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Article: Effect of melanin on traumatic hyphema in rabbits

TitleEffect of melanin on traumatic hyphema in rabbits
Authors
Issue Date1999
PublisherAmerican Medical Association. The Journal's web site is located at http://www.archopthalmol.com
Citation
Archives Of Ophthalmology, 1999, v. 117 n. 6, p. 789-793 How to Cite?
AbstractObjective: To investigate the role of melanin in influencing the clearance of traumatic hyphema and in the incidence of rebleeds following the hyphemas. Methods: Hyphemas were induced in 30 eyes of New Zealand white albino rabbits using an Nd:YAG laser. A total of 3.75 mg of synthetic melanin suspended in 0.1 mL of balanced salt solution was introduced into the anterior chambers of 16 animals. A total of 0.1 mL of balanced salt solution was injected into 14 control eyes. Hyphema levels were measured by a masked observer (V.D.B.) daily for 15 days. Pairs of animals were sacrificed at 1, 3, 5, 10, and 15 days and the eyes studied histologically. Results: Hyphemas were consistently produced in all eyes with mean ± SD levels of 1.44 ± 0.22 mm and 1.57 ± 0.24 mm in the melanin-treated and control eyes, respectively. The clearance of hyphemas in the melanin-treated eyes was significantly prolonged throughout the study (P < .001). The rate of rebleed in the melanin-treated group was 18.8% and in the control group was 7.1% (P < .01). Histologically, both groups showed variable degrees of blood in the anterior chambers and trabecular meshwork. In addition, the melanin-treated eyes showed free melanin, melanin-laden macrophages, and an inflammatory response in the anterior chamber and trabecular meshwork that was greater than that in the control eyes. Melanin-treated eyes with rebleeds showed organized hemorrhage with neovascularization. Conclusions: The presence of melanin results in a significantly prolonged course of hyphemas and may influence the rate of rebleeds. Occlusion of the trabecular meshwork with melanin-laden macrophages and inflammation may be the mechanisms responsible for these effects. Clinical Relevance: The release of melanin into the anterior chamber during ocular trauma may be partly responsible for the susceptibility of darker-pigmented individuals to more serious complications following a traumatic hyphema.
Persistent Identifierhttp://hdl.handle.net/10722/176356
ISSN
2014 Impact Factor: 4.399
References

 

DC FieldValueLanguage
dc.contributor.authorLai, WWen_US
dc.contributor.authorBhavnani, VDen_US
dc.contributor.authorTessler, HHen_US
dc.contributor.authorEdward, DPen_US
dc.date.accessioned2012-11-26T09:10:47Z-
dc.date.available2012-11-26T09:10:47Z-
dc.date.issued1999en_US
dc.identifier.citationArchives Of Ophthalmology, 1999, v. 117 n. 6, p. 789-793en_US
dc.identifier.issn0003-9950en_US
dc.identifier.urihttp://hdl.handle.net/10722/176356-
dc.description.abstractObjective: To investigate the role of melanin in influencing the clearance of traumatic hyphema and in the incidence of rebleeds following the hyphemas. Methods: Hyphemas were induced in 30 eyes of New Zealand white albino rabbits using an Nd:YAG laser. A total of 3.75 mg of synthetic melanin suspended in 0.1 mL of balanced salt solution was introduced into the anterior chambers of 16 animals. A total of 0.1 mL of balanced salt solution was injected into 14 control eyes. Hyphema levels were measured by a masked observer (V.D.B.) daily for 15 days. Pairs of animals were sacrificed at 1, 3, 5, 10, and 15 days and the eyes studied histologically. Results: Hyphemas were consistently produced in all eyes with mean ± SD levels of 1.44 ± 0.22 mm and 1.57 ± 0.24 mm in the melanin-treated and control eyes, respectively. The clearance of hyphemas in the melanin-treated eyes was significantly prolonged throughout the study (P < .001). The rate of rebleed in the melanin-treated group was 18.8% and in the control group was 7.1% (P < .01). Histologically, both groups showed variable degrees of blood in the anterior chambers and trabecular meshwork. In addition, the melanin-treated eyes showed free melanin, melanin-laden macrophages, and an inflammatory response in the anterior chamber and trabecular meshwork that was greater than that in the control eyes. Melanin-treated eyes with rebleeds showed organized hemorrhage with neovascularization. Conclusions: The presence of melanin results in a significantly prolonged course of hyphemas and may influence the rate of rebleeds. Occlusion of the trabecular meshwork with melanin-laden macrophages and inflammation may be the mechanisms responsible for these effects. Clinical Relevance: The release of melanin into the anterior chamber during ocular trauma may be partly responsible for the susceptibility of darker-pigmented individuals to more serious complications following a traumatic hyphema.en_US
dc.languageengen_US
dc.publisherAmerican Medical Association. The Journal's web site is located at http://www.archopthalmol.comen_US
dc.relation.ispartofArchives of Ophthalmologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnterior Chamber - Drug Effects - Pathologyen_US
dc.subject.meshEye Injuries - Complications - Pathology - Physiopathologyen_US
dc.subject.meshHyphema - Etiology - Pathology - Physiopathologyen_US
dc.subject.meshIris - Blood Supply - Injuries - Pathologyen_US
dc.subject.meshMacrophages - Pathologyen_US
dc.subject.meshMelanins - Pharmacologyen_US
dc.subject.meshRabbitsen_US
dc.subject.meshRecurrenceen_US
dc.titleEffect of melanin on traumatic hyphema in rabbitsen_US
dc.typeArticleen_US
dc.identifier.emailLai, WW: wicolai@hku.hken_US
dc.identifier.authorityLai, WW=rp00531en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1001/archopht.117.6.789-
dc.identifier.pmid10369591-
dc.identifier.scopuseid_2-s2.0-0032989296en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032989296&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume117en_US
dc.identifier.issue6en_US
dc.identifier.spage789en_US
dc.identifier.epage793en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLai, WW=7402231098en_US
dc.identifier.scopusauthoridBhavnani, VD=14719230500en_US
dc.identifier.scopusauthoridTessler, HH=7003279432en_US
dc.identifier.scopusauthoridEdward, DP=7005234763en_US

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