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Article: The human lysozyme promoter directs reporter gene expression to activated myelomonocytic cells in transgenic mice

TitleThe human lysozyme promoter directs reporter gene expression to activated myelomonocytic cells in transgenic mice
Authors
Issue Date1996
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 1996, v. 93 n. 4, p. 1434-1438 How to Cite?
AbstractThe 5' region of the human lysozyme gene from -3500 to +25 was fused to a chloramphenicol acetyltransferase (CAT) reporter gene and three transgenic founder mice were obtained. All three transgenic lines showed the same pattern of CAT enzyme expression in adult mouse tissues that was consistent with the targeting of elicited, activated macrophages in tissues and developing and elicited granulocytes. In normal mice high CAT enzyme activity was found in the spleen, lung, and thymus, tissues rich in phagocytically active cells, but nut in many other tissues, such as the gut and muscle, which contain resident macrophages. Cultured resident peritoneal macrophages and cells elicited 18 hr (granulocytes) and 4 days (macrophages) after injection of sterile thioglycollate broth expressed CAT activity. Bacillus Calmette-Guerin infection of transgenic mice resulted in CAT enzyme expression in the liver, which contained macrophage-rich granulomas, whereas the liver of uninfected mice did not have any detectable CAT enzyme activity. Although the Paneth cells of the small intestine in both human and mouse produce lysozyme, the CAT gene, under the control of the human lysozyme promoter, was not expressed in the mouse small intestine. These results indicate that the human lysozyme promoter region may be used to direct expression of genes to activated mouse myeloid cells.
Persistent Identifierhttp://hdl.handle.net/10722/176002
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorClarke, Sen_US
dc.contributor.authorGreaves, DRen_US
dc.contributor.authorChung, LPen_US
dc.contributor.authorTree, Pen_US
dc.contributor.authorGordon, Sen_US
dc.date.accessioned2012-11-26T09:04:04Z-
dc.date.available2012-11-26T09:04:04Z-
dc.date.issued1996en_US
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 1996, v. 93 n. 4, p. 1434-1438en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/176002-
dc.description.abstractThe 5' region of the human lysozyme gene from -3500 to +25 was fused to a chloramphenicol acetyltransferase (CAT) reporter gene and three transgenic founder mice were obtained. All three transgenic lines showed the same pattern of CAT enzyme expression in adult mouse tissues that was consistent with the targeting of elicited, activated macrophages in tissues and developing and elicited granulocytes. In normal mice high CAT enzyme activity was found in the spleen, lung, and thymus, tissues rich in phagocytically active cells, but nut in many other tissues, such as the gut and muscle, which contain resident macrophages. Cultured resident peritoneal macrophages and cells elicited 18 hr (granulocytes) and 4 days (macrophages) after injection of sterile thioglycollate broth expressed CAT activity. Bacillus Calmette-Guerin infection of transgenic mice resulted in CAT enzyme expression in the liver, which contained macrophage-rich granulomas, whereas the liver of uninfected mice did not have any detectable CAT enzyme activity. Although the Paneth cells of the small intestine in both human and mouse produce lysozyme, the CAT gene, under the control of the human lysozyme promoter, was not expressed in the mouse small intestine. These results indicate that the human lysozyme promoter region may be used to direct expression of genes to activated mouse myeloid cells.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChloramphenicol O-Acetyltransferase - Biosynthesis - Geneticsen_US
dc.subject.meshGene Expression Regulation - Geneticsen_US
dc.subject.meshGenes, Reporteren_US
dc.subject.meshGranulocytes - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshIntestine, Small - Enzymologyen_US
dc.subject.meshMacrophage Activation - Geneticsen_US
dc.subject.meshMacrophages, Peritoneal - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshMuramidase - Geneticsen_US
dc.subject.meshMycobacterium Bovisen_US
dc.subject.meshOrgan Specificityen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRecombinant Fusion Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTuberculoma - Enzymology - Pathologyen_US
dc.subject.meshTuberculosis, Hepatic - Enzymology - Pathologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleThe human lysozyme promoter directs reporter gene expression to activated myelomonocytic cells in transgenic miceen_US
dc.typeArticleen_US
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_US
dc.identifier.authorityChung, LP=rp00249en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1073/pnas.93.4.1434en_US
dc.identifier.pmid8643649-
dc.identifier.scopuseid_2-s2.0-0029981424en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029981424&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume93en_US
dc.identifier.issue4en_US
dc.identifier.spage1434en_US
dc.identifier.epage1438en_US
dc.identifier.isiWOS:A1996TW69800015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridClarke, S=8072569200en_US
dc.identifier.scopusauthoridGreaves, DR=24762846800en_US
dc.identifier.scopusauthoridChung, LP=24315879100en_US
dc.identifier.scopusauthoridTree, P=6506340209en_US
dc.identifier.scopusauthoridGordon, S=35391350600en_US

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