone sodium, and the spheno-occipital synchondroses dissected and cultured in control or experimental medium, with the experimental medium supplemented with 0.1 microm naringin. Sections of the specimens underwent immunohistochemical staining for Sox9 and PTHrP, and the amount of expression was quantified using true-colour RGB (red-green-blue) computer-assisted image-analysing system with digital imaging. Data analysis showed there was a significant increase of expression of Sox9 at 6 and 24 hours (P < 0.001) between experimental and control groups, however, there was no significant difference between the levels of expression of PTHrP between experimental and control groups at any of the time frames. There was a very weak correlation found in this study between the expression of PTHrP and Sox9. In conclusion, naringin enhances the growth of the spheno-occipital synchondrosis through over expression of Sox9. This is a successful in vitro model to study factors regulating the growth of the spheno-occipital synchondrosis.
Persistent Identifierhttp://hdl.handle.net/10722/177331
ISSN
2015 Impact Factor: 1.44
2015 SCImago Journal Rankings: 1.090
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNowak-Solinska, Een_US
dc.contributor.authorRabie, ABMen_US
dc.contributor.authorWong, RWKen_US
dc.contributor.authorLei, SWYen_US
dc.date.accessioned2012-12-18T05:01:09Z-
dc.date.available2012-12-18T05:01:09Z-
dc.date.issued2013en_US
dc.identifier.citationEuropean Journal of Orthodontics, 2013, v. 35 n. 6, p. 826-831en_US
dc.identifier.issn0141-5387-
dc.identifier.urihttp://hdl.handle.net/10722/177331-
dc.description.abstractThe aim of this study was to assess the effect of the flavonoid naringin on the growth of the spheno-occipital synchondrosis by quantifying the levels of expression of Sox9 and PTHrP in an in vitro mouse model. Fifty 1-day-old BALB/c mice were randomly assigned to experimental or control groups, and each group equally divided into five time frames (6, 24, 48, 72 and 168 hours). The mice were sacrificed with phenobarbitone sodium, and the spheno-occipital synchondroses dissected and cultured in control or experimental medium, with the experimental medium supplemented with 0.1 microm naringin. Sections of the specimens underwent immunohistochemical staining for Sox9 and PTHrP, and the amount of expression was quantified using true-colour RGB (red-green-blue) computer-assisted image-analysing system with digital imaging. Data analysis showed there was a significant increase of expression of Sox9 at 6 and 24 hours (P < 0.001) between experimental and control groups, however, there was no significant difference between the levels of expression of PTHrP between experimental and control groups at any of the time frames. There was a very weak correlation found in this study between the expression of PTHrP and Sox9. In conclusion, naringin enhances the growth of the spheno-occipital synchondrosis through over expression of Sox9. This is a successful in vitro model to study factors regulating the growth of the spheno-occipital synchondrosis.-
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://ejo.oxfordjournals.org/-
dc.relation.ispartofEuropean Journal of Orthodonticsen_US
dc.titleThe effect of naringin on early growth and development of the spheno-occipital synchondrosis as measured by the expression of PTHrP and Sox9-an in vitro modelen_US
dc.typeArticleen_US
dc.identifier.emailRabie, ABM: rabie@hku.hken_US
dc.identifier.emailWong, RWK: fyoung@hku.hken_US
dc.identifier.authorityRabie, ABM=rp00029en_US
dc.identifier.authorityWong, RWK=rp00038en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/ejo/cjs089en_US
dc.identifier.pmid23172577-
dc.identifier.hkuros212646en_US
dc.identifier.isiWOS:000327718200015-
dc.publisher.placeUnited Kingdom-

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