File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A new method of culturing and transferring IRIS pigment epithelium

TitleA new method of culturing and transferring IRIS pigment epithelium
Authors
Issue Date1997
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 1997, v. 38 n. 4, p. S335 How to Cite?
AbstractPurpose. To optimize the method of c ilturing and transferring of iris pigment epithelial (IPE) cells for cellular studie; in vitro or for transplantation in vivo. Methods. Porcine iris tissues were obtiined and IPE cells were isolated and cultured at high densities by plating them in a drop-form fashion. After seven to ten days, spherical-shaped structures conta rung high concentration of cells were obtained. Subcultures of these cells wei e performed by direct pipetting of the spheres into new culture dishes without employing enzymatic dissociation of the spheres from the plates. Immunohistochemical and light and electron microscopic analyses were carried out. Results: IPE cells, when cultured at high densities, tended to form elevated spherical structures containing viable colls. The cultured cells were pigmented and showed positive labeling with monoclonal cytokeratin antibody. They proliferated and migrated from the spheres to form nonolayers. The spheres could be easily aspirated and transferred from one culture dish to another. IPE cells originating from the transferred spheres continued to proliferate and migrate in a similar fashion to the originally cultivated cells to form monolayers after seven to ten days. Conclusions: This new method provices a simple means of cultunng a high quantity of IPE cells. Its high yield of lure IPE cells and the ease of cell transfer provide an ideal source for their deliveiy into the subretinal space in vivo or tor their study at the cellular level in vitro.
Persistent Identifierhttp://hdl.handle.net/10722/176455
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008

 

DC FieldValueLanguage
dc.contributor.authorLai, Wen_US
dc.contributor.authorRezaei, KAen_US
dc.contributor.authorFarrokhSiar, Len_US
dc.date.accessioned2012-11-26T09:11:23Z-
dc.date.available2012-11-26T09:11:23Z-
dc.date.issued1997en_US
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 1997, v. 38 n. 4, p. S335en_US
dc.identifier.issn0146-0404en_US
dc.identifier.urihttp://hdl.handle.net/10722/176455-
dc.description.abstractPurpose. To optimize the method of c ilturing and transferring of iris pigment epithelial (IPE) cells for cellular studie; in vitro or for transplantation in vivo. Methods. Porcine iris tissues were obtiined and IPE cells were isolated and cultured at high densities by plating them in a drop-form fashion. After seven to ten days, spherical-shaped structures conta rung high concentration of cells were obtained. Subcultures of these cells wei e performed by direct pipetting of the spheres into new culture dishes without employing enzymatic dissociation of the spheres from the plates. Immunohistochemical and light and electron microscopic analyses were carried out. Results: IPE cells, when cultured at high densities, tended to form elevated spherical structures containing viable colls. The cultured cells were pigmented and showed positive labeling with monoclonal cytokeratin antibody. They proliferated and migrated from the spheres to form nonolayers. The spheres could be easily aspirated and transferred from one culture dish to another. IPE cells originating from the transferred spheres continued to proliferate and migrate in a similar fashion to the originally cultivated cells to form monolayers after seven to ten days. Conclusions: This new method provices a simple means of cultunng a high quantity of IPE cells. Its high yield of lure IPE cells and the ease of cell transfer provide an ideal source for their deliveiy into the subretinal space in vivo or tor their study at the cellular level in vitro.en_US
dc.languageengen_US
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_US
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_US
dc.titleA new method of culturing and transferring IRIS pigment epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailLai, W: wicolai@hku.hken_US
dc.identifier.authorityLai, W=rp00531en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33749118886en_US
dc.identifier.volume38en_US
dc.identifier.issue4en_US
dc.identifier.spageS335en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLai, W=7402231098en_US
dc.identifier.scopusauthoridRezaei, KA=12792744000en_US
dc.identifier.scopusauthoridFarrokhSiar, L=6602082268en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats